Reviewed, UniProtKB/Swiss-Prot P57058 (HUNK_HUMAN)

Names and origin

Protein names

Recommended name:
    Hormonally up-regulated neu tumor-associated kinase
    EC=2.7.11.1
Alternative name(s):
    Serine/threonine-protein kinase MAK-V
    B19

Gene names

Name:	HUNK
Synonyms:	MAKV

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Ubl conjugation
Gene Ontology (GO)
Biological process	multicellular organismal development Traceable author statement. Source: ProtInc protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Hormonally up-regulated neu tumor-associated kinase		PRO_0000086004
Regions
	Domain	62 – 320	259	Protein kinase
	Nucleotide binding	68 – 76	9	ATP By similarity
Sites
	Active site	186	1	Proton acceptor By similarity
	Binding site	91	1	ATP By similarity
Amino acid modifications
	Cross-link	483		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Natural variations
	Natural variant	157	1	R → W		VAR_040561
	Natural variant	591	1	R → C		VAR_040562
	Natural variant	625	1	E → K		VAR_040563
	Natural variant	648	1	M → T		VAR_040564

Sequences

	Sequence		Length	Mass (Da)
	P57058-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 87FC85C67D838AA7	FASTA	714	79,686
	10 20 30 40 50 60 MPAAAGDGLL GEPAAPGGGG GAEDAARPAA ACEGSFLPAW VSGVPRERLR DFQHHKRVGN 70 80 90 100 110 120 YLIGSRKLGE GSFAKVREGL HVLTGEKVAI KVIDKKRAKK DTYVTKNLRR EGQIQQMIRH 130 140 150 160 170 180 PNITQLLDIL ETENSYYLVM ELCPGGNLMH KIYEKKRLEE SEARRYIRQL ISAVEHLHRA 190 200 210 220 230 240 GVVHRDLKIE NLLLDEDNNI KLIDFGLSNC AGILGYSDPF STQCGSPAYA APELLARKKY 250 260 270 280 290 300 GPKIDVWSIG VNMYAMLTGT LPFTVEPFSL RALYQKMVDK EMNPLPTQLS TGAISFLRSL 310 320 330 340 350 360 LEPDPVKRPN IQQALANRWL NENYTGKVPC NVTYPNRISL EDLSPSVVLH MTEKLGYKNS 370 380 390 400 410 420 DVINTVLSNR ACHILAIYFL LNKKLERYLS GKSDIQDSLC YKTRLYQIEK YRAPKESYEA 430 440 450 460 470 480 SLDTWTRDLE FHAVQDKKPK EQEKRGDFLH RPFSKKLDKN LPSHKQPSGS LMTQIQNTKA 490 500 510 520 530 540 LLKDRKASKS SFPDKDSFGC RNIFRKTSDS NCVASSSMEF IPVPPPRTPR IVKKPEPHQP 550 560 570 580 590 600 GPGSTGIPHK EDPLMLDMVR SFESVDRDDH VEVLSPSHHY RILNSPVSLA RRNSSERTLS 610 620 630 640 650 660 PGLPSGSMSP LHTPLHPTLV SFAHEDKNSP PKEEGLCCPP PVPSNGPMQP LGSPNCVKSR 670 680 690 700 710 GRFPMMGIGQ MLRKRHQSLQ PSADRPLEAS LPPLQPLAPV NLAFDMADGV KTQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative serine/threonine protein kinase MAK-V on human chromosome 21q22.1." Scott H.S., Antonarakis S.E., Papasavvas M.P., Michaud J. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D. J. Proteome Res. 6:298-305(2007) [PubMed: 17203973] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-483, MASS SPECTROMETRY. Tissue: Lung adenocarcinoma.
[3]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-157; CYS-591; LYS-625 AND THR-648.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ271722 mRNA. Translation: CAB71146.1.
RefSeq	NP_055401.1.
UniGene	Hs.109437
3D structure databases
HSSP	HSSP built from PDB template 1A06 based on UniProtKB Q63450.
ModBase	Search...
PTM databases
PhosphoSite	P57058.
Genome annotation databases
Ensembl	ENSG00000142149. Homo sapiens. [Contig view]
GeneID	30811.
KEGG	hsa:30811.
Organism-specific databases
HGNC	HGNC:13326. HUNK.
MIM	606532. gene.
PharmGKB	PA29563.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P57058.
HOVERGEN	P57058.
Gene expression databases
ArrayExpress	P57058.
CleanEx	HS_HUNK.
GermOnline	ENSG00000142149. Homo sapiens.
Family and domain databases
InterPro	IPR013334. Hormone-upReg_Neu-assoc_kinase. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22982:SF36. Hormone-upReg_Neu-assoc_kinase. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P57058.
NextBio	52856.
SOURCE	Search...

Entry information

Entry name

HUNK_HUMAN

Accession

Primary (citable) accession number: P57058

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)