Reviewed, UniProtKB/Swiss-Prot P57127 (DPO3B_BUCAI)

Names and origin

Protein names

Recommended name:
    DNA polymerase III subunit beta
    EC=2.7.7.7

Gene names

Name:	dnaN
Ordered Locus Names:	BU011

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Contains a core (composed of alpha, epsilon, and theta chains) that can repair short gaps created by nuclease in duplex DNA. For efficient replication of the long, single-stranded templates, pol III requires the auxiliary chains beta, gamma, and delta By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	DNA polymerase III subunit beta		PRO_0000105426
Experimental info
	Sequence conflict	147	1	A → G in AAG33955. Ref.1
	Sequence conflict	152 – 153	2	RY → PI in AAG33955. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P57127-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 334F1880E8049604	FASTA	366	41,985
	10 20 30 40 50 60 MKFIINNNIL IKNLQKISRL LVKNTSLPIL DNVLINIKNG MLSLTGTNLE IELVAMIQLS 70 80 90 100 110 120 TEHISGTATI SGRKLLDICR NSLNSSNIEM QLNNNKMHII SGNSRYILTT LPYDSFPVHH 130 140 150 160 170 180 DFHHISEFFI PSDILKKMIE KIQFSMAKQD VRYYLNGILL EKTDRSLYAV ATDGYRLGIS 190 200 210 220 230 240 KFFLKENIIP FSIVIPRKGV IELYRLLNIP KQPIKVLVGK NNIRVHIEDL IFTTQLIEGQ 250 260 270 280 290 300 YPDYKSVLLE NKNNFITLNS KLLKQSLLRA AILSHEKFCG VEIHIRNGQF KVLSDNQEEE 310 320 330 340 350 360 IAEDRFNINY TGNTVKISIN VYYIIEILNS ITSENIFLFL NNANNSIQIE AENDASILYV VMLLKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vertical transmission of biosynthetic plasmids in aphid endosymbionts (Buchnera)." Wernegreen J.J., Moran N.A. J. Bacteriol. 183:785-790(2001) [PubMed: 11133977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF197895 Genomic DNA. Translation: AAG33955.1. BA000003 Genomic DNA. Translation: BAB12739.1.
RefSeq	NP_239853.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109520.
GenomeReviews	Gene locus BU011 in contig BA000003_GR.
KEGG	buc:BU011.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57127.
OMA	IGFNARY
Enzyme and pathway databases
BioCyc	BSP107806:BU011-MON.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. [Graphical view]
Gene3D	G3DSA:3.10.150.10. DNA_polIII_beta. 3 hits.
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3B_BUCAI

Accession

Primary (citable) accession number: P57127
Secondary accession number(s): Q9EVE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 24, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)