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Reviewed, UniProtKB/Swiss-Prot P57001 (CYSG_NEIMA)

Last modified November 25, 2008. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Siroheme synthase
Including the following 3 domains:
    1- Recommended name:
            Uroporphyrinogen-III C-methyltransferase
                Short name=Urogen III methylase
              EC=2.1.1.107
        Alternative name(s):
            SUMT
            Uroporphyrinogen III methylase
              Short name=UROM
    2- Recommended name:
            Precorrin-2 dehydrogenase
              EC=1.3.1.76
    3- Recommended name:
            Sirohydrochlorin ferrochelatase
              EC=4.99.1.4
Gene names
Name: cysG
Ordered Locus Names: NMA1367
OrganismNeisseria meningitidis serogroup A [Complete proteome] [HAMAP]
Taxonomic identifier65699 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Multifunctional enzyme that catalyze the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity.

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Precorrin-2 + NAD(+) = sirohydrochlorin + NADH.

Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+).

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

Belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Siroheme synthase
PRO_0000150381

Regions

Region219 – 459241Uroporphyrinogen-III C-methyltransferase

Sequences

Sequence LengthMass (Da)Tools
P57001-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: F70D6A5F50FF23CF

FASTA48652,484
        10         20         30         40         50         60 
MNYFPIFANL AGRPVLVVGG GAVAARKISL LLKAGAEVRV AAKHLNAELS ALAAENKILW 

        70         80         90        100        110        120 
LAEEFRAEHI RTVFLIIAAS SDQALNRRVF HLAESCQKPV NVVDDRDHCS FIFPSVIDRN 

       130        140        150        160        170        180 
PVQIAVSSSG SAPVLARLLR ERLEALLPPS LGDMAEISGR WRDAVKGKLK SVTERRRFWE 

       190        200        210        220        230        240 
KQFNGRFAAL VKNRQNTLAE RELAGQLEQS RQNDQGGSVS LVGAGPGDAG LLTLKGLQEI 

       250        260        270        280        290        300 
QQADVVLYDA LVSDGILSLV RRDAERIFVG KRARGGRTPQ EDTNALMVRL AREGRRVVRL 

       310        320        330        340        350        360 
KGGDPFVFGR GGEELETLAR HQIPFSVVPG ITAAVGATAY AGIPLTHRDY AQSAVFVTGH 

       370        380        390        400        410        420 
RKADAPDIEW QTLARSRQTL VIYMGALKAA LIAERLQQHG RSPDTPAAVI SQGTLPAQKT 

       430        440        450        460        470        480 
ATGTLANLAE LAETAPNPAL IVIGEVVGLH EKLAWFGENA KKESNPAEHA YFALDGLGTG 


QEQQAA 

« Hide

Cross-references

Sequence databases

AL157959 Genomic DNA. Translation: CAM08540.1.
PIRA81906.

3D structure databases

HSSPHSSP built from PDB template 1CBF based on UniProtKB O87696.
ModBaseSearch...

Genome annotation databases

GenomeReviewsGene locus NMA1367 in contig AL157959_GR.
KEGGnma:NMA1367.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP57001.

Enzyme and pathway databases

BioCycNMEN122587:NMA1367-MON.

Family and domain databases

HAMAPMF_01646.
[Tree]
InterProIPR000878. 4pyrrol_Mease.
IPR006366. CobA_cysG_C.
IPR006367. CysG_synth_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamPF00590. TP_methylase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP57001.

Entry information

Entry nameCYSG_NEIMA
AccessionPrimary (citable) accession number: P57001
Secondary accession number(s): A1IRZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents