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P57110

- ATS8_MOUSE

UniProt

P57110 - ATS8_MOUSE

Protein

A disintegrin and metalloproteinase with thrombospondin motifs 8

Gene

Adamts8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Has anti-angiogenic properties.By similarity

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi378 – 3781Zinc; catalyticBy similarity
    Active sitei379 – 3791PROSITE-ProRule annotation
    Metal bindingi382 – 3821Zinc; catalyticBy similarity
    Metal bindingi388 – 3881Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Heparin-binding, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM12.226.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 8 (EC:3.4.24.-)
    Short name:
    ADAM-TS 8
    Short name:
    ADAM-TS8
    Short name:
    ADAMTS-8
    Alternative name(s):
    METH-2
    Gene namesi
    Name:Adamts8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1353468. Adamts8.

    Subcellular locationi

    GO - Cellular componenti

    1. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Propeptidei29 – 228200By similarityPRO_0000029180Add
    BLAST
    Chaini229 – 905677A disintegrin and metalloproteinase with thrombospondin motifs 8PRO_0000029181Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi309 ↔ 362By similarity
    Disulfide bondi338 ↔ 344By similarity
    Disulfide bondi356 ↔ 439By similarity
    Disulfide bondi394 ↔ 423By similarity
    Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi478 ↔ 502By similarity
    Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi487 ↔ 523By similarity
    Glycosylationi506 – 5061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi517 ↔ 528By similarity
    Disulfide bondi554 ↔ 591By similarity
    Disulfide bondi558 ↔ 596By similarity
    Disulfide bondi569 ↔ 581By similarity
    Glycosylationi615 – 6151N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The precursor is cleaved by a furin endopeptidase.By similarity
    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiP57110.

    PTM databases

    PhosphoSiteiP57110.

    Expressioni

    Tissue specificityi

    Expressed specifically in adult lung and heart and low expression during mouse development.

    Gene expression databases

    GenevestigatoriP57110.

    Structurei

    3D structure databases

    ProteinModelPortaliP57110.
    SMRiP57110. Positions 234-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini234 – 444211Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini453 – 54189DisintegrinAdd
    BLAST
    Domaini542 – 59756TSP type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini848 – 90457TSP type-1 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni706 – 847142SpacerAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi599 – 705107Cys-richAdd
    BLAST

    Domaini

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

    Sequence similaritiesi

    Contains 1 disintegrin domain.Curated
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
    Contains 2 TSP type-1 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG285519.
    HOGENOMiHOG000004799.
    HOVERGENiHBG004313.
    InParanoidiP57110.
    KOiK08623.
    PhylomeDBiP57110.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    InterProiIPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR013277. Pept_M12B_ADAM-TS8.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR002870. Peptidase_M12B_N.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view]
    PfamiPF05986. ADAM_spacer1. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 1 hit.
    [Graphical view]
    PRINTSiPR01861. ADAMTS8.
    PR01857. ADAMTSFAMILY.
    SMARTiSM00209. TSP1. 2 hits.
    [Graphical view]
    SUPFAMiSSF82895. SSF82895. 2 hits.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS50092. TSP1. 2 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P57110-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRDPTTTGW PPLLLLLLQL PPPPLVCGAP AGPGTGAQAS ELVVPTRLPG    50
    SASELAFHLS AFGQGFVLRL APDASFLAPE FKIERLGGSS AAAGGEPGLR 100
    GCFFSGTVNG ERESLAAMSC VAGWSGSFLL AGEEFTIQPQ GAGDSLDQPH 150
    RLQRWGPGQR REDPGLAAAE VFPLPQGLEW EVEMGNGQGQ ERSDNEEDRK 200
    QDKEGLLKET EDSRKVPPPF GSKTRSKRFV SEARFVETLL VADASMAAFY 250
    GTDLQNHILT VMSMAARIYK HPSIRNSVNL VVVKVLIVEK ERWGPEVSDN 300
    GGLTLRNFCS WQRRFNKPSD RHPEHYDTAI LFTRQNFCGK GEQCDTLGMA 350
    DVGTICDPDK SCSVIKDEGL QAAYTLAHEL GHVLSMPHDD SKPCVRLFGP 400
    MGKYHMMAPF FIHVNKTLPW SPCSAVYLTE LLDDGHGDCL LDAPTSVLPL 450
    PTGLPGHSTL YELDQQCKQI FGPDFRHCPN TSVEDICVQL CARHRDSDEP 500
    ICHTKNGSLL WADGTPCGPG HLCLDGSCVL KEDVENPKAV VDGDWGPWRP 550
    WGQCSRTCGG GIQFSNRECD NPMPQNGGRF CLGERVKYQS CNTEECPPNG 600
    KSFREQQCEK YNAYNHTDLD GNFLQWVPKY SGVSPRDRCK LFCRARGRSE 650
    FKVFEAKVID GTLCGPDTLS ICVRGQCVKA GCDHVVNSPK KLDKCGVCGG 700
    KGTACRKISG SFTPFSYGYN DIVTIPAGAT NIDVKQRSHP GVRNDGSYLA 750
    LKTANGQYLL NGNLAISAIE QDILVKGTIL KYSGSMATLE RLQSFQALPE 800
    PLTVQLLTVS GEVFPPKVRY TFFVPNDMDF SVQNSKERAT TNIIQSLPSA 850
    EWVLGDWSEC PSTCRGSWQR RTVECRDPSG QASDTCDEAL KPEDAKPCGS 900
    QPCPL 905
    Length:905
    Mass (Da):98,880
    Last modified:December 1, 2000 - v1
    Checksum:i124D4132B33A0CAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF175282 mRNA. Translation: AAF25805.1.
    CCDSiCCDS22946.1.
    RefSeqiNP_038934.2. NM_013906.2.
    UniGeneiMm.100582.

    Genome annotation databases

    GeneIDi30806.
    KEGGimmu:30806.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF175282 mRNA. Translation: AAF25805.1 .
    CCDSi CCDS22946.1.
    RefSeqi NP_038934.2. NM_013906.2.
    UniGenei Mm.100582.

    3D structure databases

    ProteinModelPortali P57110.
    SMRi P57110. Positions 234-825.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.226.

    PTM databases

    PhosphoSitei P57110.

    Proteomic databases

    PRIDEi P57110.

    Protocols and materials databases

    DNASUi 30806.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 30806.
    KEGGi mmu:30806.

    Organism-specific databases

    CTDi 11095.
    MGIi MGI:1353468. Adamts8.

    Phylogenomic databases

    eggNOGi NOG285519.
    HOGENOMi HOG000004799.
    HOVERGENi HBG004313.
    InParanoidi P57110.
    KOi K08623.
    PhylomeDBi P57110.

    Miscellaneous databases

    NextBioi 307226.
    PROi P57110.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P57110.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    InterProi IPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR013277. Pept_M12B_ADAM-TS8.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR002870. Peptidase_M12B_N.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view ]
    Pfami PF05986. ADAM_spacer1. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01861. ADAMTS8.
    PR01857. ADAMTSFAMILY.
    SMARTi SM00209. TSP1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF82895. SSF82895. 2 hits.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS50092. TSP1. 2 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11."
      Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S.
      Genomics 62:312-315(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiATS8_MOUSE
    AccessioniPrimary (citable) accession number: P57110
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3