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P57132

- MNME_BUCAI

UniProt

P57132 - MNME_BUCAI

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Protein

tRNA modification GTPase MnmE

Gene
mnmE, thdF, trmE, BU016
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.UniRule annotation

Cofactori

Binds 1 potassium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Formyltetrahydrofolate By similarity
Binding sitei79 – 791Formyltetrahydrofolate By similarity
Binding sitei119 – 1191Formyltetrahydrofolate By similarity
Metal bindingi225 – 2251Potassium By similarity
Metal bindingi229 – 2291Magnesium By similarity
Metal bindingi244 – 2441Potassium; via carbonyl oxygen By similarity
Metal bindingi246 – 2461Potassium; via carbonyl oxygen By similarity
Metal bindingi249 – 2491Potassium By similarity
Metal bindingi250 – 2501Magnesium By similarity
Binding sitei452 – 4521Formyltetrahydrofolate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi225 – 2306GTP By similarity
Nucleotide bindingi244 – 2507GTP By similarity
Nucleotide bindingi269 – 2724GTP By similarity
Nucleotide bindingi357 – 3593GTP By similarity

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-16-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmE (EC:3.6.-.-)
Gene namesi
Name:mnmE
Synonyms:thdF, trmE
Ordered Locus Names:BU016
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452tRNA modification GTPase MnmEUniRule annotationPRO_0000188856Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57132.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini215 – 376162TrmE-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0486.
HOGENOMiHOG000200714.
KOiK03650.
OMAiGVQSELY.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57132-1 [UniParc]FASTAAdd to Basket

« Hide

MRNETIVAQV TCPGKSAVGI LRISGFQTKI VAIKILGKIP LARFATYSNF    50
LDENNEILDK GISLWFPAPH SLTGEDVLEL QGHGSPLIMD LLIRRILSIN 100
NIRMAKPGEF SERAFLNGKM DLIQAESIDD LINSETELSA RASLNSLQGK 150
FSFFIKELMN LIIEFRINIE SNIDFSEEEI NINMQDIINI KFRELHDKFL 200
KLKATVLKGS LLREGKKIVI AGLPNSGKSS LLNILSYSDR AIVTDIPGTT 250
RDLLYEDISI NGVRCELIDT AGLRDTDDKV ERIGIMRAWE MIRKSDHVLF 300
VIDKTLSKSK QKKICDDFMK NILNNKTQVT FVLNKNDLIK DEFGIKEIDG 350
TAFISISART GEGVNILREH IIKIEKNINN ESVFIARRRH IKQLDLAYDE 400
FLTAENTWRE SENIELLAES LSIISKFLGE IIGQCTSEDL LNHIFSSFCI 450
GK 452
Length:452
Mass (Da):51,080
Last modified:December 1, 2000 - v1
Checksum:iCAF465790A479165
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12744.1.
RefSeqiNP_239858.2. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12744; BAB12744; BAB12744.
GeneIDi1109525.
KEGGibuc:BU016.
PATRICi21243536. VBIBucAph127364_0029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12744.1 .
RefSeqi NP_239858.2. NC_002528.1.

3D structure databases

ProteinModelPortali P57132.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12744 ; BAB12744 ; BAB12744 .
GeneIDi 1109525.
KEGGi buc:BU016.
PATRICi 21243536. VBIBucAph127364_0029.

Phylogenomic databases

eggNOGi COG0486.
HOGENOMi HOG000200714.
KOi K03650.
OMAi GVQSELY.
OrthoDBi EOG6DC6K1.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-16-MONOMER.

Family and domain databases

Gene3Di 1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00379. GTPase_MnmE.
InterProi IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view ]
Pfami PF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS51709. G_TRME. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiMNME_BUCAI
AccessioniPrimary (citable) accession number: P57132
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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