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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22FormyltetrahydrofolateUniRule annotation1
Binding sitei79FormyltetrahydrofolateUniRule annotation1
Binding sitei119FormyltetrahydrofolateUniRule annotation1
Metal bindingi225PotassiumUniRule annotation1
Metal bindingi229MagnesiumUniRule annotation1
Metal bindingi244Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi246Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi249PotassiumUniRule annotation1
Metal bindingi250MagnesiumUniRule annotation1
Binding sitei452FormyltetrahydrofolateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi225 – 230GTPUniRule annotation6
Nucleotide bindingi244 – 250GTPUniRule annotation7
Nucleotide bindingi269 – 272GTPUniRule annotation4
Nucleotide bindingi357 – 359GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:thdFUniRule annotation, trmEUniRule annotation
Ordered Locus Names:BU016
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001888561 – 452tRNA modification GTPase MnmEAdd BLAST452

Proteomic databases

PRIDEiP57132

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi107806.BU016

Structurei

3D structure databases

ProteinModelPortaliP57132
SMRiP57132
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 376TrmE-type GAdd BLAST162

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H Bacteria
COG0486 LUCA
HOGENOMiHOG000200714
KOiK03650
OMAiEFTQRAF

Family and domain databases

CDDicd04164 trmE, 1 hit
Gene3Di1.20.120.430, 2 hits
3.30.1360.120, 1 hit
HAMAPiMF_00379 GTPase_MnmE, 1 hit
InterProiView protein in InterPro
IPR031168 G_TrmE
IPR018948 GTP-bd_TrmE_N
IPR006073 GTP_binding_domain
IPR004520 GTPase_MnmE
IPR027368 MnmE_dom2
IPR025867 MnmE_helical
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR027266 TrmE/GcvT_dom1
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
PF12631 MnmE_helical, 1 hit
PF10396 TrmE_N, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00450 mnmE_trmE_thdF, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51709 G_TRME, 1 hit

Sequencei

Sequence statusi: Complete.

P57132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNETIVAQV TCPGKSAVGI LRISGFQTKI VAIKILGKIP LARFATYSNF
60 70 80 90 100
LDENNEILDK GISLWFPAPH SLTGEDVLEL QGHGSPLIMD LLIRRILSIN
110 120 130 140 150
NIRMAKPGEF SERAFLNGKM DLIQAESIDD LINSETELSA RASLNSLQGK
160 170 180 190 200
FSFFIKELMN LIIEFRINIE SNIDFSEEEI NINMQDIINI KFRELHDKFL
210 220 230 240 250
KLKATVLKGS LLREGKKIVI AGLPNSGKSS LLNILSYSDR AIVTDIPGTT
260 270 280 290 300
RDLLYEDISI NGVRCELIDT AGLRDTDDKV ERIGIMRAWE MIRKSDHVLF
310 320 330 340 350
VIDKTLSKSK QKKICDDFMK NILNNKTQVT FVLNKNDLIK DEFGIKEIDG
360 370 380 390 400
TAFISISART GEGVNILREH IIKIEKNINN ESVFIARRRH IKQLDLAYDE
410 420 430 440 450
FLTAENTWRE SENIELLAES LSIISKFLGE IIGQCTSEDL LNHIFSSFCI

GK
Length:452
Mass (Da):51,080
Last modified:December 1, 2000 - v1
Checksum:iCAF465790A479165
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA Translation: BAB12744.1
RefSeqiNP_239858.2, NC_002528.1

Genome annotation databases

EnsemblBacteriaiBAB12744; BAB12744; BAB12744
GeneIDi1109525
KEGGibuc:BU016
PATRICifig|107806.10.peg.29

Similar proteinsi

Entry informationi

Entry nameiMNME_BUCAI
AccessioniPrimary (citable) accession number: P57132
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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