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Protein

Exosome component 10

Gene

Exosc10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA (By similarity).By similarity

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. exoribonuclease activity Source: MGI
  3. nucleotide binding Source: InterPro
  4. poly(A) RNA binding Source: MGI

GO - Biological processi

  1. CUT catabolic process Source: MGI
  2. dosage compensation by inactivation of X chromosome Source: MGI
  3. histone mRNA catabolic process Source: UniProtKB
  4. maturation of 5.8S rRNA Source: MGI
  5. nuclear mRNA surveillance Source: MGI
  6. nuclear polyadenylation-dependent rRNA catabolic process Source: MGI
  7. nuclear retention of unspliced pre-mRNA at the site of transcription Source: MGI
  8. nuclear-transcribed mRNA catabolic process Source: MGI
  9. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: MGI
  10. RNA phosphodiester bond hydrolysis, exonucleolytic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Nonsense-mediated mRNA decay, rRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Exosome component 10 (EC:3.1.13.-)
Alternative name(s):
Autoantigen PM/Scl 2 homolog
Polymyositis/scleroderma autoantigen 2 homolog
Gene namesi
Name:Exosc10
Synonyms:Pmscl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1355322. Exosc10.

Subcellular locationi

Cytoplasm By similarity. Nucleusnucleolus By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. exosome (RNase complex) Source: UniProtKB
  3. membrane Source: MGI
  4. nuclear exosome (RNase complex) Source: InterPro
  5. nucleolus Source: UniProtKB
  6. nucleoplasm Source: MGI
  7. nucleus Source: MGI
  8. transcriptionally active chromatin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Exosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Exosome component 10PRO_0000087134Add
BLAST

Proteomic databases

MaxQBiP56960.
PaxDbiP56960.
PRIDEiP56960.

PTM databases

PhosphoSiteiP56960.

Expressioni

Gene expression databases

BgeeiP56960.
CleanExiMM_EXOSC10.
ExpressionAtlasiP56960. baseline and differential.
GenevestigatoriP56960.

Interactioni

Subunit structurei

Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms. Interacts with C1D and MPHOSPH6 (By similarity). Interacts with ALYREF/THOC4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi206151. 1 interaction.
IntActiP56960. 1 interaction.
MINTiMINT-4094804.

Structurei

3D structure databases

ProteinModelPortaliP56960.
SMRiP56960. Positions 179-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini503 – 58381HRDCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HRDC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0349.
GeneTreeiENSGT00390000015408.
HOGENOMiHOG000001579.
HOVERGENiHBG051524.
InParanoidiP56960.
KOiK12591.
OMAiGNKSMSF.
OrthoDBiEOG7Z3F3S.
TreeFamiTF105991.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR012588. Exosome-assoc_fac_Rrp6_N.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01612. DNA_pol_A_exo1. 1 hit.
PF00570. HRDC. 1 hit.
PF08066. PMC2NT. 1 hit.
[Graphical view]
SMARTiSM00474. 35EXOc. 1 hit.
SM00341. HRDC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50967. HRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPSPREHQ SAPATSATKP DAEMVLPGFP DADSFVKFAL GSVVAVTKAS
60 70 80 90 100
GGLPQFGDEY DFYRSFPAFQ AFCETQGDRL LQCMSRVMQY HGCRSNIKDR
110 120 130 140 150
SKVTELEDKF DLLVDTNDVI LERVGMLLDE ASGVNKHQQP VLPAGLQVPK
160 170 180 190 200
TIVSSWNRKA GEYGKKAKSE TFRLLHAKNI VRPQLRFREK IDNSNTPFLP
210 220 230 240 250
KIFVKPNARK PLPLALSKER RERPQDRPED LDVPPALADF IHQQRTQQVE
260 270 280 290 300
QDMFAHPYQY ELDHFTPPQS VLQRPKPQLY RAVGETPCHL VSSLDELVEL
310 320 330 340 350
NEKLLGCQEF AVDLEHHSYR SFLGLTCLMQ ISTRTEDFIV DTLELRSDMY
360 370 380 390 400
ILNESLTDPA IVKVFHGADS DIEWLQKDFG LYVVNMFDTH QAARLLNLAR
410 420 430 440 450
HSLDHLLRLY CGVESNKQYQ LADWRIRPLP EEMLSYARDD THYLLYIYDR
460 470 480 490 500
MRLELWERGN HQPVQLQVVW QRSRDICLKK FVKPIFTDES YLELYRKQKK
510 520 530 540 550
HLNSQQLTAF QLLFAWRDKT ARREDESYGY VLPNHMMLKI AEELPKEPQG
560 570 580 590 600
IIACCNPVPP LVRQQINEMH LLIQQAREMP LLKSENAAGV RKSGPLPSAE
610 620 630 640 650
RLENDLFGPH DCSHAPPDNY QNTSTDGTLP LQKQPSLFTE GKEETSVDAG
660 670 680 690 700
CLLATAVITL FSEPNTEEGG KTPLTVAQKK AQNIMQSFEN PFRMFLPSLE
710 720 730 740 750
HKAHISQAAK FDPSSKIYEI SNRWKLASQV QVQKEPKEAT KKKVAEQTAA
760 770 780 790 800
REEAKEEAAA GVLEQAIPVR QQAALENATK KRERATSDLR TIEQKQEKKR
810 820 830 840 850
LKSSKKAKDP DPPGKDFSPY DYSQSDFRAF AGDSKSKPSS QFDPNKLAPS
860 870 880
GKKGVGAKKC KQSVGNKSMS FAVGKSDRGF RHNWPKR
Length:887
Mass (Da):100,942
Last modified:July 27, 2011 - v2
Checksum:i173164944163620C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti792 – 7921I → V in AAF01779 (PubMed:10708524).Curated
Sequence conflicti792 – 7921I → V in AAF01781 (PubMed:10708524).Curated
Sequence conflicti860 – 8601C → F in AAF01779 (PubMed:10708524).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091392 mRNA. Translation: AAF01779.1.
AF091505
, AF091506, AF092074, AF092075, AF092076, AF092077, AF092078, AF092079, AF092080, AF092081, AF092082, AF092083 Genomic DNA. Translation: AAF01781.1.
AL606969, AL713995 Genomic DNA. Translation: CAM21195.1.
AL713995, AL606969 Genomic DNA. Translation: CAM23945.1.
CCDSiCCDS18939.1.
RefSeqiNP_057908.2. NM_016699.2.
UniGeneiMm.266635.

