P57128 (DNAA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	BU012

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_0000114148
Regions
	Nucleotide binding	159 – 166	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P57128 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 1F5299F4C2751213	FASTA	454	52,931
	10 20 30 40 50 60 MSLCLWKQCL DRLQDELPNT EFSMWIRSLK AKLNNNILEI YAPNKFVLEW VKDKYLNHLK 70 80 90 100 110 120 KILQDYCGTN SPLIKFEIYQ IYKENKLKKN IENNNNNKNE KLIWSNIPKF KNLSYRSNIN 130 140 150 160 170 180 KRYNFQNFVE GKSNQLARSA AFQAARNPGN SYNPLFLYGA TGLGKTHLLH AIGNEILSYK 190 200 210 220 230 240 YDIKIIFMNS ECFVQDMVKA LKNNAIEKFK LYYRSVDALL IDDIQFFAHK ERSQEEFFHT 250 260 270 280 290 300 FNTLIEGNQQ IILTSDRYPK EINGVEDRLK SRFSWGLTIA IDPPEIETRV AILIKKADQN 310 320 330 340 350 360 NVILSNEVAF FIAKHLRSNV RELEGALNRV ILNSRFTHRA ITVDFAREAL QDILAVQEKI 370 380 390 400 410 420 ITIDNIQKTV AKYYKIKVSD LLSKKRSRSI ARPRQMAMAM AKKLTNHSLP EIGEAFSGRD 430 440 450 HTTVLHACCK IEQLRKENHD IKKDFLNLIR TLSK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12740.1.
RefSeq	NP_239854.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57128.
SMR	P57128. Positions 2-78, 361-453.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003267; EBBUCP00000003043; EBBUCG00000003267.
GeneID	1109521.
GenomeReviews	Gene locus BU012 in contig BA000003_GR.
KEGG	buc:BU012.
PATRIC	21243528. VBIBucAph127364_0025.
Phylogenomic databases
GeneTree	EBGT00050000008033.
HOGENOM	HBG695666.
OMA	DEVAFLI.
PhylomeDB	P57128.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	BSP107806:BU012-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_BUCAI

Accession

Primary (citable) accession number: P57128

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program