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P57113

- MAAI_RAT

UniProt

P57113 - MAAI_RAT

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Protein

Maleylacetoacetate isomerase

Gene

Gstz1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides (By similarity). Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.By similarity1 Publication

Catalytic activityi

4-maleylacetoacetate = 4-fumarylacetoacetate.1 Publication
RX + glutathione = HX + R-S-glutathione.1 Publication

Cofactori

Glutathione. Required for the MAAI activity (By similarity).By similarity

Kineticsi

  1. KM=71.4 µM for dichloroacetic acid1 Publication
  2. KM=59.0 µM for glutathione1 Publication

Vmax=1334 nmol/min/mg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451GlutathioneBy similarity
Binding sitei59 – 591Glutathione; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei111 – 1111GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC
  2. maleylacetoacetate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutathione metabolic process Source: Ensembl
  2. L-phenylalanine catabolic process Source: UniProtKB-UniPathway
  3. tyrosine catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Enzyme and pathway databases

ReactomeiREACT_204731. Phenylalanine and tyrosine catabolism.
UniPathwayiUPA00139; UER00340.

Names & Taxonomyi

Protein namesi
Recommended name:
Maleylacetoacetate isomerase (EC:5.2.1.2)
Short name:
MAAI
Alternative name(s):
GSTZ1-1
Glutathione S-transferase zeta 1 (EC:2.5.1.18)
Gene namesi
Name:Gstz1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi1589363. Gstz1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Maleylacetoacetate isomerasePRO_0000186024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei57 – 571N6-succinyllysineBy similarity
Modified residuei136 – 1361PhosphothreonineBy similarity
Modified residuei177 – 1771N6-succinyllysineBy similarity
Modified residuei181 – 1811PhosphoserineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP57113.

Expressioni

Gene expression databases

GenevestigatoriP57113.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP57113.
SMRiP57113. Positions 4-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8784GST N-terminalAdd
BLAST
Domaini92 – 212121GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 196Glutathione bindingBy similarity
Regioni71 – 722Glutathione bindingBy similarity
Regioni115 – 1173Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006580.
HOVERGENiHBG001501.
InParanoidiP57113.
KOiK01800.
OMAiRAQVRMI.
OrthoDBiEOG7TF79P.
PhylomeDBiP57113.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57113-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAGKPVLYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFSEEF
60 70 80 90 100
QTLNPMKQVP ALKIDGITIG QSLAILEYLE ETRPIPRLLP QDPQKRAIVR
110 120 130 140 150
MISDLIASGI QPLQNLSVLK QVGQENQMPW AQKAITSGFN ALEKILQSTA
160 170 180 190 200
GKYCVGDEVS MADVCLAPQV ANAERFKVDL SPYPTISHIN KALLALEAFQ
210
VSHPCRQPDT PAELRT
Length:216
Mass (Da):23,961
Last modified:October 19, 2011 - v2
Checksum:i3E02041A8E3749A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ179410 mRNA. Translation: ACI32127.1.
CH473982 Genomic DNA. Translation: EDL81627.1.
BC158833 mRNA. Translation: AAI58834.1.
RefSeqiNP_001102915.1. NM_001109445.1.
UniGeneiRn.216913.

Genome annotation databases

EnsembliENSRNOT00000072215; ENSRNOP00000065390; ENSRNOG00000047708.
GeneIDi681913.
KEGGirno:681913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ179410 mRNA. Translation: ACI32127.1 .
CH473982 Genomic DNA. Translation: EDL81627.1 .
BC158833 mRNA. Translation: AAI58834.1 .
RefSeqi NP_001102915.1. NM_001109445.1.
UniGenei Rn.216913.

3D structure databases

ProteinModelPortali P57113.
SMRi P57113. Positions 4-214.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P57113.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000072215 ; ENSRNOP00000065390 ; ENSRNOG00000047708 .
GeneIDi 681913.
KEGGi rno:681913.

Organism-specific databases

CTDi 2954.
RGDi 1589363. Gstz1.

Phylogenomic databases

GeneTreei ENSGT00390000006580.
HOVERGENi HBG001501.
InParanoidi P57113.
KOi K01800.
OMAi RAQVRMI.
OrthoDBi EOG7TF79P.
PhylomeDBi P57113.

Enzyme and pathway databases

UniPathwayi UPA00139 ; UER00340 .
Reactomei REACT_204731. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

NextBioi 721886.

Gene expression databases

Genevestigatori P57113.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsi TIGR01262. maiA. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Microcystin-induced variations in transcription of GSTs in Wistar rat."
    Li G., Xie P.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  4. "Glutathione transferase zeta catalyses the oxygenation of the carcinogen dichloroacetic acid to glyoxylic acid."
    Tong Z., Board P.G., Anders M.W.
    Biochem. J. 331:371-374(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 131-158, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, BLOCKAGE OF N-TERMINUS.
    Strain: Fischer 344.
    Tissue: Liver.

Entry informationi

Entry nameiMAAI_RAT
AccessioniPrimary (citable) accession number: P57113
Secondary accession number(s): B0BNI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 19, 2011
Last modified: October 29, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3