Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Divisome-associated membrane protein Blr

Gene

blr

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cell division machinery, which is probably involved in the stabilization of the divisome under certain stress conditions.1 Publication

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to cell envelope stress Source: EcoCyc
  • response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Stress response

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1561.
ECOL316407:JW5963-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Divisome-associated membrane protein Blr
Alternative name(s):
Beta-lactam resistance protein
Gene namesi
Name:blr
Ordered Locus Names:b4409, JW5963
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14389. blr.

Subcellular locationi

  • Cell inner membrane 2 Publications; Single-pass membrane protein 2 Publications

  • Note: Localizes to the division septum. Localization requires FtsQ and FtsN.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 4117PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Inactivation increases the susceptibility of bacteria to a number of antibiotics that inhibit peptidoglycan biosynthesis. Increases the susceptibility to cycloserine, bacitracin, and a wide spectrum of beta-lactam antibiotics. Does not affect susceptibility to fosfomycin, valinomycin, tetracycline, chloramphenicol, gentamicin or quinolones.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4141Divisome-associated membrane protein BlrPRO_0000064943Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP56976.

Interactioni

Subunit structurei

Interacts with FtsL and several other divisomal proteins, including FtsI, FtsK, FtsN, FtsQ, FtsW and YmgF.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsLP0AEN43EBI-6419495,EBI-1119082
ftsNP291313EBI-6419495,EBI-1134233

Protein-protein interaction databases

BioGridi4259631. 432 interactions.
IntActiP56976. 8 interactions.
STRINGi511145.b4409.

Structurei

3D structure databases

ProteinModelPortaliP56976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106C6R. Bacteria.
ENOG410Y1ZA. LUCA.
HOGENOMiHOG000294500.
OrthoDBiEOG62ZJ1T.

Sequencei

Sequence statusi: Complete.

P56976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
MNRLIELTGW IVLVVSVILL GVASHIDNYQ PPEQSASVQH K
Length:41
Mass (Da):4,556
Last modified:December 1, 2000 - v1
Checksum:iD384EBB01276D46B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF219227 Genomic DNA. Translation: AAF82191.1.
U00096 Genomic DNA. Translation: AAT48132.1.
AP009048 Genomic DNA. Translation: BAE76483.1.
RefSeqiWP_001300888.1. NZ_LN832404.1.
YP_025303.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48132; AAT48132; b4409.
BAE76483; BAE76483; BAE76483.
GeneIDi2847682.
KEGGiecj:JW5963.
eco:b4409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF219227 Genomic DNA. Translation: AAF82191.1.
U00096 Genomic DNA. Translation: AAT48132.1.
AP009048 Genomic DNA. Translation: BAE76483.1.
RefSeqiWP_001300888.1. NZ_LN832404.1.
YP_025303.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP56976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259631. 432 interactions.
IntActiP56976. 8 interactions.
STRINGi511145.b4409.

Proteomic databases

PaxDbiP56976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48132; AAT48132; b4409.
BAE76483; BAE76483; BAE76483.
GeneIDi2847682.
KEGGiecj:JW5963.
eco:b4409.

Organism-specific databases

EcoGeneiEG14389. blr.

Phylogenomic databases

eggNOGiENOG4106C6R. Bacteria.
ENOG410Y1ZA. LUCA.
HOGENOMiHOG000294500.
OrthoDBiEOG62ZJ1T.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1561.
ECOL316407:JW5963-MONOMER.

Miscellaneous databases

PROiP56976.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "'Intergenic' blr gene in Escherichia coli encodes a 41-residue membrane protein affecting intrinsic susceptibility to certain inhibitors of peptidoglycan synthesis."
    Wong R.S.Y., McMurry L.M., Levy S.B.
    Mol. Microbiol. 37:364-370(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, SUBCELLULAR LOCATION, TOPOLOGY, DISRUPTION PHENOTYPE, BLOCKAGE OF N-TERMINUS.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The beta-lactam resistance protein Blr, a small membrane polypeptide, is a component of the Escherichia coli cell division machinery."
    Karimova G., Davi M., Ladant D.
    J. Bacteriol. 194:5576-5588(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FTSL; FTSI; FTSK; FTSN; FTSQ; FTSW AND YMGF, SUBCELLULAR LOCATION.
    Strain: K12.

Entry informationi

Entry nameiBLR_ECOLI
AccessioniPrimary (citable) accession number: P56976
Secondary accession number(s): Q2MB73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 20, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.