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P57113

- MAAI_RAT

UniProt

P57113 - MAAI_RAT

Protein

Maleylacetoacetate isomerase

Gene

Gstz1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides By similarity. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.By similarity1 Publication

    Catalytic activityi

    4-maleylacetoacetate = 4-fumarylacetoacetate.1 Publication
    RX + glutathione = HX + R-S-glutathione.1 Publication

    Cofactori

    Glutathione. Required for the MAAI activity By similarity.By similarity

    Kineticsi

    1. KM=71.4 µM for dichloroacetic acid1 Publication
    2. KM=59.0 µM for glutathione1 Publication

    Vmax=1334 nmol/min/mg enzyme1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451GlutathioneBy similarity
    Binding sitei59 – 591Glutathione; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei111 – 1111GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB-EC
    2. maleylacetoacetate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutathione metabolic process Source: Ensembl
    2. L-phenylalanine catabolic process Source: UniProtKB-UniPathway
    3. tyrosine catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Transferase

    Keywords - Biological processi

    Phenylalanine catabolism, Tyrosine catabolism

    Enzyme and pathway databases

    ReactomeiREACT_204731. Phenylalanine and tyrosine catabolism.
    UniPathwayiUPA00139; UER00340.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Maleylacetoacetate isomerase (EC:5.2.1.2)
    Short name:
    MAAI
    Alternative name(s):
    GSTZ1-1
    Glutathione S-transferase zeta 1 (EC:2.5.1.18)
    Gene namesi
    Name:Gstz1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi1589363. Gstz1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216Maleylacetoacetate isomerasePRO_0000186024Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei57 – 571N6-succinyllysineBy similarity
    Modified residuei136 – 1361PhosphothreonineBy similarity
    Modified residuei177 – 1771N6-succinyllysineBy similarity
    Modified residuei181 – 1811PhosphoserineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP57113.

    Expressioni

    Gene expression databases

    GenevestigatoriP57113.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57113.
    SMRiP57113. Positions 4-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8784GST N-terminalAdd
    BLAST
    Domaini92 – 212121GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 196Glutathione bindingBy similarity
    Regioni71 – 722Glutathione bindingBy similarity
    Regioni115 – 1173Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Zeta family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000006580.
    HOVERGENiHBG001501.
    KOiK01800.
    OMAiRAQVRMI.
    OrthoDBiEOG7TF79P.
    PhylomeDBiP57113.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR005955. Mal_ac_isom.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01262. maiA. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57113-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAGKPVLYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFSEEF    50
    QTLNPMKQVP ALKIDGITIG QSLAILEYLE ETRPIPRLLP QDPQKRAIVR 100
    MISDLIASGI QPLQNLSVLK QVGQENQMPW AQKAITSGFN ALEKILQSTA 150
    GKYCVGDEVS MADVCLAPQV ANAERFKVDL SPYPTISHIN KALLALEAFQ 200
    VSHPCRQPDT PAELRT 216
    Length:216
    Mass (Da):23,961
    Last modified:October 19, 2011 - v2
    Checksum:i3E02041A8E3749A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ179410 mRNA. Translation: ACI32127.1.
    CH473982 Genomic DNA. Translation: EDL81627.1.
    BC158833 mRNA. Translation: AAI58834.1.
    RefSeqiNP_001102915.1. NM_001109445.1.
    UniGeneiRn.216913.

    Genome annotation databases

    EnsembliENSRNOT00000072215; ENSRNOP00000065390; ENSRNOG00000047708.
    GeneIDi681913.
    KEGGirno:681913.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ179410 mRNA. Translation: ACI32127.1 .
    CH473982 Genomic DNA. Translation: EDL81627.1 .
    BC158833 mRNA. Translation: AAI58834.1 .
    RefSeqi NP_001102915.1. NM_001109445.1.
    UniGenei Rn.216913.

    3D structure databases

    ProteinModelPortali P57113.
    SMRi P57113. Positions 4-214.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P57113.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000072215 ; ENSRNOP00000065390 ; ENSRNOG00000047708 .
    GeneIDi 681913.
    KEGGi rno:681913.

    Organism-specific databases

    CTDi 2954.
    RGDi 1589363. Gstz1.

    Phylogenomic databases

    GeneTreei ENSGT00390000006580.
    HOVERGENi HBG001501.
    KOi K01800.
    OMAi RAQVRMI.
    OrthoDBi EOG7TF79P.
    PhylomeDBi P57113.

    Enzyme and pathway databases

    UniPathwayi UPA00139 ; UER00340 .
    Reactomei REACT_204731. Phenylalanine and tyrosine catabolism.

    Miscellaneous databases

    NextBioi 721886.

    Gene expression databases

    Genevestigatori P57113.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR005955. Mal_ac_isom.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01262. maiA. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Microcystin-induced variations in transcription of GSTs in Wistar rat."
      Li G., Xie P.
      Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    4. "Glutathione transferase zeta catalyses the oxygenation of the carcinogen dichloroacetic acid to glyoxylic acid."
      Tong Z., Board P.G., Anders M.W.
      Biochem. J. 331:371-374(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 131-158, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, BLOCKAGE OF N-TERMINUS.
      Strain: Fischer 344.
      Tissue: Liver.

    Entry informationi

    Entry nameiMAAI_RAT
    AccessioniPrimary (citable) accession number: P57113
    Secondary accession number(s): B0BNI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: October 19, 2011
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3