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P57110

- ATS8_MOUSE

UniProt

P57110 - ATS8_MOUSE

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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 8

Gene
Adamts8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Has anti-angiogenic properties By similarity.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi378 – 3781Zinc; catalytic By similarity
Active sitei379 – 3791 By similarity
Metal bindingi382 – 3821Zinc; catalytic By similarity
Metal bindingi388 – 3881Zinc; catalytic By similarity

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Heparin-binding, Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.226.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 8 (EC:3.4.24.-)
Short name:
ADAM-TS 8
Short name:
ADAM-TS8
Short name:
ADAMTS-8
Alternative name(s):
METH-2
Gene namesi
Name:Adamts8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1353468. Adamts8.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed predictionAdd
BLAST
Propeptidei29 – 228200 By similarityPRO_0000029180Add
BLAST
Chaini229 – 905677A disintegrin and metalloproteinase with thrombospondin motifs 8PRO_0000029181Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi309 ↔ 362 By similarity
Disulfide bondi338 ↔ 344 By similarity
Disulfide bondi356 ↔ 439 By similarity
Disulfide bondi394 ↔ 423 By similarity
Glycosylationi415 – 4151N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi478 ↔ 502 By similarity
Glycosylationi480 – 4801N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi487 ↔ 523 By similarity
Glycosylationi506 – 5061N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi517 ↔ 528 By similarity
Disulfide bondi554 ↔ 591 By similarity
Disulfide bondi558 ↔ 596 By similarity
Disulfide bondi569 ↔ 581 By similarity
Glycosylationi615 – 6151N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase By similarity.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP57110.

PTM databases

PhosphoSiteiP57110.

Expressioni

Tissue specificityi

Expressed specifically in adult lung and heart and low expression during mouse development.

Gene expression databases

GenevestigatoriP57110.

Structurei

3D structure databases

ProteinModelPortaliP57110.
SMRiP57110. Positions 234-825.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini234 – 444211Peptidase M12BAdd
BLAST
Domaini453 – 54189DisintegrinAdd
BLAST
Domaini542 – 59756TSP type-1 1Add
BLAST
Domaini848 – 90457TSP type-1 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni706 – 847142SpacerAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi599 – 705107Cys-richAdd
BLAST

Domaini

The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

Sequence similaritiesi

Contains 1 disintegrin domain.
Contains 2 TSP type-1 domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG285519.
HOGENOMiHOG000004799.
HOVERGENiHBG004313.
InParanoidiP57110.
KOiK08623.
PhylomeDBiP57110.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR013277. Pept_M12B_ADAM-TS8.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PRINTSiPR01861. ADAMTS8.
PR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 2 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57110-1 [UniParc]FASTAAdd to Basket

« Hide

MLRDPTTTGW PPLLLLLLQL PPPPLVCGAP AGPGTGAQAS ELVVPTRLPG    50
SASELAFHLS AFGQGFVLRL APDASFLAPE FKIERLGGSS AAAGGEPGLR 100
GCFFSGTVNG ERESLAAMSC VAGWSGSFLL AGEEFTIQPQ GAGDSLDQPH 150
RLQRWGPGQR REDPGLAAAE VFPLPQGLEW EVEMGNGQGQ ERSDNEEDRK 200
QDKEGLLKET EDSRKVPPPF GSKTRSKRFV SEARFVETLL VADASMAAFY 250
GTDLQNHILT VMSMAARIYK HPSIRNSVNL VVVKVLIVEK ERWGPEVSDN 300
GGLTLRNFCS WQRRFNKPSD RHPEHYDTAI LFTRQNFCGK GEQCDTLGMA 350
DVGTICDPDK SCSVIKDEGL QAAYTLAHEL GHVLSMPHDD SKPCVRLFGP 400
MGKYHMMAPF FIHVNKTLPW SPCSAVYLTE LLDDGHGDCL LDAPTSVLPL 450
PTGLPGHSTL YELDQQCKQI FGPDFRHCPN TSVEDICVQL CARHRDSDEP 500
ICHTKNGSLL WADGTPCGPG HLCLDGSCVL KEDVENPKAV VDGDWGPWRP 550
WGQCSRTCGG GIQFSNRECD NPMPQNGGRF CLGERVKYQS CNTEECPPNG 600
KSFREQQCEK YNAYNHTDLD GNFLQWVPKY SGVSPRDRCK LFCRARGRSE 650
FKVFEAKVID GTLCGPDTLS ICVRGQCVKA GCDHVVNSPK KLDKCGVCGG 700
KGTACRKISG SFTPFSYGYN DIVTIPAGAT NIDVKQRSHP GVRNDGSYLA 750
LKTANGQYLL NGNLAISAIE QDILVKGTIL KYSGSMATLE RLQSFQALPE 800
PLTVQLLTVS GEVFPPKVRY TFFVPNDMDF SVQNSKERAT TNIIQSLPSA 850
EWVLGDWSEC PSTCRGSWQR RTVECRDPSG QASDTCDEAL KPEDAKPCGS 900
QPCPL 905
Length:905
Mass (Da):98,880
Last modified:December 1, 2000 - v1
Checksum:i124D4132B33A0CAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF175282 mRNA. Translation: AAF25805.1.
CCDSiCCDS22946.1.
RefSeqiNP_038934.2. NM_013906.2.
UniGeneiMm.100582.

Genome annotation databases

GeneIDi30806.
KEGGimmu:30806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF175282 mRNA. Translation: AAF25805.1 .
CCDSi CCDS22946.1.
RefSeqi NP_038934.2. NM_013906.2.
UniGenei Mm.100582.

3D structure databases

ProteinModelPortali P57110.
SMRi P57110. Positions 234-825.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.226.

PTM databases

PhosphoSitei P57110.

Proteomic databases

PRIDEi P57110.

Protocols and materials databases

DNASUi 30806.
Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 30806.
KEGGi mmu:30806.

Organism-specific databases

CTDi 11095.
MGIi MGI:1353468. Adamts8.

Phylogenomic databases

eggNOGi NOG285519.
HOGENOMi HOG000004799.
HOVERGENi HBG004313.
InParanoidi P57110.
KOi K08623.
PhylomeDBi P57110.

Miscellaneous databases

NextBioi 307226.
PROi P57110.
SOURCEi Search...

Gene expression databases

Genevestigatori P57110.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
InterProi IPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR013277. Pept_M12B_ADAM-TS8.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view ]
Pfami PF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view ]
PRINTSi PR01861. ADAMTS8.
PR01857. ADAMTSFAMILY.
SMARTi SM00209. TSP1. 2 hits.
[Graphical view ]
SUPFAMi SSF82895. SSF82895. 2 hits.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS50092. TSP1. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11."
    Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S.
    Genomics 62:312-315(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiATS8_MOUSE
AccessioniPrimary (citable) accession number: P57110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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