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P57094 (AXIN1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Axin-1
Alternative name(s):
Axis inhibition protein 1
Gene names
Name:axin1
Synonyms:axin
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. Ref.4

Subunit structure

Homodimer By similarity. Interacts with dixdc1. Ref.3

Subcellular location

Cytoplasm By similarity.

Domain

The DIX domain mediates interaction with dixdc1.

Post-translational modification

Probably phosphorylated by GSK3B and dephosphorylated by PP2A By similarity.

Sequence similarities

Contains 1 DIX domain.

Contains 1 RGS domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCytoplasm
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway involved in somitogenesis

Inferred from genetic interaction PubMed 19517013. Source: ZFIN

Wnt-activated signaling pathway involved in forebrain neuron fate commitment

Inferred from mutant phenotype PubMed 9010204. Source: ZFIN

activation of JUN kinase activity

Inferred from direct assay Ref.4. Source: ZFIN

axial mesoderm formation

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation

Inferred from mutant phenotype PubMed 11641213. Source: ZFIN

cell death

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to organic cyclic compound

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasmic microtubule organization

Inferred from Biological aspect of Ancestor. Source: RefGenome

determination of left/right symmetry

Inferred from mutant phenotype PubMed 17678853. Source: ZFIN

dorsal/ventral axis specification

Inferred from direct assay Ref.4. Source: MGI

dorsal/ventral pattern formation

Inferred from direct assay Ref.4. Source: MGI

embryonic eye morphogenesis

Inferred from mutant phenotype PubMed 18356958. Source: ZFIN

embryonic skeletal joint morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

eye development

Inferred from mutant phenotype PubMed 16854974. Source: ZFIN

fin regeneration

Inferred from mutant phenotype PubMed 17185322. Source: ZFIN

forebrain anterior/posterior pattern specification

Inferred from mutant phenotype PubMed 11390362. Source: ZFIN

forebrain development

Inferred from mutant phenotype PubMed 9007240. Source: ZFIN

muscle cell development

Inferred from genetic interaction PubMed 19517013. Source: ZFIN

negative regulation of Wnt signaling pathway

Inferred from mutant phenotype PubMed 11390362PubMed 15539488. Source: ZFIN

negative regulation of canonical Wnt signaling pathway

Inferred from mutant phenotype PubMed 11641213. Source: ZFIN

negative regulation of fat cell differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

neural plate anterior/posterior regionalization

Inferred from mutant phenotype PubMed 11390362. Source: ZFIN

olfactory placode formation

Inferred from mutant phenotype PubMed 9007240. Source: ZFIN

optic placode formation

Inferred from mutant phenotype PubMed 9007240. Source: ZFIN

positive regulation of GTPase activity

Inferred from Biological aspect of Ancestor. Source: GOC

positive regulation of JNK cascade

Inferred from mutant phenotype Ref.4. Source: UniProtKB

positive regulation of JUN kinase activity

Inferred from direct assay Ref.4. Source: MGI

regulation of catenin import into nucleus

Inferred from Biological aspect of Ancestor. Source: RefGenome

termination of G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-catenin destruction complex

Inferred from direct assay PubMed 11390362. Source: ZFIN

cell cortex

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasmic membrane-bounded vesicle

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasmic microtubule

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

postsynaptic density

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionGTPase activator activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

armadillo repeat domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

beta-catenin binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein kinase binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Axin-1
PRO_0000220893

Regions

Domain92 – 214123RGS
Domain753 – 83583DIX
Region351 – 43686Interaction with GSK3B By similarity
Region437 – 51276Interaction with beta-catenin By similarity

Experimental info

Sequence conflict1761T → M in BAA92439. Ref.1
Sequence conflict2751P → L in BAA92439. Ref.1
Sequence conflict6581G → S in BAA92439. Ref.1
Sequence conflict7631R → C in BAA92439. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P57094 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: 7ABEE52E1DE2FAB6

FASTA83594,329
        10         20         30         40         50         60 
MSMSVNEKGI CYLPDLGSSF TEDAPRPPVP GEEGDLVSSD GRQYNHSFYS SKSDSLKNEA 

        70         80         90        100        110        120 
SIATPRRPDL DLGYEPEGSA SPTPPYLKWA ESLHSLLDDQ DGIHLFRTFL KQEECADMLD 

