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Protein

Syntaxin-17

Gene

STX17

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane (PubMed:23217709, PubMed:25686604). May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi (PubMed:21545355).3 Publications

GO - Molecular functioni

  • protein phosphatase binding Source: UniProtKB
  • SNAP receptor activity Source: UniProtKB
  • SNARE binding Source: UniProtKB

GO - Biological processi

  • autophagosome maturation Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment organization Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi organization Source: UniProtKB
  • intracellular protein transport Source: GO_Central
  • protein localization to pre-autophagosomal structure Source: MGI
  • vesicle docking Source: GO_Central
  • vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136874-MONOMER.
ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-171 Publication
Gene namesi
Name:STX17Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:11432. STX17.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 228CytoplasmicSequence analysisAdd BLAST227
Transmembranei229 – 249HelicalSequence analysisAdd BLAST21
Topological domaini250 – 254LumenalSequence analysis5
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Topological domaini276 – 302CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

  • autophagosome membrane Source: UniProtKB
  • cytosol Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: UniProtKB
  • ER-mitochondrion membrane contact site Source: MGI
  • ER to Golgi transport vesicle Source: UniProtKB
  • ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: GO_Central
  • mitochondrion Source: UniProtKB
  • smooth endoplasmic reticulum membrane Source: UniProtKB
  • SNARE complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi244G → L: Alters localization to the autophagosome; when associated with Leu-248. 1 Publication1
Mutagenesisi248G → L: Alters localization to the autophagosome; when associated with Leu-244. 1 Publication1
Mutagenesisi264G → L: Alters localization to the autophagosome; when associated with Leu-268. 1 Publication1
Mutagenesisi268G → L: Alters localization to the autophagosome; when associated with Leu-264. 1 Publication1
Mutagenesisi299 – 300KK → AA: Localizes to the Golgi instead of the endoplasmic reticulum. 1 Publication2

Organism-specific databases

DisGeNETi55014.
OpenTargetsiENSG00000136874.
PharmGKBiPA36232.

Polymorphism and mutation databases

BioMutaiSTX17.
DMDMi212286190.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002102282 – 302Syntaxin-17Add BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei41N6-acetyllysineCombined sources1
Modified residuei157Phosphotyrosine; by ABL1By similarity1
Modified residuei289PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Tyr-157 probably by ABL1. Dephosphorylation by PTPN2; regulates exit from the endoplasmic reticulum (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP56962.
MaxQBiP56962.
PaxDbiP56962.
PeptideAtlasiP56962.
PRIDEiP56962.

PTM databases

iPTMnetiP56962.
PhosphoSitePlusiP56962.

Expressioni

Gene expression databases

BgeeiENSG00000136874.
CleanExiHS_STX17.
ExpressionAtlasiP56962. baseline and differential.
GenevisibleiP56962. HS.

Organism-specific databases

HPAiHPA001204.

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (PubMed:23217709, PubMed:25686604). Interacts with VAMP7 and VTI1B (PubMed:23217709). Probably interacts with BET1, SCFD1 and SEC22B (By similarity). Interacts with PTPN2 and ABL1; involved in STX17 phosphorylation (PubMed:23006999). Interacts with COPB1 (By similarity). Interacts with TMED9 and TMED10; the interaction is direct (PubMed:21545355). Interacts with ATG14 (PubMed:25686604).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Vamp7P702802EBI-2797775,EBI-6555653From a different organism.
VAMP8Q9BV408EBI-2797775,EBI-727028
VPS16Q9H2693EBI-2797775,EBI-2655929
VPS33AQ96AX14EBI-2797775,EBI-2527283

GO - Molecular functioni

  • protein phosphatase binding Source: UniProtKB
  • SNAP receptor activity Source: UniProtKB
  • SNARE binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120346. 10 interactors.
DIPiDIP-47297N.
IntActiP56962. 31 interactors.
STRINGi9606.ENSP00000259400.

Structurei

Secondary structure

1302
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi173 – 195Combined sources23
Helixi197 – 226Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WY4X-ray1.40B170-227[»]
ProteinModelPortaliP56962.
SMRiP56962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 224t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni229 – 275Necessary and sufficient for localization to autophagosome1 PublicationAdd BLAST47

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili53 – 123Sequence analysisAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi299 – 302Endoplasmic reticulum retention signalSequence analysis4

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
GeneTreeiENSGT00390000013283.
HOGENOMiHOG000006905.
HOVERGENiHBG055257.
InParanoidiP56962.
KOiK08491.
OMAiANMREME.
OrthoDBiEOG091G127M.
PhylomeDBiP56962.
TreeFamiTF323947.

