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P57132 (MNME_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE

EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:thdF, trmE
Ordered Locus Names:BU016
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity. HAMAP-Rule MF_00379

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00379

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00379.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452tRNA modification GTPase MnmE HAMAP-Rule MF_00379
PRO_0000188856

Regions

Domain217 – 335119G
Nucleotide binding225 – 2306GTP By similarity
Nucleotide binding244 – 2507GTP By similarity
Nucleotide binding269 – 2724GTP By similarity
Nucleotide binding357 – 3593GTP By similarity

Sites

Metal binding2251Potassium By similarity
Metal binding2291Magnesium By similarity
Metal binding2441Potassium; via carbonyl oxygen By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2491Potassium By similarity
Metal binding2501Magnesium By similarity
Binding site221Formyltetrahydrofolate By similarity
Binding site791Formyltetrahydrofolate By similarity
Binding site1191Formyltetrahydrofolate By similarity
Binding site4521Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
P57132 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: CAF465790A479165

FASTA45251,080
        10         20         30         40         50         60 
MRNETIVAQV TCPGKSAVGI LRISGFQTKI VAIKILGKIP LARFATYSNF LDENNEILDK 

        70         80         90        100        110        120 
GISLWFPAPH SLTGEDVLEL QGHGSPLIMD LLIRRILSIN NIRMAKPGEF SERAFLNGKM 

       130        140        150        160        170        180 
DLIQAESIDD LINSETELSA RASLNSLQGK FSFFIKELMN LIIEFRINIE SNIDFSEEEI 

       190        200        210        220        230        240 
NINMQDIINI KFRELHDKFL KLKATVLKGS LLREGKKIVI AGLPNSGKSS LLNILSYSDR 

       250        260        270        280        290        300 
AIVTDIPGTT RDLLYEDISI NGVRCELIDT AGLRDTDDKV ERIGIMRAWE MIRKSDHVLF 

       310        320        330        340        350        360 
VIDKTLSKSK QKKICDDFMK NILNNKTQVT FVLNKNDLIK DEFGIKEIDG TAFISISART 

       370        380        390        400        410        420 
GEGVNILREH IIKIEKNINN ESVFIARRRH IKQLDLAYDE FLTAENTWRE SENIELLAES 

       430        440        450 
LSIISKFLGE IIGQCTSEDL LNHIFSSFCI GK 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12744.1.
RefSeqNP_239858.2. NC_002528.1.

3D structure databases

ProteinModelPortalP57132.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12744; BAB12744; BAB12744.
GeneID1109525.
KEGGbuc:BU016.
PATRIC21243536. VBIBucAph127364_0029.

Phylogenomic databases

eggNOGCOG0486.
HOGENOMHOG000200714.
KOK03650.
OMASIMDEGI.
OrthoDBEOG6DC6K1.
ProtClustDBPRK05291.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-16-MONOMER.

Family and domain databases

Gene3D1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00379. GTPase_MnmE.
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
TIGRFAMsTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_BUCAI
AccessionPrimary (citable) accession number: P57132
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names