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P57110 (ATS8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 8

Short name=ADAM-TS 8
Short name=ADAM-TS8
Short name=ADAMTS-8
EC=3.4.24.-
Alternative name(s):
METH-2
Gene names
Name:Adamts8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length905 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has anti-angiogenic properties By similarity.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Expressed specifically in adult lung and heart and low expression during mouse development.

Domain

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 2 TSP type-1 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Propeptide29 – 228200 By similarity
PRO_0000029180
Chain229 – 905677A disintegrin and metalloproteinase with thrombospondin motifs 8
PRO_0000029181

Regions

Domain234 – 444211Peptidase M12B
Domain453 – 54189Disintegrin
Domain542 – 59756TSP type-1 1
Domain848 – 90457TSP type-1 2
Region706 – 847142Spacer
Compositional bias599 – 705107Cys-rich

Sites

Active site3791 By similarity
Metal binding3781Zinc; catalytic By similarity
Metal binding3821Zinc; catalytic By similarity
Metal binding3881Zinc; catalytic By similarity

Amino acid modifications

Glycosylation4151N-linked (GlcNAc...) Potential
Glycosylation4801N-linked (GlcNAc...) Potential
Glycosylation5061N-linked (GlcNAc...) Potential
Glycosylation6151N-linked (GlcNAc...) Potential
Disulfide bond309 ↔ 362 By similarity
Disulfide bond338 ↔ 344 By similarity
Disulfide bond356 ↔ 439 By similarity
Disulfide bond394 ↔ 423 By similarity
Disulfide bond478 ↔ 502 By similarity
Disulfide bond487 ↔ 523 By similarity
Disulfide bond517 ↔ 528 By similarity
Disulfide bond554 ↔ 591 By similarity
Disulfide bond558 ↔ 596 By similarity
Disulfide bond569 ↔ 581 By similarity

Sequences

Sequence LengthMass (Da)Tools
P57110 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 124D4132B33A0CAE

FASTA90598,880
        10         20         30         40         50         60 
MLRDPTTTGW PPLLLLLLQL PPPPLVCGAP AGPGTGAQAS ELVVPTRLPG SASELAFHLS 

        70         80         90        100        110        120 
AFGQGFVLRL APDASFLAPE FKIERLGGSS AAAGGEPGLR GCFFSGTVNG ERESLAAMSC 

       130        140        150        160        170        180 
VAGWSGSFLL AGEEFTIQPQ GAGDSLDQPH RLQRWGPGQR REDPGLAAAE VFPLPQGLEW 

       190        200        210        220        230        240 
EVEMGNGQGQ ERSDNEEDRK QDKEGLLKET EDSRKVPPPF GSKTRSKRFV SEARFVETLL 

       250        260        270        280        290        300 
VADASMAAFY GTDLQNHILT VMSMAARIYK HPSIRNSVNL VVVKVLIVEK ERWGPEVSDN 

       310        320        330        340        350        360 
GGLTLRNFCS WQRRFNKPSD RHPEHYDTAI LFTRQNFCGK GEQCDTLGMA DVGTICDPDK 

       370        380        390        400        410        420 
SCSVIKDEGL QAAYTLAHEL GHVLSMPHDD SKPCVRLFGP MGKYHMMAPF FIHVNKTLPW 

       430        440        450        460        470        480 
SPCSAVYLTE LLDDGHGDCL LDAPTSVLPL PTGLPGHSTL YELDQQCKQI FGPDFRHCPN 

       490        500        510        520        530        540 
TSVEDICVQL CARHRDSDEP ICHTKNGSLL WADGTPCGPG HLCLDGSCVL KEDVENPKAV 

       550        560        570        580        590        600 
VDGDWGPWRP WGQCSRTCGG GIQFSNRECD NPMPQNGGRF CLGERVKYQS CNTEECPPNG 

       610        620        630        640        650        660 
KSFREQQCEK YNAYNHTDLD GNFLQWVPKY SGVSPRDRCK LFCRARGRSE FKVFEAKVID 

       670        680        690        700        710        720 
GTLCGPDTLS ICVRGQCVKA GCDHVVNSPK KLDKCGVCGG KGTACRKISG SFTPFSYGYN 

       730        740        750        760        770        780 
DIVTIPAGAT NIDVKQRSHP GVRNDGSYLA LKTANGQYLL NGNLAISAIE QDILVKGTIL 

       790        800        810        820        830        840 
KYSGSMATLE RLQSFQALPE PLTVQLLTVS GEVFPPKVRY TFFVPNDMDF SVQNSKERAT 

       850        860        870        880        890        900 
TNIIQSLPSA EWVLGDWSEC PSTCRGSWQR RTVECRDPSG QASDTCDEAL KPEDAKPCGS 


QPCPL 

« Hide

References

[1]"ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11."
Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S.
Genomics 62:312-315(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF175282 mRNA. Translation: AAF25805.1.
RefSeqNP_038934.2. NM_013906.2.
UniGeneMm.100582.

3D structure databases

ProteinModelPortalP57110.
SMRP57110. Positions 234-825, 849-881.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM12.226.

PTM databases

PhosphoSiteP57110.

Proteomic databases

PRIDEP57110.

Protocols and materials databases

DNASU30806.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID30806.
KEGGmmu:30806.

Organism-specific databases

CTD11095.
MGIMGI:1353468. Adamts8.

Phylogenomic databases

eggNOGNOG285519.
HOGENOMHOG000004799.
HOVERGENHBG004313.
InParanoidP57110.
KOK08623.
PhylomeDBP57110.

Gene expression databases

GenevestigatorP57110.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
InterProIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR013277. Pept_M12B_ADAM-TS8.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PRINTSPR01861. ADAMTS8.
PR01857. ADAMTSFAMILY.
SMARTSM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMSSF82895. SSF82895. 2 hits.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio307226.
PROP57110.
SOURCESearch...

Entry information

Entry nameATS8_MOUSE
AccessionPrimary (citable) accession number: P57110
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot