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Protein

Malate dehydrogenase 2, cytoplasmic

Gene

MDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43NADBy similarity1
Binding sitei93SubstrateBy similarity1
Binding sitei99SubstrateBy similarity1
Binding sitei106NADBy similarity1
Binding sitei113NADBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei163SubstrateBy similarity1
Active sitei188Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADBy similarity7
Nucleotide bindingi130 – 132NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

ReactomeiR-ATH-70263 Gluconeogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 2, cytoplasmicCurated (EC:1.1.1.37)
Alternative name(s):
Cytosolic NAD-dependent malate dehydrogenase 2Curated
Short name:
cNAD-MDH21 Publication
Cytosolic malate dehydrogenase 21 Publication
Short name:
Cytosolic MDH2Curated
Gene namesi
Name:MDH2
Ordered Locus Names:At5g43330
ORF Names:MWF20.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G43330
TAIRilocus:2176441 AT5G43330

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134131 – 332Malate dehydrogenase 2, cytoplasmicAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP57106
PRIDEiP57106

PTM databases

iPTMnetiP57106

Expressioni

Tissue specificityi

Expressed in rosette leaves at low levels.1 Publication

Gene expression databases

ExpressionAtlasiP57106 baseline and differential
GenevisibleiP57106 AT

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with 14-3-3-like proteins GRF1 GRF3 and GRF8 (PubMed:22104211).By similarity1 Publication

Protein-protein interaction databases

BioGridi19601, 3 interactors
STRINGi3702.AT5G43330.1

Structurei

3D structure databases

ProteinModelPortaliP57106
SMRiP57106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiKOG1496 Eukaryota
COG0039 LUCA
HOGENOMiHOG000220953
InParanoidiP57106
KOiK00025
OMAiRPRTKGM
OrthoDBiEOG09360G1U
PhylomeDBiP57106

Family and domain databases

CDDicd01336 MDH_cytoplasmic_cytosolic, 1 hit
Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR011274 Malate_DH_NAD-dep_euk
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit
TIGR01758 MDH_euk_cyt, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

P57106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPFAAEA
60 70 80 90 100
LNGVKMELVD AAFPLLKGVV ATTDAVEACT GVNVAVMVGG FPRKEGMERK
110 120 130 140 150
DVMSKNVSIY KSQASALEKH AAPNCKVLVV ANPANTNALI LKEFAPSIPE
160 170 180 190 200
KNITCLTRLD HNRALGQVSE RLSVPVSDVK NVIIWGNHSS TQYPDVNHAT
210 220 230 240 250
VKTSVGEKPV RELVKNDEWL NGEFISTVQQ RGAAIIKARK LSSALSAASS
260 270 280 290 300
ACDHIRDWVV GTPEGTFVSM GVYSDGSYNV PAGLIYSFPV TCRNGEWTIV
310 320 330
QGLPIDDASR KKMDLTAEEL KEEKDLAYSC LS
Length:332
Mass (Da):35,675
Last modified:December 1, 2000 - v1
Checksum:i76BF976943179FA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4E → K in AAM65532 (Ref. 4) Curated1
Sequence conflicti205V → S in AAM65532 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025638 Genomic DNA Translation: BAA97412.1
CP002688 Genomic DNA Translation: AED94946.1
AY072137 mRNA Translation: AAL59959.1
AY091220 mRNA Translation: AAM14159.1
AY087986 mRNA Translation: AAM65532.1
RefSeqiNP_199147.1, NM_123699.4
UniGeneiAt.27775

Genome annotation databases

EnsemblPlantsiAT5G43330.1; AT5G43330.1; AT5G43330
GeneIDi834351
GrameneiAT5G43330.1; AT5G43330.1; AT5G43330
KEGGiath:AT5G43330

Similar proteinsi

Entry informationi

Entry nameiMDHC2_ARATH
AccessioniPrimary (citable) accession number: P57106
Secondary accession number(s): Q8LA78, Q9LSX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 25, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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