P56985 (GALE_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 4-epimerase
EC=5.1.3.2

Alternative name(s):
Galactowaldenase
UDP-galactose 4-epimerase

Gene names

Name:	galE
Ordered Locus Names:	NMB0064

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an essential role in the incorporation of galactose into meningococcal lipopolysaccharide surface molecules, which are important for pathogenesis.
Catalytic activity	UDP-glucose = UDP-galactose.
Cofactor	NAD.
Pathway	Carbohydrate metabolism; galactose metabolism.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the sugar epimerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	NAD
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-glucose 4-epimerase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	UDP-glucose 4-epimerase		PRO_0000183213
Regions
	Nucleotide binding	3 – 34	32	NAD By similarity
Sites
	Active site	150	1	Proton acceptor By similarity
	Binding site	125	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56985 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 7D702C44F07DA99B	FASTA	339	37,062
	10 20 30 40 50 60 MKKILVTGGT GFIGSHTVVS LLKSGHQVVI LDNLCNSSIN ILPRLKTITG QEIPFYQGDI 70 80 90 100 110 120 RDREILRRIF AENRIDSVIH FAGLKAVGES VAEPMKYYDN NVSGSLVLAE EMARAGVFSI 130 140 150 160 170 180 VFSSSATVYG DPGKVPYTED MPPGDTTSPY GASKSMVERI LTDIQKADPR WSMILLRYFN 190 200 210 220 230 240 PIGAHESGLI GEQPNGIPNN LLPYICQVAA GKLPQLAVFG DDYPTPDGTG MRDYIHVMDL 250 260 270 280 290 300 AEGHVAAMQA KSNVAGTHLL NLGSGRASSV LEIIRAFEAA SGLTIPYEVK PRRAGDLACF 310 320 330 YADPSYTKAQ IGWQTQRDLT QMMEDSWRWV SNNPNGYDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and molecular analysis of the galE gene of Neisseria meningitidis and its role in lipopolysaccharide biosynthesis." Jennings M.P., van der Ley P., Wilks K.E., Maskell D.J., Poolman J.T., Moxon E.R. Mol. Microbiol. 10:361-369(1993) [PubMed: 7934827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MC58 / Serogroup B.
[2]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20495 Genomic DNA. Translation: AAA65535.1. AE002098 Genomic DNA. Translation: AAF40532.1.
PIR	S39638.
RefSeq	NP_273128.1. NC_003112.2.
3D structure databases
ProteinModelPortal	P56985.
SMR	P56985. Positions 1-339.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000010468; EBNEIP00000010088; EBNEIG00000010468.
GeneID	902171.
GenomeReviews	Gene locus NMB0064 in contig AE002098_GR.
KEGG	nme:NMB0064.
NMPDR	fig|122586.1.peg.62.
PATRIC	20355133. VBINeiMen85645_0100.
TIGR	NMB0064.
Phylogenomic databases
GeneTree	EBGT00050000020552.
HOGENOM	HBG755066.
OMA	ERIMADY.
ProtClustDB	CLSK877381.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0064-MONOMER.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR005886. GalE. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01784.
PANTHER	PTHR10366:SF39. GalE. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01179. GalE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALE_NEIMB

Accession

Primary (citable) accession number: P56985
Secondary accession number(s): Q59617, Q59624

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program