Genome annotation databases

EnsembliENSMUST00000017408; ENSMUSP00000017408; ENSMUSG00000017264.
GeneIDi50912.
KEGGimmu:50912.
UCSCiuc008vut.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091392 mRNA. Translation: AAF01779.1.
AF091505
, AF091506, AF092074, AF092075, AF092076, AF092077, AF092078, AF092079, AF092080, AF092081, AF092082, AF092083 Genomic DNA. Translation: AAF01781.1.
AL606969, AL713995 Genomic DNA. Translation: CAM21195.1.
AL713995, AL606969 Genomic DNA. Translation: CAM23945.1.
CCDSiCCDS18939.1.
RefSeqiNP_057908.2. NM_016699.2.
UniGeneiMm.266635.

3D structure databases

ProteinModelPortaliP56960.
SMRiP56960. Positions 179-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206151. 1 interaction.
IntActiP56960. 1 interaction.
MINTiMINT-4094804.

PTM databases

PhosphoSiteiP56960.

Proteomic databases

MaxQBiP56960.
PaxDbiP56960.
PRIDEiP56960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017408; ENSMUSP00000017408; ENSMUSG00000017264.
GeneIDi50912.
KEGGimmu:50912.
UCSCiuc008vut.2. mouse.

Organism-specific databases

CTDi5394.
MGIiMGI:1355322. Exosc10.

Phylogenomic databases

eggNOGiCOG0349.
GeneTreeiENSGT00390000015408.
HOGENOMiHOG000001579.
HOVERGENiHBG051524.
InParanoidiP56960.
KOiK12591.
OMAiGNKSMSF.
OrthoDBiEOG7Z3F3S.
TreeFamiTF105991.

Miscellaneous databases

ChiTaRSiExosc10. mouse.
NextBioi307899.
PROiP56960.
SOURCEiSearch...

Gene expression databases

BgeeiP56960.
CleanExiMM_EXOSC10.
ExpressionAtlasiP56960. baseline and differential.
GenevestigatoriP56960.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR012588. Exosome-assoc_fac_Rrp6_N.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01612. DNA_pol_A_exo1. 1 hit.
PF00570. HRDC. 1 hit.
PF08066. PMC2NT. 1 hit.
[Graphical view]
SMARTiSM00474. 35EXOc. 1 hit.
SM00341. HRDC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and localization of mouse Pmscl1 and Pmscl2 genes."
    Bliskovski V., Liddell R., Ramsay E.S., Miller M.J., Mock B.A.
    Genomics 64:106-110(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ and BALB/c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 395-400, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiEXOSX_MOUSE
AccessioniPrimary (citable) accession number: P56960
Secondary accession number(s): B1ARY9, Q9QYS8, Q9R0B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.