Family and domain databases

InterProiIPR028676. STX17.
IPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF139. PTHR19957:SF139. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDEEKVKL RRLEPAIQKF IKIVIPTDLE RLRKHQINIE KYQRCRIWDK
60 70 80 90 100
LHEEHINAGR TVQQLRSNIR EIEKLCLKVR KDDLVLLKRM IDPVKEEASA
110 120 130 140 150
ATAEFLQLHL ESVEELKKQF NDEETLLQPP LTRSMTVGGA FHTTEAEASS
160 170 180 190 200
QSLTQIYALP EIPQDQNAAE SWETLEADLI ELSQLVTDFS LLVNSQQEKI
210 220 230 240 250
DSIADHVNSA AVNVEEGTKN LGKAAKYKLA ALPVAGALIG GMVGGPIGLL
260 270 280 290 300
AGFKVAGIAA ALGGGVLGFT GGKLIQRKKQ KMMEKLTSSC PDLPSQTDKK

CS
Length:302
Mass (Da):33,403
Last modified:July 22, 2008 - v2
Checksum:i51AD5427EFBAC541
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28D → N in BAA91311 (PubMed:14702039).Curated1
Sequence conflicti172W → R in BAA91311 (PubMed:14702039).Curated1
Sequence conflicti252G → C in BAA91311 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000658 mRNA. Translation: BAA91311.1.
AL358937 Genomic DNA. Translation: CAI95318.1.
CH471105 Genomic DNA. Translation: EAW58920.1.
BC101564 mRNA. Translation: AAI01565.1.
BC101566 mRNA. Translation: AAI01567.1.
CCDSiCCDS6745.1.
RefSeqiNP_060389.2. NM_017919.2.
XP_011517122.1. XM_011518820.2.
XP_011517123.1. XM_011518821.2.
UniGeneiHs.704031.

Genome annotation databases

EnsembliENST00000259400; ENSP00000259400; ENSG00000136874.
ENST00000525640; ENSP00000435981; ENSG00000136874.
ENST00000534052; ENSP00000433484; ENSG00000136874.
GeneIDi55014.
KEGGihsa:55014.
UCSCiuc004bal.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000658 mRNA. Translation: BAA91311.1.
AL358937 Genomic DNA. Translation: CAI95318.1.
CH471105 Genomic DNA. Translation: EAW58920.1.
BC101564 mRNA. Translation: AAI01565.1.
BC101566 mRNA. Translation: AAI01567.1.
CCDSiCCDS6745.1.
RefSeqiNP_060389.2. NM_017919.2.
XP_011517122.1. XM_011518820.2.
XP_011517123.1. XM_011518821.2.
UniGeneiHs.704031.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WY4X-ray1.40B170-227[»]
ProteinModelPortaliP56962.
SMRiP56962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120346. 10 interactors.
DIPiDIP-47297N.
IntActiP56962. 31 interactors.
STRINGi9606.ENSP00000259400.

PTM databases

iPTMnetiP56962.
PhosphoSitePlusiP56962.

Polymorphism and mutation databases

BioMutaiSTX17.
DMDMi212286190.

Proteomic databases

EPDiP56962.
MaxQBiP56962.
PaxDbiP56962.
PeptideAtlasiP56962.
PRIDEiP56962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000259400; ENSP00000259400; ENSG00000136874.
ENST00000525640; ENSP00000435981; ENSG00000136874.
ENST00000534052; ENSP00000433484; ENSG00000136874.
GeneIDi55014.
KEGGihsa:55014.
UCSCiuc004bal.5. human.

Organism-specific databases

CTDi55014.
DisGeNETi55014.
GeneCardsiSTX17.
H-InvDBHIX0008233.
HGNCiHGNC:11432. STX17.
HPAiHPA001204.
MIMi604204. gene.
neXtProtiNX_P56962.
OpenTargetsiENSG00000136874.
PharmGKBiPA36232.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
GeneTreeiENSGT00390000013283.
HOGENOMiHOG000006905.
HOVERGENiHBG055257.
InParanoidiP56962.
KOiK08491.
OMAiANMREME.
OrthoDBiEOG091G127M.
PhylomeDBiP56962.
TreeFamiTF323947.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136874-MONOMER.
ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Miscellaneous databases

ChiTaRSiSTX17. human.
GenomeRNAii55014.
PROiP56962.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136874.
CleanExiHS_STX17.
ExpressionAtlasiP56962. baseline and differential.
GenevisibleiP56962. HS.

Family and domain databases

InterProiIPR028676. STX17.
IPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF139. PTHR19957:SF139. 1 hit.
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX17_HUMAN
AccessioniPrimary (citable) accession number: P56962
Secondary accession number(s): Q4VXC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 22, 2008
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.