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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

K(+)UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221FormyltetrahydrofolateUniRule annotation
Binding sitei79 – 791FormyltetrahydrofolateUniRule annotation
Binding sitei119 – 1191FormyltetrahydrofolateUniRule annotation
Metal bindingi225 – 2251PotassiumUniRule annotation
Metal bindingi229 – 2291MagnesiumUniRule annotation
Metal bindingi244 – 2441Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi246 – 2461Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi249 – 2491PotassiumUniRule annotation
Metal bindingi250 – 2501MagnesiumUniRule annotation
Binding sitei452 – 4521FormyltetrahydrofolateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi225 – 2306GTPUniRule annotation
Nucleotide bindingi244 – 2507GTPUniRule annotation
Nucleotide bindingi269 – 2724GTPUniRule annotation
Nucleotide bindingi357 – 3593GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-16-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:thdFUniRule annotation, trmEUniRule annotation
Ordered Locus Names:BU016
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452tRNA modification GTPase MnmEPRO_0000188856Add
BLAST

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini215 – 376162TrmE-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0486.
HOGENOMiHOG000200714.
KOiK03650.
OMAiIMDEGIV.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57132-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRNETIVAQV TCPGKSAVGI LRISGFQTKI VAIKILGKIP LARFATYSNF
60 70 80 90 100
LDENNEILDK GISLWFPAPH SLTGEDVLEL QGHGSPLIMD LLIRRILSIN
110 120 130 140 150
NIRMAKPGEF SERAFLNGKM DLIQAESIDD LINSETELSA RASLNSLQGK
160 170 180 190 200
FSFFIKELMN LIIEFRINIE SNIDFSEEEI NINMQDIINI KFRELHDKFL
210 220 230 240 250
KLKATVLKGS LLREGKKIVI AGLPNSGKSS LLNILSYSDR AIVTDIPGTT
260 270 280 290 300
RDLLYEDISI NGVRCELIDT AGLRDTDDKV ERIGIMRAWE MIRKSDHVLF
310 320 330 340 350
VIDKTLSKSK QKKICDDFMK NILNNKTQVT FVLNKNDLIK DEFGIKEIDG
360 370 380 390 400
TAFISISART GEGVNILREH IIKIEKNINN ESVFIARRRH IKQLDLAYDE
410 420 430 440 450
FLTAENTWRE SENIELLAES LSIISKFLGE IIGQCTSEDL LNHIFSSFCI

GK
Length:452
Mass (Da):51,080
Last modified:December 1, 2000 - v1
Checksum:iCAF465790A479165
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12744.1.
RefSeqiNP_239858.2. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12744; BAB12744; BAB12744.
GeneIDi1109525.
KEGGibuc:BU016.
PATRICi21243536. VBIBucAph127364_0029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12744.1.
RefSeqiNP_239858.2. NC_002528.1.

3D structure databases

ProteinModelPortaliP57132.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12744; BAB12744; BAB12744.
GeneIDi1109525.
KEGGibuc:BU016.
PATRICi21243536. VBIBucAph127364_0029.

Phylogenomic databases

eggNOGiCOG0486.
HOGENOMiHOG000200714.
KOiK03650.
OMAiIMDEGIV.
OrthoDBiEOG6DC6K1.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-16-MONOMER.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiMNME_BUCAI
AccessioniPrimary (citable) accession number: P57132
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.