Malate dehydrogenase, cytoplasmic 2 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot P57106 (MDHC2_ARATH)

Last modified June 16, 2009. Version 71. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Malate dehydrogenase, cytoplasmic 2
EC=1.1.1.37

Gene names

Ordered Locus Names:	At5g43330
ORF Names:	MWF20.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

332

Malate dehydrogenase, cytoplasmic 2

PRO_0000113413

Regions

Nucleotide binding

7

NAD By similarity

Nucleotide binding

3

NAD By similarity

Sites

Active site

1

Proton acceptor By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P57106-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 76BF976943179FA7
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332

35,675

        10         20         30         40         50         60 
MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPFAAEA LNGVKMELVD 

        70         80         90        100        110        120 
AAFPLLKGVV ATTDAVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEKH 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQVSE RLSVPVSDVK 

       190        200        210        220        230        240 
NVIIWGNHSS TQYPDVNHAT VKTSVGEKPV RELVKNDEWL NGEFISTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
LSSALSAASS ACDHIRDWVV GTPEGTFVSM GVYSDGSYNV PAGLIYSFPV TCRNGEWTIV 

       310        320        330 
QGLPIDDASR KKMDLTAEEL KEEKDLAYSC LS

References

[1]

"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

[2]

"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.

expand/collapse author list

Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases
	AB025638 Genomic DNA. Translation: BAA97412.1. AY072137 mRNA. Translation: AAL59959.1. AY091220 mRNA. Translation: AAM14159.1.
IPI	IPI00543463.
RefSeq	NP_199147.1.
UniGene	At.27775
3D structure databases
HSSP	HSSP built from PDB template 4MDH based on UniProtKB P11708.
ModBase	Search...
Proteomic databases
PRIDE	P57106.
Genome annotation databases
GeneID	834351.
GenomeReviews	Gene locus AT5G43330 in contig BA000015_GR.
KEGG	ath:AT5G43330.
NMPDR	fig\|3702.1.peg.26071.
Organism-specific databases
TAIR	At5g43330.
Phylogenomic databases
OMA	P57106. CGVPATH.
Enzyme and pathway databases
BRENDA	1.1.1.37. 302.
Gene expression databases
ArrayExpress	P57106.
GermOnline	AT5G43330. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR011274. Malate_DH_NAD-dep_euk. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHC2_ARATH

Accession

Primary (citable) accession number: P57106
Secondary accession number(s): Q9LSX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents