Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P57132 (MNME_BUCAI)

Last modified November 3, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    tRNA modification GTPase mnmE
    EC=3.6.-.-
Gene names
Name: mnmE
Synonyms: thdF, trmE
Ordered Locus Names: BU016
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]
Taxonomic identifier118099 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Hide | Top

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.

Cofactor

Binds 1 potassium ion per subunit By similarity.

Subunit structure

Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Hide | Top

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

potassium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452tRNA modification GTPase mnmE HAMAP MF_00379
PRO_0000188856

Regions

Nucleotide binding225 – 2306GTP By similarity
Nucleotide binding244 – 2507GTP By similarity
Nucleotide binding269 – 2724GTP By similarity
Nucleotide binding357 – 3593GTP By similarity

Sites

Metal binding2251Potassium By similarity
Metal binding2291Magnesium By similarity
Metal binding2441Potassium; via carbonyl oxygen By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2491Potassium By similarity
Metal binding2501Magnesium By similarity
Binding site221Formyltetrahydrofolate By similarity
Binding site791Formyltetrahydrofolate By similarity
Binding site1191Formyltetrahydrofolate By similarity
Binding site4521Formyltetrahydrofolate By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P57132-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: CAF465790A479165

FASTA45251,080
        10         20         30         40         50         60 
MRNETIVAQV TCPGKSAVGI LRISGFQTKI VAIKILGKIP LARFATYSNF LDENNEILDK 

        70         80         90        100        110        120 
GISLWFPAPH SLTGEDVLEL QGHGSPLIMD LLIRRILSIN NIRMAKPGEF SERAFLNGKM 

       130        140        150        160        170        180 
DLIQAESIDD LINSETELSA RASLNSLQGK FSFFIKELMN LIIEFRINIE SNIDFSEEEI 

       190        200        210        220        230        240 
NINMQDIINI KFRELHDKFL KLKATVLKGS LLREGKKIVI AGLPNSGKSS LLNILSYSDR 

       250        260        270        280        290        300 
AIVTDIPGTT RDLLYEDISI NGVRCELIDT AGLRDTDDKV ERIGIMRAWE MIRKSDHVLF 

       310        320        330        340        350        360 
VIDKTLSKSK QKKICDDFMK NILNNKTQVT FVLNKNDLIK DEFGIKEIDG TAFISISART 

       370        380        390        400        410        420 
GEGVNILREH IIKIEKNINN ESVFIARRRH IKQLDLAYDE FLTAENTWRE SENIELLAES 

       430        440        450 
LSIISKFLGE IIGQCTSEDL LNHIFSSFCI GK

« Hide

Hide | Top

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tokyo 1998.

Hide | Top

Cross-references

Sequence databases

BA000003 Genomic DNA. Translation: BAB12744.1.
RefSeqNP_239858.2.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1109525.
GenomeReviewsGene locus BU016 in contig BA000003_GR.
KEGGbuc:BU016.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP57132.
OMAKGPNSFT

Enzyme and pathway databases

BioCycBSP107806:BU016-MON.

Family and domain databases

HAMAPMF_00379.
[Tree]
InterProIPR018948. GTP_bd_TrmE_N.
IPR002917. MMR_HSR1_GTP_bd.
IPR005225. Small_GTP_bd.
IPR004520. ThdF.
[Graphical view]
Gene3DG3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
PfamPF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameMNME_BUCAI
AccessionPrimary (citable) accession number: P57132
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents