Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P57080

- UBP25_MOUSE

UniProt

P57080 - UBP25_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 25

Gene

Usp25

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (13 Apr 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains By similarity.By similarity
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei178 – 1781NucleophilePROSITE-ProRule annotation
    Active sitei608 – 6081Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: MGI

    GO - Biological processi

    1. protein K48-linked deubiquitination Source: Ensembl
    2. protein K63-linked deubiquitination Source: Ensembl
    3. ubiquitin-dependent protein catabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.041.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 25 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 25
    Ubiquitin thioesterase 25
    Ubiquitin-specific-processing protease 25
    Short name:
    mUSP25
    Gene namesi
    Name:Usp25
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1353655. Usp25.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: The longer muscle-specific isoform (USP25m) Some transient punctuate nuclear location in myotubes during myocyte development.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10551055Ubiquitin carboxyl-terminal hydrolase 25PRO_0000080654Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki99 – 99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
    Cross-linki99 – 99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Modified residuei740 – 7401PhosphotyrosineBy similarity

    Post-translational modificationi

    Acetylated.By similarity
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue By similarity.By similarity
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions By similarity.By similarity
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP57080.
    PaxDbiP57080.
    PRIDEiP57080.

    PTM databases

    PhosphoSiteiP57080.

    Expressioni

    Tissue specificityi

    Highly expressed in testis especially in primary and secondary spematocytes and in immature spermatids. Also found in brain, skeletal muscle, liver and heart.1 Publication

    Developmental stagei

    At E13.5 and E16.5, expression in the brain correlates with the proliferate ventricular zone and post-mitotic neurons of the intermediate zone, particularly in the forebrain. More marked expression at E16.5 in the telencephalic septum and in the pallium. In myocytes, expressed throughout differentiation of myotubes.2 Publications

    Gene expression databases

    ArrayExpressiP57080.
    BgeeiP57080.
    CleanExiMM_USP25.
    GenevestigatoriP57080.

    Interactioni

    Subunit structurei

    Homodimer or oligomer By similarity. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels By similarity.By similarity

    Protein-protein interaction databases

    IntActiP57080. 2 interactions.

    Structurei

    Secondary structure

    1
    1055
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 124
    Helixi17 – 2711
    Helixi33 – 4311
    Helixi47 – 559

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VDLNMR-A1-67[»]
    ProteinModelPortaliP57080.
    SMRiP57080. Positions 1-138, 169-407, 592-677.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP57080.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 5744UBA-likeAdd
    BLAST
    Repeati97 – 11620UIM 1Add
    BLAST
    Repeati123 – 14018UIM 2Add
    BLAST
    Domaini169 – 658490USPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni77 – 10226SUMO interaction domain (SIM)Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi89 – 957Required for SUMO paralog-specific binding

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 UBA-like domain.Curated
    Contains 2 UIM (ubiquitin-interacting motif) repeats.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG5077.
    GeneTreeiENSGT00390000016082.
    HOGENOMiHOG000007956.
    HOVERGENiHBG056030.
    InParanoidiP57080.
    KOiK11849.
    OMAiSRSVIHK.
    OrthoDBiEOG761BSZ.
    PhylomeDBiP57080.
    TreeFamiTF329035.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR003903. Ubiquitin-int_motif.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02809. UIM. 2 hits.
    [Graphical view]
    SMARTiSM00726. UIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    PROSITEiPS50330. UIM. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A longer muscle-specific isoform, USP25m, also exists.

