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Protein

Pro-MCH

Gene

Pmch

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal.1 Publication

GO - Molecular functioni

  • melanin-concentrating hormone activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • feeding behavior Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB-KW
  • synaptic transmission Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Neuropeptide

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-MCH
Cleaved into the following 3 chains:
Neuropeptide-glycine-glutamic acid
Short name:
NGE
Short name:
Neuropeptide G-E
Neuropeptide-glutamic acid-isoleucine
Short name:
NEI
Short name:
Neuropeptide E-I
Gene namesi
Name:Pmch
Synonyms:Mch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97629. Pmch.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 165144Pro-MCHPRO_0000019112Add
BLAST
Peptidei110 – 12819Neuropeptide-glycine-glutamic acidSequence analysisPRO_0000019113Add
BLAST
Peptidei131 – 14313Neuropeptide-glutamic acid-isoleucineBy similarityPRO_0000019114Add
BLAST
Peptidei147 – 16519Melanin-concentrating hormonePRO_0000019115Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431Isoleucine amideBy similarity
Disulfide bondi153 ↔ 162By similarity

Post-translational modificationi

Pro-MCH is processed differentially in the brain and in peripheral organs producing two neuropeptides; NEI and MCH. A third peptide, NGE, may also be produced. Preferential processing in neurons by prohormone convertase 2 (PC2) generates NEI. MCH is generated in neurons of the lateral hypothalmic area by several prohormone convertases including PC1/3, PC2 and PC5/6.1 Publication

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP56942.
PRIDEiP56942.

Expressioni

Tissue specificityi

Predominantly expressed in hypothalamus. Also found in heart, intestine, spleen and testis (spermatogonia, early spermatocytes and Sertoli cells). In brain only mature MCH and NEI peptides are present. In peripheral tissues a large product, encompassing the NEI and MCH domains of the precursor, is found predominantly.1 Publication

Developmental stagei

Expression is enhanced between postnatal days 10 and 15.

Gene expression databases

CleanExiMM_PMCH.
GenevisibleiP56942. MM.

Interactioni

GO - Molecular functioni

  • melanin-concentrating hormone activity Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044352.

Family & Domainsi

Sequence similaritiesi

Belongs to the MCH family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IW1Y. Eukaryota.
ENOG410XWB7. LUCA.
GeneTreeiENSGT00390000004984.
HOGENOMiHOG000230520.
HOVERGENiHBG052429.
InParanoidiP56942.
KOiK05229.
OMAiFMNDEEN.
OrthoDBiEOG7X6M1T.
TreeFamiTF330944.

Family and domain databases

InterProiIPR005456. Prepro-melanin_conc_hormone.
[Graphical view]
PANTHERiPTHR12091. PTHR12091. 1 hit.
PfamiPF05824. Pro-MCH. 1 hit.
[Graphical view]
PRINTSiPR01641. PROMCHFAMILY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKMTLSSYM LMLAFSLFSQ GILLSASKSI RNLEDDIVFN TFRMGKAFQK
60 70 80 90 100
EDTAERSVVA PSLEQYKNDE SGFMNDDDNK NSKNTGSKQN LVTHGLPLSL
110 120 130 140 150
AVKPYLALKG SVAFPAENGV QNAESTQEKR EIGDEENSAK FPIGRRDFDM
160
LRCMLGRVYR PCWQV
Length:165
Mass (Da):18,516
Last modified:June 26, 2013 - v2
Checksum:i1872B6B1D4BEEAC2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131A → AV no nucleotide entry (Ref. 1) Curated
Sequence conflicti123 – 1231A → T no nucleotide entry (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020723 mRNA. Translation: BAB32189.1.
AK138363 mRNA. Translation: BAE23633.1.
AC139754 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21464.1.
CCDSiCCDS24105.1.
RefSeqiNP_084247.1. NM_029971.2.
UniGeneiMm.391436.

Genome annotation databases

EnsembliENSMUST00000048621; ENSMUSP00000044352; ENSMUSG00000035383.
GeneIDi110312.
KEGGimmu:110312.
UCSCiuc007grc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK020723 mRNA. Translation: BAB32189.1.
AK138363 mRNA. Translation: BAE23633.1.
AC139754 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21464.1.
CCDSiCCDS24105.1.
RefSeqiNP_084247.1. NM_029971.2.
UniGeneiMm.391436.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044352.

Proteomic databases

PaxDbiP56942.
PRIDEiP56942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048621; ENSMUSP00000044352; ENSMUSG00000035383.
GeneIDi110312.
KEGGimmu:110312.
UCSCiuc007grc.1. mouse.

Organism-specific databases

CTDi5367.
MGIiMGI:97629. Pmch.

Phylogenomic databases

eggNOGiENOG410IW1Y. Eukaryota.
ENOG410XWB7. LUCA.
GeneTreeiENSGT00390000004984.
HOGENOMiHOG000230520.
HOVERGENiHBG052429.
InParanoidiP56942.
KOiK05229.
OMAiFMNDEEN.
OrthoDBiEOG7X6M1T.
TreeFamiTF330944.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP56942.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PMCH.
GenevisibleiP56942. MM.

Family and domain databases

InterProiIPR005456. Prepro-melanin_conc_hormone.
[Graphical view]
PANTHERiPTHR12091. PTHR12091. 1 hit.
PfamiPF05824. Pro-MCH. 1 hit.
[Graphical view]
PRINTSiPR01641. PROMCHFAMILY.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and regulation of the mouse melanin-concentrating hormone mRNA and gene."
    Breton C., Presse F., Hervieu G., Nahon J.-L.
    Mol. Cell. Neurosci. 4:271-284(1993)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hypothalamus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Developmental and stage-dependent expression of melanin-concentrating hormone in mammalian germ cells."
    Hervieu G., Segretain D., Nahon J.-L.
    Biol. Reprod. 54:1161-1172(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "A role for melanin-concentrating hormone in the central regulation of feeding behaviour."
    Qu D., Ludwig D.S., Gammeltoft S., Piper M., Pelleymounter M.A., Cullen M.J., Mathes W.F., Przypek R., Kanarek R., Maratos-Flier E.
    Nature 380:243-247(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Cellular localization and role of prohormone convertases in the processing of pro-melanin concentrating hormone in mammals."
    Viale A., Ortola C., Hervieu G., Furuta M., Barbero P., Steiner D.F., Seidah N.G., Nahon J.-L.
    J. Biol. Chem. 274:6536-6545(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING.

Entry informationi

Entry nameiMCH_MOUSE
AccessioniPrimary (citable) accession number: P56942
Secondary accession number(s): Q9D220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 26, 2013
Last modified: June 8, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.