P57110 (ATS8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 8
Short name=ADAM-TS 8
Short name=ADAM-TS8
Short name=ADAMTS-8
EC=3.4.24.-

Alternative name(s):
METH-2

Gene names

Name:

Adamts8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has anti-angiogenic properties By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed specifically in adult lung and heart and low expression during mouse development.
Domain	The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 2 TSP type-1 domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Repeat Signal
Ligand	Heparin-binding Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Propeptide	29 – 228	200	By similarity		PRO_0000029180
	Chain	229 – 905	677	A disintegrin and metalloproteinase with thrombospondin motifs 8		PRO_0000029181
Regions
	Domain	234 – 444	211	Peptidase M12B
	Domain	453 – 541	89	Disintegrin
	Domain	542 – 597	56	TSP type-1 1
	Domain	848 – 904	57	TSP type-1 2
	Region	706 – 847	142	Spacer
	Compositional bias	599 – 705	107	Cys-rich
Sites
	Active site	379	1	By similarity
	Metal binding	378	1	Zinc; catalytic By similarity
	Metal binding	382	1	Zinc; catalytic By similarity
	Metal binding	388	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	415	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential
	Glycosylation	506	1	N-linked (GlcNAc...) Potential
	Glycosylation	615	1	N-linked (GlcNAc...) Potential
	Disulfide bond	356 ↔ 439		By similarity
	Disulfide bond	394 ↔ 423		By similarity
	Disulfide bond	554 ↔ 591		By similarity
	Disulfide bond	558 ↔ 596		By similarity
	Disulfide bond	569 ↔ 581		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57110 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 124D4132B33A0CAE	FASTA	905	98,880
	10 20 30 40 50 60 MLRDPTTTGW PPLLLLLLQL PPPPLVCGAP AGPGTGAQAS ELVVPTRLPG SASELAFHLS 70 80 90 100 110 120 AFGQGFVLRL APDASFLAPE FKIERLGGSS AAAGGEPGLR GCFFSGTVNG ERESLAAMSC 130 140 150 160 170 180 VAGWSGSFLL AGEEFTIQPQ GAGDSLDQPH RLQRWGPGQR REDPGLAAAE VFPLPQGLEW 190 200 210 220 230 240 EVEMGNGQGQ ERSDNEEDRK QDKEGLLKET EDSRKVPPPF GSKTRSKRFV SEARFVETLL 250 260 270 280 290 300 VADASMAAFY GTDLQNHILT VMSMAARIYK HPSIRNSVNL VVVKVLIVEK ERWGPEVSDN 310 320 330 340 350 360 GGLTLRNFCS WQRRFNKPSD RHPEHYDTAI LFTRQNFCGK GEQCDTLGMA DVGTICDPDK 370 380 390 400 410 420 SCSVIKDEGL QAAYTLAHEL GHVLSMPHDD SKPCVRLFGP MGKYHMMAPF FIHVNKTLPW 430 440 450 460 470 480 SPCSAVYLTE LLDDGHGDCL LDAPTSVLPL PTGLPGHSTL YELDQQCKQI FGPDFRHCPN 490 500 510 520 530 540 TSVEDICVQL CARHRDSDEP ICHTKNGSLL WADGTPCGPG HLCLDGSCVL KEDVENPKAV 550 560 570 580 590 600 VDGDWGPWRP WGQCSRTCGG GIQFSNRECD NPMPQNGGRF CLGERVKYQS CNTEECPPNG 610 620 630 640 650 660 KSFREQQCEK YNAYNHTDLD GNFLQWVPKY SGVSPRDRCK LFCRARGRSE FKVFEAKVID 670 680 690 700 710 720 GTLCGPDTLS ICVRGQCVKA GCDHVVNSPK KLDKCGVCGG KGTACRKISG SFTPFSYGYN 730 740 750 760 770 780 DIVTIPAGAT NIDVKQRSHP GVRNDGSYLA LKTANGQYLL NGNLAISAIE QDILVKGTIL 790 800 810 820 830 840 KYSGSMATLE RLQSFQALPE PLTVQLLTVS GEVFPPKVRY TFFVPNDMDF SVQNSKERAT 850 860 870 880 890 900 TNIIQSLPSA EWVLGDWSEC PSTCRGSWQR RTVECRDPSG QASDTCDEAL KPEDAKPCGS QPCPL

References

[1]	"ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11." Georgiadis K.E., Hirohata S., Seldin M.F., Apte S.S. Genomics 62:312-315(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175282 mRNA. Translation: AAF25805.1.
IPI	IPI00114024.
RefSeq	NP_038934.2. NM_013906.2.
UniGene	Mm.100582.
3D structure databases
ProteinModelPortal	P57110.
SMR	P57110. Positions 234-825.
ModBase	Search...
Protein family/group databases
MEROPS	M12.226.
PTM databases
PhosphoSite	P57110.
Proteomic databases
PRIDE	P57110.
Protocols and materials databases
DNASU	30806.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	30806.
KEGG	mmu:30806.
UCSC	uc009ora.1. mouse.
Organism-specific databases
CTD	11095.
MGI	MGI:1353468. Adamts8.
Phylogenomic databases
eggNOG	NOG285519.
HOGENOM	HOG000004799.
HOVERGEN	HBG004313.
InParanoid	P57110.
KO	K08623.
OrthoDB	EOG4WQ125.
Gene expression databases
Genevestigator	P57110.
Family and domain databases
Gene3D	3.40.390.10. 1 hit.
InterPro	IPR010294. ADAM_spacer1. IPR024079. MetalloPept_cat_dom. IPR013277. Pept_M12B_ADAM-TS8. IPR001590. Peptidase_M12B. IPR013273. Peptidase_M12B_ADAM-TS. IPR002870. Peptidase_M12B_N. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Pfam	PF05986. ADAM_spacer1. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. PF00090. TSP_1. 1 hit. [Graphical view]
PRINTS	PR01861. ADAMTS8. PR01857. ADAMTSFAMILY.
SMART	SM00209. TSP1. 2 hits. [Graphical view]
SUPFAM	SSF82895. TSP1. 2 hits.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. False negative. PS50214. DISINTEGRIN_2. False negative. PS50092. TSP1. 2 hits. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307226.
SOURCE	Search...

Entry information

Entry name

ATS8_MOUSE

Accession

Primary (citable) accession number: P57110

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program