Maleylacetoacetate isomerase - Rattus norvegicus (Rat)

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P57113 (MAAI_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Maleylacetoacetate isomerase
Short name=MAAI
EC=5.2.1.2

Alternative name(s):
GSTZ1-1
Glutathione S-transferase zeta 1
EC=2.5.1.18

Gene names

Name:

Gstz1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides By similarity. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. Ref.4
Catalytic activity	4-maleylacetoacetate = 4-fumarylacetoacetate. Ref.4 RX + glutathione = HX + R-S-glutathione. Ref.4
Cofactor	Glutathione. Required for the MAAI activity By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Ref.4.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the GST superfamily. Zeta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Biophysicochemical properties	Kinetic parameters: K_M=71.4 µM for dichloroacetic acid Ref.4 K_M=59.0 µM for glutathione V_max=1334 nmol/min/mg enzyme

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Cellular component	Cytoplasm
Molecular function	Isomerase Transferase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutathione metabolic process Inferred from electronic annotation. Source: Compara tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC maleylacetoacetate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 216

216

Maleylacetoacetate isomerase

PRO_0000186024

Regions

Domain

4 – 87

GST N-terminal

Domain

92 – 212

121

GST C-terminal

Region

14 – 19

Glutathione binding By similarity

Region

71 – 72

Glutathione binding By similarity

Region

115 – 117

Glutathione binding By similarity

Sites

Binding site

Glutathione By similarity

Binding site

Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Binding site

111

Glutathione By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P57113 [UniParc].

Last modified October 19, 2011. Version 2.
Checksum: 3E02041A8E3749A3
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FASTA

216

23,961

        10         20         30         40         50         60 
MQAGKPVLYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFSEEF QTLNPMKQVP 

        70         80         90        100        110        120 
ALKIDGITIG QSLAILEYLE ETRPIPRLLP QDPQKRAIVR MISDLIASGI QPLQNLSVLK 

       130        140        150        160        170        180 
QVGQENQMPW AQKAITSGFN ALEKILQSTA GKYCVGDEVS MADVCLAPQV ANAERFKVDL 

       190        200        210 
SPYPTISHIN KALLALEAFQ VSHPCRQPDT PAELRT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Microcystin-induced variations in transcription of GSTs in Wistar rat." Li G., Xie P. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[4]	"Glutathione transferase zeta catalyses the oxygenation of the carcinogen dichloroacetic acid to glyoxylic acid." Tong Z., Board P.G., Anders M.W. Biochem. J. 331:371-374(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 131-158, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, BLOCKAGE OF N-TERMINUS. Strain: Fischer 344. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FJ179410 mRNA. Translation: ACI32127.1. CH473982 Genomic DNA. Translation: EDL81627.1. BC158833 mRNA. Translation: AAI58834.1.
IPI	IPI00763872.
RefSeq	NP_001102915.1. NM_001109445.1.
UniGene	Rn.216913.
3D structure databases
ProteinModelPortal	P57113.
SMR	P57113. Positions 4-214.
ModBase	Search...
Proteomic databases
PRIDE	P57113.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000072215; ENSRNOP00000065390; ENSRNOG00000047708.
GeneID	681913.
KEGG	rno:681913.
Organism-specific databases
CTD	2954.
RGD	1589363. Gstz1.
Phylogenomic databases
GeneTree	ENSGT00390000006580.
HOVERGEN	HBG001501.
KO	K01800.
Enzyme and pathway databases
UniPathway	UPA00139; UER00340.
Gene expression databases
Genevestigator	P57113.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR005955. Mal_ac_isom. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01262. maiA. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	721886.

Entry information

Entry name

MAAI_RAT

Accession

Primary (citable) accession number: P57113
Secondary accession number(s): B0BNI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents