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Protein

Junctional adhesion molecule B

Gene

JAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the processes of lymphocyte homing to secondary lymphoid organs.

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule B
Short name:
JAM-B
Alternative name(s):
Junctional adhesion molecule 2
Short name:
JAM-2
Vascular endothelial junction-associated molecule
Short name:
VE-JAM
CD_antigen: CD322
Gene namesi
Name:JAM2
Synonyms:C21orf43, VEJAM
ORF Names:UNQ219/PRO245
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:14686. JAM2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 238210ExtracellularSequence analysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST
Topological domaini260 – 29839CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29992.

Polymorphism and mutation databases

BioMutaiJAM2.
DMDMi10720348.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28282 PublicationsAdd
BLAST
Chaini29 – 298270Junctional adhesion molecule BPRO_0000015069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 109PROSITE-ProRule annotation
Glycosylationi98 – 981N-linked (GlcNAc...)1 Publication
Disulfide bondi155 ↔ 214PROSITE-ProRule annotation
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP57087.
PaxDbiP57087.
PRIDEiP57087.

PTM databases

iPTMnetiP57087.
PhosphoSiteiP57087.
SwissPalmiP57087.

Expressioni

Tissue specificityi

Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.1 Publication

Gene expression databases

BgeeiP57087.
CleanExiHS_JAM2.
ExpressionAtlasiP57087. baseline and differential.
GenevisibleiP57087. HS.

Interactioni

Subunit structurei

Interacts with JAM3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
JAM3Q9BX672EBI-3918416,EBI-4314733

Protein-protein interaction databases

BioGridi121824. 10 interactions.
IntActiP57087. 8 interactions.
MINTiMINT-1344995.
STRINGi9606.ENSP00000420419.

Structurei

3D structure databases

ProteinModelPortaliP57087.
SMRiP57087. Positions 30-221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 12796Ig-like V-typeAdd
BLAST
Domaini134 – 238105Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDXS. Eukaryota.
ENOG41100KX. LUCA.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiP57087.
KOiK06735.
OrthoDBiEOG7MH0Z1.
PhylomeDBiP57087.
TreeFamiTF331459.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57087-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARRSRHRLL LLLLRYLVVA LGYHKAYGFS APKDQQVVTA VEYQEAILAC
60 70 80 90 100
KTPKKTVSSR LEWKKLGRSV SFVYYQQTLQ GDFKNRAEMI DFNIRIKNVT
110 120 130 140 150
RSDAGKYRCE VSAPSEQGQN LEEDTVTLEV LVAPAVPSCE VPSSALSGTV
160 170 180 190 200
VELRCQDKEG NPAPEYTWFK DGIRLLENPR LGSQSTNSSY TMNTKTGTLQ
210 220 230 240 250
FNTVSKLDTG EYSCEARNSV GYRRCPGKRM QVDDLNISGI IAAVVVVALV
260 270 280 290
ISVCGLGVCY AQRKGYFSKE TSFQKSNSSS KATTMSENDF KHTKSFII
Length:298
Mass (Da):33,207
Last modified:December 1, 2000 - v1
Checksum:iCA78E518E22DCAEE
GO
Isoform 2 (identifier: P57087-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-79: Missing.

Show »
Length:262
Mass (Da):29,037
Checksum:i9892E90CBCB97C74
GO
Isoform 3 (identifier: P57087-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     289-298: DFKHTKSFII → VQWLTPVIPALWKAAAGGSRGQEF

Note: No experimental confirmation available.
Show »
Length:312
Mass (Da):34,554
Checksum:i7FAE85F8B54D7B0A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti270 – 2701E → G in AAL82538 (PubMed:12036298).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti286 – 2861S → R.
Corresponds to variant rs9976382 [ dbSNP | Ensembl ].
VAR_049973

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei44 – 7936Missing in isoform 2. 1 PublicationVSP_045153Add
BLAST
Alternative sequencei289 – 29810DFKHTKSFII → VQWLTPVIPALWKAAAGGSR GQEF in isoform 3. 1 PublicationVSP_047352

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255910 mRNA. Translation: AAF81223.1.
AY016009 mRNA. Translation: AAG49022.1.
AY077698 mRNA. Translation: AAL82538.1.
AY358361 mRNA. Translation: AAQ88727.1.
AK294769 mRNA. Translation: BAG57900.1.
AK312708 mRNA. Translation: BAG35586.1.
AP000223 Genomic DNA. No translation available.
AP000224 Genomic DNA. No translation available.
AP000225 Genomic DNA. No translation available.
AP000226 Genomic DNA. No translation available.
BC017779 mRNA. Translation: AAH17779.1.
CCDSiCCDS42911.1. [P57087-1]
CCDS58787.1. [P57087-3]
CCDS58788.1. [P57087-2]
RefSeqiNP_001257336.1. NM_001270407.1. [P57087-2]
NP_001257337.1. NM_001270408.1. [P57087-3]
NP_067042.1. NM_021219.3. [P57087-1]
UniGeneiHs.517227.

Genome annotation databases

EnsembliENST00000312957; ENSP00000318416; ENSG00000154721. [P57087-2]
ENST00000400532; ENSP00000383376; ENSG00000154721. [P57087-3]
ENST00000480456; ENSP00000420419; ENSG00000154721. [P57087-1]
GeneIDi58494.
KEGGihsa:58494.
UCSCiuc002ylp.3. human. [P57087-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255910 mRNA. Translation: AAF81223.1.
AY016009 mRNA. Translation: AAG49022.1.
AY077698 mRNA. Translation: AAL82538.1.
AY358361 mRNA. Translation: AAQ88727.1.
AK294769 mRNA. Translation: BAG57900.1.
AK312708 mRNA. Translation: BAG35586.1.
AP000223 Genomic DNA. No translation available.
AP000224 Genomic DNA. No translation available.
AP000225 Genomic DNA. No translation available.
AP000226 Genomic DNA. No translation available.
BC017779 mRNA. Translation: AAH17779.1.
CCDSiCCDS42911.1. [P57087-1]
CCDS58787.1. [P57087-3]
CCDS58788.1. [P57087-2]
RefSeqiNP_001257336.1. NM_001270407.1. [P57087-2]
NP_001257337.1. NM_001270408.1. [P57087-3]
NP_067042.1. NM_021219.3. [P57087-1]
UniGeneiHs.517227.

3D structure databases

ProteinModelPortaliP57087.
SMRiP57087. Positions 30-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121824. 10 interactions.
IntActiP57087. 8 interactions.
MINTiMINT-1344995.
STRINGi9606.ENSP00000420419.

PTM databases

iPTMnetiP57087.
PhosphoSiteiP57087.
SwissPalmiP57087.

Polymorphism and mutation databases

BioMutaiJAM2.
DMDMi10720348.

Proteomic databases

MaxQBiP57087.
PaxDbiP57087.
PRIDEiP57087.

Protocols and materials databases

DNASUi58494.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312957; ENSP00000318416; ENSG00000154721. [P57087-2]
ENST00000400532; ENSP00000383376; ENSG00000154721. [P57087-3]
ENST00000480456; ENSP00000420419; ENSG00000154721. [P57087-1]
GeneIDi58494.
KEGGihsa:58494.
UCSCiuc002ylp.3. human. [P57087-1]

Organism-specific databases

CTDi58494.
GeneCardsiJAM2.
HGNCiHGNC:14686. JAM2.
MIMi606870. gene.
neXtProtiNX_P57087.
PharmGKBiPA29992.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IDXS. Eukaryota.
ENOG41100KX. LUCA.
GeneTreeiENSGT00730000110678.
HOGENOMiHOG000247041.
HOVERGENiHBG000518.
InParanoidiP57087.
KOiK06735.
OrthoDBiEOG7MH0Z1.
PhylomeDBiP57087.
TreeFamiTF331459.

Enzyme and pathway databases

ReactomeiR-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-216083. Integrin cell surface interactions.

Miscellaneous databases

ChiTaRSiJAM2. human.
GeneWikiiJAM2.
GenomeRNAii58494.
PROiP57087.
SOURCEiSearch...

Gene expression databases

BgeeiP57087.
CleanExiHS_JAM2.
ExpressionAtlasiP57087. baseline and differential.
GenevisibleiP57087. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells."
    Palmeri D., van Zante A., Huang C.-C., Hemmerich S., Rosen S.D.
    J. Biol. Chem. 275:19139-19145(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 29-33, TISSUE SPECIFICITY.
    Tissue: Vascular endothelial cell.
  2. "A novel protein with homology to the junctional adhesion molecule: Characterization of leukocyte interactions."
    Cunningham S.A., Arrate M.P., Rodriguez J.M., Bjercke R.J., Vanderslice P., Morris A.P., Brock T.A.
    J. Biol. Chem. 275:34750-34756(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "Annotation of human chromosome 21 for relevance to Down syndrome: gene structure and expression analysis."
    Gardiner K., Slavov D., Bechtel L., Davisson M.
    Genomics 79:833-843(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  6. "The DNA sequence of human chromosome 21."
    Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
    , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
    Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  8. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-43.
  9. "Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor."
    Arrate M.P., Rodriguez J.M., Tran T.M., Brock T.A., Cunningham S.A.
    J. Biol. Chem. 276:45826-45832(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH JAM3.
  10. "Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response."
    Muller W.A.
    Trends Immunol. 24:327-334(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, NOMENCLATURE.
  11. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-98.
    Tissue: Plasma.

Entry informationi

Entry nameiJAM2_HUMAN
AccessioniPrimary (citable) accession number: P57087
Secondary accession number(s): B2R6T9
, B4DGT9, Q6UXG6, Q6YNC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 8, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.