    Isoform 1 (identifier: P57080-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDAQILQQAL KDSNGNLELA     50
    VAFLTAKNAK TPPQEETGYY QTALPGNDRY ISVGSQADAN VIDLTGDDKD 100
    DLQRAIALSL AESNRAFRET GITDEEQAIS RVLEASIAEN KACLKRTPIE 150
    VWRDSRNPYD RKRQEKAPVG LKNVGNTCWF SAVIQSLFNL LEFRRLVLNY 200
    KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV DPSRAVEILK 250
    DAFKSNDSQQ QDVSEFTHKL LDWLEDAFQM KAEEETDEEK PKNPMVELFY 300
    GRFLAMGVLE GKKFENTEMF GQYPLQVNGF KDLHECLEAA MIEGEIESLH 350
    SDNSGKSGQE HWFTELPPVL TFELSRFEFN QALGRPEKIH NKLEFPQVLY 400
    LDRYMHRNRE ITRIKREEIK RLKDYLTVLQ QRLERYLSYG SGPKRFPLVD 450
    VLQYALEFAS SKPVCTSPVD DIDASSSASG PLPSQSLPST TEQQGPCASD 500
    LPGSSSPASG AALPLRSVIH KPFTQSRIPP DLPMHPAPRH ITEEELCVLE 550
    SCLHRWRTEI ENDTRDLQES ISRIHRTIEL MYSDKSMIQV PYRLHAVLVH 600
    EGQANAGHYW AYIFDHRESR WMKYNDIAVT KSSWEELVRD SFGGYRNASA 650
    YCLMYIDDKA QFLIQEEFNK ETGQALVGME TLPPDLRDFV EEDNQRFEKE 700
    LEEWDTQLAQ RSLQEKLLAA PKLREAEASA TTAQAGGADY LEQPSRSDLS 750
    KHWKEETLRV IAKASHDLED KGPETVLQSA IKLEYSRLVK LAQEDTPPET 800
    DYRLHHVLVY FIQNQAPKKI IEKTLLEQFG DRNLSFDERC HNIMKVAQAK 850
    LEMIKPEEVN LEEYEEWHAD YKKFRETTMY LITGLENFQR ESYIDSLLFL 900
    LCAYQNNKEL LSKGPYRGHD GELISHYRRE CLLKLNEQAA ELFESGEDGD 950
    VNNGLIIMNE FIVPFLPLLL VDDMEEKDIL AVEDMRNRWC SYLGQEMEAN 1000
    LQEKLTDFLP KLLDCSTEIK GFHEPPKLPS YSAHELCERF ARIMLSLSRT 1050
    PADGR 1055
    Length:1,055
    Mass (Da):121,420
    Last modified:April 13, 2004 - v2
    Checksum:i103E34EC3FA8A72B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti394 – 3941E → K in AAF32264. (PubMed:10644437)Curated
    Sequence conflicti496 – 4961P → L in AAF32264. (PubMed:10644437)Curated
    Sequence conflicti683 – 6831P → L in AAF32264. (PubMed:10644437)Curated
    Sequence conflicti841 – 8411H → L in AAF32264. (PubMed:10644437)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF170563 mRNA. Translation: AAF32264.1.
    BC048171 mRNA. Translation: AAH48171.1.
    BC063059 mRNA. Translation: AAH63059.1.
    CCDSiCCDS28275.1. [P57080-1]
    RefSeqiNP_038946.2. NM_013918.2. [P57080-1]
    UniGeneiMm.40986.

    Genome annotation databases

    EnsembliENSMUST00000023580; ENSMUSP00000023580; ENSMUSG00000022867. [P57080-1]
    GeneIDi30940.
    KEGGimmu:30940.
    UCSCiuc007zsb.1. mouse. [P57080-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF170563 mRNA. Translation: AAF32264.1 .
    BC048171 mRNA. Translation: AAH48171.1 .
    BC063059 mRNA. Translation: AAH63059.1 .
    CCDSi CCDS28275.1. [P57080-1 ]
    RefSeqi NP_038946.2. NM_013918.2. [P57080-1 ]
    UniGenei Mm.40986.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VDL NMR - A 1-67 [» ]
    ProteinModelPortali P57080.
    SMRi P57080. Positions 1-138, 169-407, 592-677.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P57080. 2 interactions.

    Protein family/group databases

    MEROPSi C19.041.

    PTM databases

    PhosphoSitei P57080.

    Proteomic databases

    MaxQBi P57080.
    PaxDbi P57080.
    PRIDEi P57080.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023580 ; ENSMUSP00000023580 ; ENSMUSG00000022867 . [P57080-1 ]
    GeneIDi 30940.
    KEGGi mmu:30940.
    UCSCi uc007zsb.1. mouse. [P57080-1 ]

    Organism-specific databases

    CTDi 29761.
    MGIi MGI:1353655. Usp25.

    Phylogenomic databases

    eggNOGi COG5077.
    GeneTreei ENSGT00390000016082.
    HOGENOMi HOG000007956.
    HOVERGENi HBG056030.
    InParanoidi P57080.
    KOi K11849.
    OMAi SRSVIHK.
    OrthoDBi EOG761BSZ.
    PhylomeDBi P57080.
    TreeFami TF329035.

    Miscellaneous databases

    ChiTaRSi USP25. mouse.
    EvolutionaryTracei P57080.
    NextBioi 307378.
    PROi P57080.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P57080.
    Bgeei P57080.
    CleanExi MM_USP25.
    Genevestigatori P57080.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR003903. Ubiquitin-int_motif.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02809. UIM. 2 hits.
    [Graphical view ]
    SMARTi SM00726. UIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    PROSITEi PS50330. UIM. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2."
      Valero R., Marfany G., Gonzalez-Angulo O., Gonzalez-Gonzalez G., Puelles L., Gonzalez-Duarte R.
      Genomics 62:395-405(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Solution structure of RSGI RUH-013, a UBA domain in mouse."
      RIKEN structural genomics initiative (RSGI)
      Submitted (SEP-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 1-67.
    4. "The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins."
      Bosch-Comas A., Lindsten K., Gonzalez-Duarte R., Masucci M.G., Marfany G.
      Cell. Mol. Life Sci. 63:723-734(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE, POSSIBLE FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiUBP25_MOUSE
    AccessioniPrimary (citable) accession number: P57080
    Secondary accession number(s): Q80ZT9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3