P57000 (SYQ_NEIMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	NMA1748

Organism

Neisseria meningitidis serogroup A / serotype 4A (strain Z2491) [Complete proteome] [HAMAP]

Taxonomic identifier

122587 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup A

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence CAM08876.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_0000195838
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	271 – 275	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57000 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 9F7A0747E427EB97	FASTA	562	64,770
	10 20 30 40 50 60 MLNKDQFADN HFIRTIIEDD LKSGKHEAVQ TRFPPEPNGY LHIGHAKSIC LNFGLAYIYD 70 80 90 100 110 120 GLCNLRFDDT NPEKENDEYV NAIKEDVEWL GFHWAGEPRF ASNYFDQLYD YAVGLIKDGK 130 140 150 160 170 180 AYVDDLTPEE MREYRGTLTE AGKNSPYRDR SVEENLDLFT RMKNGEFPDG SKTLRLKIDM 190 200 210 220 230 240 ASGNINMRDP VIYRIRRAHH HNTGDKWCIY PMYDYTHCIS DAIEGITHSL CTLEFEAHRP 250 260 270 280 290 300 LYDWVLDNIH APHATRPRQY EFSRLELLYT ITSKRKLNQL VVEKHVSGWD DPRMPTISGM 310 320 330 340 350 360 RRRGYTPEGL RLFAKRAGIS KSENIVDMSV LEGAIREELE NSAPRLMAVL NPLKVTLTNF 370 380 390 400 410 420 EAGRTQSRRA AFHPNHEEMG EREVPISQTI YIEADDFAEN PPKGFKRLIP GGEVRLRHGY 430 440 450 460 470 480 VIKCDEVVKD EAGNVVELKC SIDHDTLGKN PEGRKVKGVI HWVSAEHAAE IKVRLYDRLF 490 500 510 520 530 540 TIERPDAVRG EDGEYLPFTD FLNPESVKEI TAYAEPAAKD LPAESRWQFE RIGYFVTDRK 550 560 DHGKDTPVFN RTVTLKDSWQ PK

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL157959 Genomic DNA. Translation: CAM08876.1. Different initiation.
RefSeq	YP_002343040.1. NC_003116.1.
3D structure databases
ProteinModelPortal	P57000.
SMR	P57000. Positions 11-558.
ModBase	Search...
Protein-protein interaction databases
STRING	122587.NMA1748.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM08876; CAM08876; NMA1748.
GeneID	907673.
KEGG	nma:NMA1748.
PATRIC	20364786. VBINeiMen132687_2096.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	NMEN122587:GI3Q-1547-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_NEIMA

Accession

Primary (citable) accession number: P57000
Secondary accession number(s): A1ISW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program