P57102 (HAND2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heart- and neural crest derivatives-expressed protein 2

Alternative name(s):
Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2
Short name=dHAND

Gene names

Name:	hand2
Synonyms:	dhand

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for myocardial and pectoral fin differentiation, patterning and morphogenesis. Ref.2
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein.
Subcellular location	Nucleus By similarity.
Developmental stage	Expression first detected at 8 hours post fertilization (hpf) in a circumferential pattern in the lateral mesoderm. At 12 hpf, still expressed in the anterior and posterior lateral plate mesoderm which is partly detached from the yolk at this stage. By 18-20 hpf, expressed in the cardiac ring and in a layer of lateral mesoderm surrounding the cardiac region, as well as in the posterior lateral mesoderm. At 24 hpf, expressed throughout the nascent cardiac tube and in bilaterally symmetric clusters of ventrolateral cells that condense to form branchial arch mesoderm which gives rise to jaw and pharyngeal bones. Expression continues bilaterally in the posterior lateral mesoderm. At 36-48 hpf, expressed in defined branchial arch mesoderm populations. At 48 hpf, strong expression throughout the heart tube.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cardioblast differentiation Inferred from mutant phenotype. Source: ZFIN cardiogenic plate morphogenesis Inferred from mutant phenotype. Source: ZFIN determination of intestine left/right asymmetry Inferred from mutant phenotype. Source: ZFIN embryonic heart tube formation Inferred from mutant phenotype. Source: ZFIN embryonic pectoral fin morphogenesis Inferred from mutant phenotype. Source: ZFIN embryonic viscerocranium morphogenesis Inferred from mutant phenotype. Source: ZFIN heart contraction Inferred from mutant phenotype. Source: ZFIN polarized epithelial cell differentiation Inferred from mutant phenotype. Source: ZFIN regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW thyroid gland development Inferred from mutant phenotype. Source: ZFIN transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Heart- and neural crest derivatives-expressed protein 2		PRO_0000127194
Regions
	Domain	103 – 146	44	Helix-loop-helix motif
	DNA binding	90 – 102	13	Basic motif
	Compositional bias	21 – 26	6	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	P57102 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: F0E63A9329AC6E06	FASTA	208	23,167
	10 20 30 40 50 60 MSLVGGFPHH PVMHHDGYSF AAAAAASRCH EEPPYFHGWL ISHPEMSPPD YTMAPSYSPE 70 80 90 100 110 120 YSTGAPGLDH SHYGGVPGAG AVGMGPRTVK RRPTANRKER RRTQSINSAF AELRECIPNV 130 140 150 160 170 180 PADTKLSKIK TLRLATSYIA YLMDILDKDE QNGETEAFKA EFKKTDAKEE RRKKEMNDVL 190 200 KSSGSSNDKK TKGRTGWPQH VWALELKQ

References

[1]	"Conservation of sequence and expression of Xenopus and zebrafish dHAND during cardiac, branchial arch and lateral mesoderm development." Angelo S., Lohr J., Lee K.H., Ticho B.S., Breitbart R.E., Hill S., Yost H.J., Srivastava D. Mech. Dev. 95:231-237(2000) [PubMed: 10906469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"The bHLH transcription factor hand2 plays parallel roles in zebrafish heart and pectoral fin development." Yelon D., Ticho B.S., Halpern M.E., Ruvinsky I., Ho R.K., Silver L.M., Stainier D.Y.R. Development 127:2573-2582(2000) [PubMed: 10821756] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF228334 mRNA. Translation: AAF67130.1.
IPI	IPI00509553.
UniGene	Dr.81423.
3D structure databases
ProteinModelPortal	P57102.
ModBase	Search...
Protein-protein interaction databases
STRING	P57102.
Proteomic databases
PRIDE	P57102.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-000511-1. hand2.
Phylogenomic databases
HOGENOM	HBG717563.
HOVERGEN	HBG051880.
InParanoid	P57102.
OrthoDB	EOG4FTW1T.
Gene expression databases
ArrayExpress	P57102.
Bgee	P57102.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HAND2_DANRE

Accession

Primary (citable) accession number: P57102

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	October 19, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program