Junctional adhesion molecule B precursor

Names and origin

Protein names

Recommended name:
    Junctional adhesion molecule B
      Short name=JAM-B
Alternative name(s):
    Junctional adhesion molecule 2
      Short name=JAM-2
    Vascular endothelial junction-associated molecule
      Short name=VE-JAM
    CD_antigen=CD322

Gene names

Name:	JAM2
Synonyms:	C21orf43, VEJAM
ORF Names:	UNQ219/PRO245

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Subunit structure	Interacts with JAM3. Ref.8
Subcellular location	Cell junction › tight junction By similarity. Cell membrane; Single-pass type I membrane protein By similarity. Note: Localized at tight junctions of both epithelial and endothelial cells By similarity.
Tissue specificity	Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells. Ref.1
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

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Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Tight junction
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell adhesion Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	integral to plasma membrane Ref.1 Non-traceable author statement. Source: UniProtKB tight junction Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Ref.1 Ref.7

Chain

29 – 298

270

Junctional adhesion molecule B

PRO_0000015069

Regions

Topological domain

29 – 238

210

Extracellular Potential

Transmembrane

239 – 259

21

Potential

Topological domain

260 – 298

39

Cytoplasmic Potential

Domain

32 – 127

96

Ig-like V-type

Domain

134 – 238

105

Ig-like C2-type

Amino acid modifications

Glycosylation

98

1

N-linked (GlcNAc...) Ref.10

Glycosylation

187

1

N-linked (GlcNAc...) Potential

Glycosylation

236

1

N-linked (GlcNAc...) Potential

Disulfide bond

50 ↔ 109

Potential

Disulfide bond

155 ↔ 214

Potential

Natural variations

Natural variant

286

1

S → R: dbSNP rs9976382.

VAR_049973

Experimental info

Sequence conflict

270

1

E → G in AAL82538. Ref.3

Sequence conflict

289 – 298

10

DFKHTKSFII → VQWLTPVIPALWKAAAGGSR GQEF in AAQ88727. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P57087-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: CA78E518E22DCAEE
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FASTA

298

33,207

        10         20         30         40         50         60 
MARRSRHRLL LLLLRYLVVA LGYHKAYGFS APKDQQVVTA VEYQEAILAC KTPKKTVSSR 

        70         80         90        100        110        120 
LEWKKLGRSV SFVYYQQTLQ GDFKNRAEMI DFNIRIKNVT RSDAGKYRCE VSAPSEQGQN 

       130        140        150        160        170        180 
LEEDTVTLEV LVAPAVPSCE VPSSALSGTV VELRCQDKEG NPAPEYTWFK DGIRLLENPR 

       190        200        210        220        230        240 
LGSQSTNSSY TMNTKTGTLQ FNTVSKLDTG EYSCEARNSV GYRRCPGKRM QVDDLNISGI 

       250        260        270        280        290 
IAAVVVVALV ISVCGLGVCY AQRKGYFSKE TSFQKSNSSS KATTMSENDF KHTKSFII

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells." Palmeri D., van Zante A., Huang C.-C., Hemmerich S., Rosen S.D. J. Biol. Chem. 275:19139-19145(2000) [PubMed: 10779521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-33, TISSUE SPECIFICITY. Tissue: Vascular endothelial cell.
[2]	"A novel protein with homology to the junctional adhesion molecule: Characterization of leukocyte interactions." Cunningham S.A., Arrate M.P., Rodriguez J.M., Bjercke R.J., Vanderslice P., Morris A.P., Brock T.A. J. Biol. Chem. 275:34750-34756(2000) [PubMed: 10945976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"Annotation of human chromosome 21 for relevance to Down syndrome: gene structure and expression analysis." Gardiner K., Slavov D., Bechtel L., Davisson M. Genomics 79:833-843(2002) [PubMed: 12036298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-43.
[8]	"Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor." Arrate M.P., Rodriguez J.M., Tran T.M., Brock T.A., Cunningham S.A. J. Biol. Chem. 276:45826-45832(2001) [PubMed: 11590146] [Abstract] Cited for: INTERACTION WITH JAM3.
[9]	"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response." Muller W.A. Trends Immunol. 24:327-334(2003) [PubMed: 12810109] [Abstract] Cited for: REVIEW, NOMENCLATURE.
[10]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-98, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF255910 mRNA. Translation: AAF81223.1. AY016009 mRNA. Translation: AAG49022.1. AY077698 mRNA. Translation: AAL82538.1. AY358361 mRNA. Translation: AAQ88727.1. AK312708 mRNA. Translation: BAG35586.1. BC017779 mRNA. Translation: AAH17779.1.
IPI	IPI00299083.
RefSeq	NP_067042.1.
UniGene	Hs.517227
3D structure databases
SMR	P57087. Positions 36-239.
ModBase	Search...
Protein-protein interaction databases
STRING	P57087.
Proteomic databases
PRIDE	P57087.
Genome annotation databases
Ensembl	ENST00000480456; ENSP00000420419; ENSG00000154721; Homo sapiens. [Genome view]
GeneID	58494.
KEGG	hsa:58494.
UCSC	uc002ylp.1. human.
Organism-specific databases
CTD	58494.
GeneCards	GC21P025933.
H-InvDB	HIX0016038.
HGNC	HGNC:14686. JAM2.
MIM	606870. gene.
PharmGKB	PA29992.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09899.
HOVERGEN	P57087.
Enzyme and pathway databases
Pathway_Interaction_DB	amb2_neutrophils_pathway. amb2 Integrin signaling.
Reactome	REACT_13552. Integrin cell surface interactions. REACT_604. Hemostasis. REACT_6900. Signaling in Immune system.
Gene expression databases
ArrayExpress	P57087.
Bgee	P57087.
CleanEx	HS_JAM2.
Genevestigator	P57087.
GermOnline	ENSG00000154721. Homo sapiens.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013106. Ig_V-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	64980.
SOURCE	Search...

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Entry information

Entry name

JAM2_HUMAN

Accession

Primary (citable) accession number: P57087
Secondary accession number(s): B2R6T9, Q6UXG6, Q6YNC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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