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Protein

Malate dehydrogenase, cytoplasmic 2

Gene

MDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstratePROSITE-ProRule annotation
Binding sitei99 – 991SubstratePROSITE-ProRule annotation
Binding sitei106 – 1061NADBy similarity
Binding sitei113 – 1131NADBy similarity
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei163 – 1631SubstratePROSITE-ProRule annotation
Active sitei188 – 1881Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADBy similarity
Nucleotide bindingi130 – 1323NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_298151. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic 2 (EC:1.1.1.37)
Gene namesi
Name:MDH2
Ordered Locus Names:At5g43330
ORF Names:MWF20.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G43330.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Malate dehydrogenase, cytoplasmic 2PRO_0000113413Add
BLAST

Proteomic databases

PaxDbiP57106.
PRIDEiP57106.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with 14-3-3-like proteins GRF1 GRF3 and GRF8 (PubMed:22104211).By similarity1 Publication

Protein-protein interaction databases

BioGridi19601. 3 interactions.
MINTiMINT-8062783.
STRINGi3702.AT5G43330.1.

Structurei

3D structure databases

ProteinModelPortaliP57106.
SMRiP57106. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
InParanoidiP57106.
KOiK00025.
OMAiNDEWLNG.
PhylomeDBiP57106.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPFAAEA
60 70 80 90 100
LNGVKMELVD AAFPLLKGVV ATTDAVEACT GVNVAVMVGG FPRKEGMERK
110 120 130 140 150
DVMSKNVSIY KSQASALEKH AAPNCKVLVV ANPANTNALI LKEFAPSIPE
160 170 180 190 200
KNITCLTRLD HNRALGQVSE RLSVPVSDVK NVIIWGNHSS TQYPDVNHAT
210 220 230 240 250
VKTSVGEKPV RELVKNDEWL NGEFISTVQQ RGAAIIKARK LSSALSAASS
260 270 280 290 300
ACDHIRDWVV GTPEGTFVSM GVYSDGSYNV PAGLIYSFPV TCRNGEWTIV
310 320 330
QGLPIDDASR KKMDLTAEEL KEEKDLAYSC LS
Length:332
Mass (Da):35,675
Last modified:December 1, 2000 - v1
Checksum:i76BF976943179FA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025638 Genomic DNA. Translation: BAA97412.1.
CP002688 Genomic DNA. Translation: AED94946.1.
AY072137 mRNA. Translation: AAL59959.1.
AY091220 mRNA. Translation: AAM14159.1.
RefSeqiNP_199147.1. NM_123699.3.
UniGeneiAt.27775.

Genome annotation databases

EnsemblPlantsiAT5G43330.1; AT5G43330.1; AT5G43330.
GeneIDi834351.
KEGGiath:AT5G43330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025638 Genomic DNA. Translation: BAA97412.1.
CP002688 Genomic DNA. Translation: AED94946.1.
AY072137 mRNA. Translation: AAL59959.1.
AY091220 mRNA. Translation: AAM14159.1.
RefSeqiNP_199147.1. NM_123699.3.
UniGeneiAt.27775.

3D structure databases

ProteinModelPortaliP57106.
SMRiP57106. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19601. 3 interactions.
MINTiMINT-8062783.
STRINGi3702.AT5G43330.1.

Proteomic databases

PaxDbiP57106.
PRIDEiP57106.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G43330.1; AT5G43330.1; AT5G43330.
GeneIDi834351.
KEGGiath:AT5G43330.

Organism-specific databases

TAIRiAT5G43330.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
InParanoidiP57106.
KOiK00025.
OMAiNDEWLNG.
PhylomeDBiP57106.

Enzyme and pathway databases

ReactomeiREACT_298151. Gluconeogenesis.

Miscellaneous databases

PROiP57106.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Determining novel functions of Arabidopsis 14-3-3 proteins in central metabolic processes."
    Diaz C., Kusano M., Sulpice R., Araki M., Redestig H., Saito K., Stitt M., Shin R.
    BMC Syst. Biol. 5:192-192(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRF1; GRF3 AND GRF8.

Entry informationi

Entry nameiMDHC2_ARATH
AccessioniPrimary (citable) accession number: P57106
Secondary accession number(s): Q9LSX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 22, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.