       130        140        150        160        170        180 
FWFACSGFRK QEANDGNEKM LKLAKAIYKK YILDNNGIVS RQIKPATKSF IKDCVTKLHI 

       190        200        210        220        230        240 
DPAMFDQAQT EIQTMMEENT YPLFLKSDIY LEYTRTGGES PKLFSDQSSV SGNGKVLPGY 

       250        260        270        280        290        300 
LPTVIEDVEW RCDQEEEQIA ESDPTPSNRL TQKLPLETVP QRVANSKRYQ DNREYRHASW 

       310        320        330        340        350        360 
REPVNPYYVN SGYALAPATS ANDSEQQSMS SDADTLSLTD SSVDGVPPYR YRKPHRREIH 

       370        380        390        400        410        420 
ESAKVNGRVP LPHIPRTNRI PKDIHVEPEK FAAELISRLE GVLREREAQE KLEERLKRVR 

       430        440        450        460        470        480 
LEEEGDDADI STGPSLANHR VPPAVHVQHY GGRYSEMSYN GLQLRDAHEE NPESILDEHV 

       490        500        510        520        530        540 
QRVMKTPGCQ SPGTGRHSPK SRSPDGLPAG KIPGLMMPLS GGQGKHQARQ GPKGEAAHLH 

       550        560        570        580        590        600 
HHKHIHHTHY AAAGKPKEQA EAEAARMHGG FAWNTEQHHY GPKSRNYADG MSVGPNTMDP 

       610        620        630        640        650        660 
MGYSSKGSTL SKRPVRKGED GRNFEMREPL PADDMERNQK ILQWMMEGEK EAGRYKRGPY 

       670        680        690        700        710        720 
GSISGPKKAQ GHEPARPSSV ERLGAVHPWV TAQLRNNVQP SHPFIQDPTM PPNPAPNPLT 

       730        740        750        760        770        780 
QLEEARRRLE EERRKSGTLQ AKQRHKNMKK QPCENITVAY YFRGEPIPYR TSVKGRIVTL 

       790        800        810        820        830 
GQFKELLTKK GSYKYYFKKV SYEFDCGVVF EEVREDDAIL PIFEEKIIGK VEKVD 

« Hide

References

« Hide 'large scale' references
[1]"Cooperative roles of Bozozok/Dharma and Nodal-related proteins in the formation of the dorsal organizer in zebrafish."
Shimizu T., Yamanaka Y., Ryu S.-L., Hashimoto H., Yabe T., Hirata T., Bae Y.-K., Hibi M., Hirano T.
Mech. Dev. 91:293-303(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
[3]"Ccd1, a novel protein with a DIX domain, is a positive regulator in the Wnt signaling during zebrafish neural patterning."
Shiomi K., Uchida H., Keino-Masu K., Masu M.
Curr. Biol. 13:73-77(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DIXDC1.
[4]"A beta-catenin-independent dorsalization pathway activated by Axin/JNK signaling and antagonized by aida."
Rui Y., Xu Z., Xiong B., Cao Y., Lin S., Zhang M., Chan S.-C., Luo W., Han Y., Lu Z., Ye Z., Zhou H.-M., Han J., Meng A., Lin S.-C.
Dev. Cell 13:268-282(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB032262 mRNA. Translation: BAA92439.1.
BC099991 mRNA. Translation: AAH99991.1.
RefSeqNP_571578.2. NM_131503.2.
UniGeneDr.80549.

3D structure databases

ProteinModelPortalP57094.
SMRP57094. Positions 78-223, 752-835.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid78941. 3 interactions.
STRING7955.ENSDARP00000036978.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID57931.
KEGGdre:57931.

Organism-specific databases

CTD8312.
ZFINZDB-GENE-000403-1. axin1.

Phylogenomic databases

eggNOGNOG238205.
HOVERGENHBG004324.
InParanoidQ499B7.
KOK02157.
PhylomeDBP57094.

Family and domain databases

Gene3D1.10.196.10. 2 hits.
InterProIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20891945.
PROP57094.

Entry information

Entry nameAXIN1_DANRE
AccessionPrimary (citable) accession number: P57094
Secondary accession number(s): Q499B7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 22, 2008
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families