P57058 (HUNK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hormonally up-regulated neu tumor-associated kinase
EC=2.7.11.1

Alternative name(s):
B19
Serine/threonine-protein kinase MAK-V

Gene names

Name:	HUNK
Synonyms:	MAKV

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Traceable author statement. Source: ProtInc signal transduction Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Hormonally up-regulated neu tumor-associated kinase		PRO_0000086004
Regions
	Domain	62 – 320	259	Protein kinase
	Nucleotide binding	68 – 76	9	ATP By similarity
Sites
	Active site	186	1	Proton acceptor By similarity
	Binding site	91	1	ATP By similarity
Amino acid modifications
	Cross-link	483		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.2
Natural variations
	Natural variant	157	1	R → W. Ref.3 Corresponds to variant rs35133981 [ dbSNP | Ensembl ].		VAR_040561
	Natural variant	591	1	R → C. Ref.3 Corresponds to variant rs10775648 [ dbSNP | Ensembl ].		VAR_040562
	Natural variant	625	1	E → K. Ref.3 Corresponds to variant rs56021554 [ dbSNP | Ensembl ].		VAR_040563
	Natural variant	648	1	M → T. Ref.3 Corresponds to variant rs56240027 [ dbSNP | Ensembl ].		VAR_040564

Sequences

	Sequence		Length	Mass (Da)
	P57058 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 87FC85C67D838AA7	FASTA	714	79,686
	10 20 30 40 50 60 MPAAAGDGLL GEPAAPGGGG GAEDAARPAA ACEGSFLPAW VSGVPRERLR DFQHHKRVGN 70 80 90 100 110 120 YLIGSRKLGE GSFAKVREGL HVLTGEKVAI KVIDKKRAKK DTYVTKNLRR EGQIQQMIRH 130 140 150 160 170 180 PNITQLLDIL ETENSYYLVM ELCPGGNLMH KIYEKKRLEE SEARRYIRQL ISAVEHLHRA 190 200 210 220 230 240 GVVHRDLKIE NLLLDEDNNI KLIDFGLSNC AGILGYSDPF STQCGSPAYA APELLARKKY 250 260 270 280 290 300 GPKIDVWSIG VNMYAMLTGT LPFTVEPFSL RALYQKMVDK EMNPLPTQLS TGAISFLRSL 310 320 330 340 350 360 LEPDPVKRPN IQQALANRWL NENYTGKVPC NVTYPNRISL EDLSPSVVLH MTEKLGYKNS 370 380 390 400 410 420 DVINTVLSNR ACHILAIYFL LNKKLERYLS GKSDIQDSLC YKTRLYQIEK YRAPKESYEA 430 440 450 460 470 480 SLDTWTRDLE FHAVQDKKPK EQEKRGDFLH RPFSKKLDKN LPSHKQPSGS LMTQIQNTKA 490 500 510 520 530 540 LLKDRKASKS SFPDKDSFGC RNIFRKTSDS NCVASSSMEF IPVPPPRTPR IVKKPEPHQP 550 560 570 580 590 600 GPGSTGIPHK EDPLMLDMVR SFESVDRDDH VEVLSPSHHY RILNSPVSLA RRNSSERTLS 610 620 630 640 650 660 PGLPSGSMSP LHTPLHPTLV SFAHEDKNSP PKEEGLCCPP PVPSNGPMQP LGSPNCVKSR 670 680 690 700 710 GRFPMMGIGQ MLRKRHQSLQ PSADRPLEAS LPPLQPLAPV NLAFDMADGV KTQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative serine/threonine protein kinase MAK-V on human chromosome 21q22.1." Scott H.S., Antonarakis S.E., Papasavvas M.P., Michaud J. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D. J. Proteome Res. 6:298-305(2007) [PubMed: 17203973] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-483, MASS SPECTROMETRY. Tissue: Lung adenocarcinoma.
[3]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-157; CYS-591; LYS-625 AND THR-648.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ271722 mRNA. Translation: CAB71146.1.
IPI	IPI00219553.
RefSeq	NP_055401.1. NM_014586.1.
UniGene	Hs.109437.
3D structure databases
ProteinModelPortal	P57058.
SMR	P57058. Positions 54-383.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-58064N.
IntAct	P57058. 1 interaction.
STRING	P57058.
PTM databases
PhosphoSite	P57058.
Polymorphism databases
DMDM	9973393.
Proteomic databases
PRIDE	P57058.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000270112; ENSP00000270112; ENSG00000142149.
GeneID	30811.
KEGG	hsa:30811.
UCSC	uc002yph.1. human.
Organism-specific databases
CTD	30811.
GeneCards	GC21P033245.
H-InvDB	HIX0040862.
HGNC	HGNC:13326. HUNK.
MIM	606532. gene.
neXtProt	NX_P57058.
PharmGKB	PA29563.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17038.
GeneTree	ENSGT00600000084026.
HOGENOM	HBG715854.
HOVERGEN	HBG006086.
InParanoid	P57058.
OMA	NVTYPNR.
OrthoDB	EOG44F68J.
PhylomeDB	P57058.
Gene expression databases
ArrayExpress	P57058.
Bgee	P57058.
CleanEx	HS_HUNK.
Genevestigator	P57058.
GermOnline	ENSG00000142149. Homo sapiens.
Family and domain databases
InterPro	IPR013334. Hormone-upReg_Neu-assoc_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08797.
PANTHER	PTHR24343:SF36. PTHR24343:SF36. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	52856.
SOURCE	Search...

Entry information

Entry name

HUNK_HUMAN

Accession

Primary (citable) accession number: P57058

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.