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Protein

UvrABC system protein B

Gene

uvrB

Organism
Bacillus caldotenax
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).By similarity

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein B
Short name:
Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene namesi
Name:uvrB
OrganismiBacillus caldotenax
Taxonomic identifieri1395 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi98 – 113QPEAY…YIEKD → G: Inactive in incision, unable to form a stable complex with DNA. 1 PublicationAdd BLAST16

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001383792 – 658UvrABC system protein BAdd BLAST657

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.

Structurei

Secondary structure

1658
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 30Combined sources13
Beta strandi34 – 40Combined sources7
Helixi46 – 57Combined sources12
Beta strandi61 – 64Combined sources4
Helixi68 – 81Combined sources14
Beta strandi85 – 90Combined sources6
Helixi93 – 95Combined sources3
Beta strandi96 – 98Combined sources3
Beta strandi100 – 103Combined sources4
Turni104 – 107Combined sources4
Beta strandi108 – 110Combined sources3
Beta strandi113 – 116Combined sources4
Helixi119 – 130Combined sources12
Turni131 – 133Combined sources3
Beta strandi137 – 141Combined sources5
Helixi143 – 146Combined sources4
Beta strandi147 – 149Combined sources3
Helixi153 – 156Combined sources4
Beta strandi160 – 165Combined sources6
Turni170 – 172Combined sources3
Helixi173 – 178Combined sources6
Turni179 – 181Combined sources3
Beta strandi183 – 185Combined sources3
Beta strandi186 – 188Combined sources3
Beta strandi192 – 195Combined sources4
Beta strandi197 – 202Combined sources6
Beta strandi208 – 210Combined sources3
Beta strandi217 – 219Combined sources3
Beta strandi220 – 225Combined sources6
Beta strandi226 – 228Combined sources3
Turni229 – 231Combined sources3
Beta strandi239 – 243Combined sources5
Beta strandi246 – 249Combined sources4
Helixi253 – 276Combined sources24
Beta strandi280 – 283Combined sources4
Helixi287 – 298Combined sources12
Beta strandi299 – 301Combined sources3
Helixi306 – 309Combined sources4
Helixi310 – 313Combined sources4
Helixi325 – 328Combined sources4
Beta strandi334 – 338Combined sources5
Helixi340 – 359Combined sources20
Turni360 – 365Combined sources6
Helixi369 – 373Combined sources5
Helixi379 – 385Combined sources7
Beta strandi389 – 392Combined sources4
Helixi398 – 403Combined sources6
Beta strandi404 – 406Combined sources3
Beta strandi408 – 410Combined sources3
Beta strandi421 – 425Combined sources5
Helixi430 – 443Combined sources14
Beta strandi447 – 451Combined sources5
Helixi457 – 466Combined sources10
Turni467 – 469Combined sources3
Beta strandi472 – 475Combined sources4
Turni482 – 484Combined sources3
Helixi485 – 491Combined sources7
Turni492 – 494Combined sources3
Beta strandi497 – 502Combined sources6
Beta strandi506 – 508Combined sources3
Beta strandi514 – 521Combined sources8
Beta strandi522 – 524Combined sources3
Helixi527 – 529Combined sources3
Helixi531 – 538Combined sources8
Helixi539 – 541Combined sources3
Beta strandi548 – 552Combined sources5
Helixi558 – 580Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D9XX-ray2.60A2-658[»]
1D9ZX-ray3.15A2-658[»]
1T5LX-ray2.60A/B2-658[»]
2FDCX-ray3.30A/B2-658[»]
ProteinModelPortaliP56981.
SMRiP56981.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56981.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 414Helicase ATP-bindingAdd BLAST388
Domaini430 – 596Helicase C-terminalAdd BLAST167
Domaini622 – 657UVRAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi93 – 116Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.

Sequence similaritiesi

Belongs to the UvrB family.Curated
Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated
Contains 1 UVR domain.Curated

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEGRFQLVA PYEPQGDQPQ AIAKLVDGLR RGVKHQTLLG ATGTGKTFTI
60 70 80 90 100
SNVIAQVNKP TLVIAHNKTL AGQLYSELKE FFPHNAVEYF VSYYDYYQPE
110 120 130 140 150
AYVPQTDTYI EKDAKINDEI DKLRHSATSA LFERRDVIIV ASVSCIYGLG
160 170 180 190 200
SPEEYRELVV SLRVGMEIER NALLRRLVDI QYDRNDIDFR GTFRVRGDVV
210 220 230 240 250
EIFPASRDEH CIRVEFFGDE IERIREVDAL TGKVLGEREH VAIFPASHFV
260 270 280 290 300
TREEKMRLAI QNIEQELEER LAELRAQGKL LEAQRLEQRT RYDLEMMREM
310 320 330 340 350
GFCSGIENYS RHLALRPPGS TPYTLLDYFP DDFLIIVDES HVTLPQLRGM
360 370 380 390 400
YNGDRARKQV LVDHGFRLPS ALDNRPLTFE EFEQKINQII YVSATPGPYE
410 420 430 440 450
LEHSPGVVEQ IIRPTGLLDP TIDVRPTKGQ IDDLIGEIRE RVERNERTLV
460 470 480 490 500
TTLTKKMAED LTDYLKEAGI KVAYLHSEIK TLERIEIIRD LRLGKYDVLV
510 520 530 540 550
GINLLREGLD IPEVSLVAIL DADKEGFLRS ERSLIQTIGR AARNANGHVI
560 570 580 590 600
MYADTITKSM EIAIQETKRR RAIQEEYNRK HGIVPRTVKK EIRDVIRATY
610 620 630 640 650
AAEETEMYEA KPAAAMTKQE REELIRTLEA EMKEAAKALD FERAAQLRDI

IFELKAEG
Length:658
Mass (Da):75,455
Last modified:January 23, 2007 - v2
Checksum:iEEFCA1C76F56F826
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D9XX-ray2.60A2-658[»]
1D9ZX-ray3.15A2-658[»]
1T5LX-ray2.60A/B2-658[»]
2FDCX-ray3.30A/B2-658[»]
ProteinModelPortaliP56981.
SMRiP56981.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP56981.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVRB_BACCA
AccessioniPrimary (citable) accession number: P56981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.