P57117 (MNMG_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	BU001

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00129
Cofactor	FAD By similarity. HAMAP MF_00129
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00129
Subcellular location	Cytoplasm By similarity HAMAP MF_00129.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP MF_00129		PRO_0000117072
Regions
	Nucleotide binding	13 – 18	6	FAD By similarity
	Nucleotide binding	273 – 287	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P57117 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 06E3745332BC077B	FASTA	628	70,307
	10 20 30 40 50 60 MFNLRNFDVI VVGAGHAGTE AAMASSRMGC KTLLLTQKIS DLGALSCNPA IGGIGKSHLV 70 80 90 100 110 120 KEIDALGGMM AKAIDYSGIQ FRILNSSKGP AVRSTRAQAD KILYHETVKK ILKKQNNLLI 130 140 150 160 170 180 LEAEVKDLIF KNYSVVGVLT QNEINFYSRS VVLAAGTFLG GKIHIGLKSY SAGRIGDKSA 190 200 210 220 230 240 IDLSVRLREL SLRVNRLKTG TPPRIDINTV NFNNLLIQNS DTPVPVFSFM GNVSHHPKQI 250 260 270 280 290 300 PCYLTHTNEK THEIIRKNLD KSPIYTGFLK GLGPRYCPSI EDKIVRFPDR KSHQVFLEPE 310 320 330 340 350 360 GLSSIKVYPN GISTSLPIEV QEQIVASIKG LEKSKIIRPG YAIEYDFFDP KDLNLTLESK 370 380 390 400 410 420 LIKGLFFAGQ INGTTGYEEA ASQGLLAGLN AALSSKNTEG WFPRRDQAYL GVLIDDLTTQ 430 440 450 460 470 480 GTEEPYRMFT SRAEYRLSLR EDNADLRLTE IGRKLGLVND SRWIRYNQKV LNIQTEMNRL 490 500 510 520 530 540 KKNKISPISP DADILKKLYN INLIKEISMS ELLKRPQIRY QDLQSLESFR TGIVDLEAIG 550 560 570 580 590 600 QIENEIKYAG YIKRQSEEIE RHLKNENTFL SSIYDYNKIR GLSSEVVKKL NDYKPISIGQ 610 620 ASRISGITPA AISILLIHLK KESYKHTL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12729.1.
RefSeq	NP_239843.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57117.
SMR	P57117. Positions 1-553.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002960; EBBUCP00000002736; EBBUCG00000002960.
GeneID	1109510.
GenomeReviews	Gene locus BU001 in contig BA000003_GR.
KEGG	buc:BU001.
PATRIC	21243506. VBIBucAph127364_0014.
Phylogenomic databases
GeneTree	EBGT00050000007979.
HOGENOM	HBG284774.
OMA	GFDRSPM.
PhylomeDB	P57117.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	BSP107806:BU001-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA. [Tree]
InterPro	IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K03495.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. GidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_BUCAI

Accession

Primary (citable) accession number: P57117

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program