Reviewed, UniProtKB/Swiss-Prot P57130 (RNPA_BUCAI)

Names and origin

Protein names

Recommended name:
    Ribonuclease P protein component
      Short name=RNaseP protein
      Short name=RNase P protein
    EC=3.1.26.5
Alternative name(s):
    Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	BU014

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity.
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the rnpA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 115	115	Ribonuclease P protein component HAMAP MF_00227		PRO_0000198435

Sequences

	Sequence		Length	Mass (Da)
	P57130-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: BD2011A300351D58	FASTA	115	13,700
	10 20 30 40 50 60 MLNYFFKKQL RLSDSTSFQD VFNGSVKKKN TLEISILGRF NLLGHPRLGL SIPRKNIKHA 70 80 90 100 110 HNRNLIKRLV RETFRLLQYK LLSMDFVVIA KKNILFLNNT RIIDMLNSLW SNYYR

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12742.1.
RefSeq	NP_239856.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109523.
GenomeReviews	Gene locus BU014 in contig BA000003_GR.
KEGG	buc:BU014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57130.
OMA	PRIGLAI
Enzyme and pathway databases
BioCyc	BSP107806:BU014-MON.
Family and domain databases
HAMAP	MF_00227. [Tree]
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. IPR020539. RNase_P_CS. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_BUCAI

Accession

Primary (citable) accession number: P57130

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 24, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)