P57112 (STHA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1

Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	sth
Ordered Locus Names:	PA2991

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit.
Enzyme regulation	Strongly activated by NADPH and 2'-AMP and inhibited by NADP+. Ca2+ increases the activation and decreases the inhibition effect. HAMAP MF_00247
Subunit structure	Homooligomer; probably composed of four stacked rings of 7 or 8 monomers. Forms filamentous structures.
Subcellular location	Cytoplasm By similarity HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 464	463	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_0000068068
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57112 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 886CEAB5479997A9	FASTA	464	51,161
	10 20 30 40 50 60 MAVYNYDVVI LGTGPAGEGA AMNASKYGRK LAVVDSRRVV GGNCTHLGTI PSKALRHSVK 70 80 90 100 110 120 QIIEFNTNPM FRQIGEPRWF SFPDVLKSAD RVISKQVASR TGYYARNRID MFTGTASFVD 130 140 150 160 170 180 ERTVEVVTPS GAVERLVADQ FVIATGSRPY RPSDINFNHP RVYDSDTILS LSHTPRRLII 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VDLIDTRDQL LSFLDDEISD ALSYHLRNNN VLIRHNEEYE 250 260 270 280 290 300 RVEGLDNGVI LHLKSGKKIK ADALLWCNGR TGNTDKLGLE NVGIKVNSRG QIEVDENYRT 310 320 330 340 350 360 SVSNIFAAGD VIGWPSLASA AYDQGRSAAG NIVESDSWRF VNDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 ESELTAAKIP YEVGKAFFKG MARAQISNEP VGMLKILFHR ETLEILGVHC FGDQASEIVH 430 440 450 460 IGQAIMNQPG ELNTLKYFVN TTFNYPTMAE AYRVAAFDGL NRLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Pyridine nucleotide transhydrogenase from Pseudomonas aeruginosa: purification by affinity chromatography and physicochemical properties." Wermuth B., Kaplan N.O. Arch. Biochem. Biophys. 176:136-143(1976) [PubMed: 823872] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Pseudomonas aeruginosa transhydrogenase: affinity of substrates for the regulatory site and possible hysteretic behavior." Widmer F., Kaplan N.O. Biochem. Biophys. Res. Commun. 76:1287-1292(1977) [PubMed: 20086] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06379.1.
PIR	H83271.
RefSeq	NP_251681.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P57112.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878679.
GenomeReviews	Gene locus PA2991 in contig AE004091_GR.
KEGG	pae:PA2991.
PATRIC	19840539. VBIPseAer58763_3139.
Organism-specific databases
PseudoCAP	PA2991.
Phylogenomic databases
HOGENOM	HBG515043.
OMA	YSIDNAR.
ProtClustDB	PRK05249.
Enzyme and pathway databases
BioCyc	PAER208964:PA2991-MONOMER.
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR022962. STH. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00322.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

STHA_PSEAE

Accession

Primary (citable) accession number: P57112

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program