P56958 (RNB_HHV1M) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein

Alternative name(s):
Vmw21

Gene names

Name:

US11

Organism

Human herpesvirus 1 (strain MP) (HHV-1) (Human herpes simplex virus 1)

Taxonomic identifier

10307 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Binds DNA and RNA. Is one of the most abundant viral proteins present in cells late in infection. Not necessary for virus viability. Component of the HSV-1 virion, with approximately 600 to 1000 copies per virion. Binds specifically to UL34 mRNA (in vitro) and seems to regulate the expression of that gene By similarity.
Subunit structure	Associates with RNA derived from the 60S ribosomal subunits. Seems to form large heterogeneous polymers of up to 200 identical subunits in the cytoplasm By similarity.
Subcellular location	Host nucleus › host nucleolus By similarity. Host cytoplasm By similarity. Note: Following infection, it is released into the cell cytoplasm By similarity.
Domain	The first 40 amino acids of the N-terminal region is an effector domain necessary for the transactivation of HTLV-1 env gene expression, which may interact with cellular proteins By similarity. The N-terminal tetrapeptide may be responsible for virion incorporation By similarity. The C-terminal half, rich in Arg and Pro residues, seems to be responsible for the RNA-binding activity, and for the association with ribosomes and the localization to the nucleolus. This region may adopt a poly-L-proline II helix secondary structure By similarity.
Post-translational modification	May be phosphorylated on Ser residues by host kinases By similarity.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host PKR by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral immunoevasion
Cellular component	Host cytoplasm Host nucleus
Developmental stage	Late protein
Domain	Repeat
Ligand	DNA-binding RNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	suppression by virus of host PKR activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	RNA-binding protein		PRO_0000115741
Regions
	Repeat	85 – 90	6	1
	Repeat	91 – 96	6	2
	Repeat	97 – 102	6	3
	Repeat	103 – 108	6	4
	Repeat	109 – 114	6	5
	Repeat	115 – 120	6	6
	Repeat	121 – 126	6	7
	Repeat	127 – 132	6	8
	Repeat	133 – 138	6	9
	Repeat	139 – 144	6	10
	Repeat	145 – 150	6	11
	Repeat	151 – 156	6	12
	Region	85 – 156	72	12 X 6 AA approximate tandem repeats

Sequences

	Sequence		Length	Mass (Da)
	P56958 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 1CF68615474462FF	FASTA	161	17,745
	10 20 30 40 50 60 MSQTQPPAPV GPGDPDVYLK GVPSAGMHPR GVHAPRGHPR MISGPPQRGD NDQAAGQCGD 70 80 90 100 110 120 SGLLRVGADT TISKPSEAVR PPTIPRTPRV PREPRVPRPP REPREPRVPR ASRDPRVPRD 130 140 150 160 PRDPRQPRSP REPRSPREPR PPREPRTPRT TREPRTARGA V

References

[1]	Diaz J.-J. Submitted (JUN-2000) to UniProtKB Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020124. Herpes_US11_RNA-bd_N. [Graphical view]
ProDom	PD028529. Herpes_US11_RNA-bd_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RNB_HHV1M

Accession

Primary (citable) accession number: P56958

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	October 3, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program