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P57132

- MNME_BUCAI

UniProt

P57132 - MNME_BUCAI

Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    • Comment

    Functioni

    Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

    Cofactori

    Binds 1 potassium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei22 – 221FormyltetrahydrofolateUniRule annotation
    Binding sitei79 – 791FormyltetrahydrofolateUniRule annotation
    Binding sitei119 – 1191FormyltetrahydrofolateUniRule annotation
    Metal bindingi225 – 2251PotassiumUniRule annotation
    Metal bindingi229 – 2291MagnesiumUniRule annotation
    Metal bindingi244 – 2441Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi246 – 2461Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi249 – 2491PotassiumUniRule annotation
    Metal bindingi250 – 2501MagnesiumUniRule annotation
    Binding sitei452 – 4521FormyltetrahydrofolateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi225 – 2306GTPUniRule annotation
    Nucleotide bindingi244 – 2507GTPUniRule annotation
    Nucleotide bindingi269 – 2724GTPUniRule annotation
    Nucleotide bindingi357 – 3593GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-16-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
    Gene namesi
    Name:mnmEUniRule annotation
    Synonyms:thdFUniRule annotation, trmEUniRule annotation
    Ordered Locus Names:BU016
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452tRNA modification GTPase MnmEPRO_0000188856Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57132.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini215 – 376162TrmE-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0486.
    HOGENOMiHOG000200714.
    KOiK03650.
    OMAiGVQSELY.
    OrthoDBiEOG6DC6K1.

    Family and domain databases

    Gene3Di1.20.120.430. 3 hits.
    3.30.1360.120. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00379. GTPase_MnmE.
    InterProiIPR018948. GTP-bd_TrmE_N.
    IPR006073. GTP_binding_domain.
    IPR004520. GTPase_MnmE.
    IPR025867. GTPase_MnmE_C_dom.
    IPR027368. MnmE_dom2.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view]
    PfamiPF12631. GTPase_Cys_C. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    PF10396. TrmE_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS51709. G_TRME. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57132-1 [UniParc]FASTAAdd to Basket

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    MRNETIVAQV TCPGKSAVGI LRISGFQTKI VAIKILGKIP LARFATYSNF    50
    LDENNEILDK GISLWFPAPH SLTGEDVLEL QGHGSPLIMD LLIRRILSIN 100
    NIRMAKPGEF SERAFLNGKM DLIQAESIDD LINSETELSA RASLNSLQGK 150
    FSFFIKELMN LIIEFRINIE SNIDFSEEEI NINMQDIINI KFRELHDKFL 200
    KLKATVLKGS LLREGKKIVI AGLPNSGKSS LLNILSYSDR AIVTDIPGTT 250
    RDLLYEDISI NGVRCELIDT AGLRDTDDKV ERIGIMRAWE MIRKSDHVLF 300
    VIDKTLSKSK QKKICDDFMK NILNNKTQVT FVLNKNDLIK DEFGIKEIDG 350
    TAFISISART GEGVNILREH IIKIEKNINN ESVFIARRRH IKQLDLAYDE 400
    FLTAENTWRE SENIELLAES LSIISKFLGE IIGQCTSEDL LNHIFSSFCI 450
    GK 452
    Length:452
    Mass (Da):51,080
    Last modified:December 1, 2000 - v1
    Checksum:iCAF465790A479165
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12744.1.
    RefSeqiNP_239858.2. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12744; BAB12744; BAB12744.
    GeneIDi1109525.
    KEGGibuc:BU016.
    PATRICi21243536. VBIBucAph127364_0029.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12744.1 .
    RefSeqi NP_239858.2. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57132.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12744 ; BAB12744 ; BAB12744 .
    GeneIDi 1109525.
    KEGGi buc:BU016.
    PATRICi 21243536. VBIBucAph127364_0029.

    Phylogenomic databases

    eggNOGi COG0486.
    HOGENOMi HOG000200714.
    KOi K03650.
    OMAi GVQSELY.
    OrthoDBi EOG6DC6K1.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-16-MONOMER.

    Family and domain databases

    Gene3Di 1.20.120.430. 3 hits.
    3.30.1360.120. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00379. GTPase_MnmE.
    InterProi IPR018948. GTP-bd_TrmE_N.
    IPR006073. GTP_binding_domain.
    IPR004520. GTPase_MnmE.
    IPR025867. GTPase_MnmE_C_dom.
    IPR027368. MnmE_dom2.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view ]
    Pfami PF12631. GTPase_Cys_C. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    PF10396. TrmE_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR00450. mnmE_trmE_thdF. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51709. G_TRME. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiMNME_BUCAI
    AccessioniPrimary (citable) accession number: P57132
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3