Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform

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P56932 (2ABD_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform

Alternative name(s):
PP2A subunit B isoform B55-delta
PP2A subunit B isoform PR55-delta
PP2A subunit B isoform R2-delta
PP2A subunit B isoform delta

Gene names

Name:

Ppp2r2d

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment By similarity.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with ENSA (when phosphorylated at 'Ser-67') and ARPP19 (when phosphorylated at 'Ser-62'), leading to inhibit PP2A activity By similarity.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Widely expressed with high levels in brain, heart, placenta, skeletal muscle, testis, thymus and spleen.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Traceable author statement Ref.1. Source: RGD nucleoplasm Traceable author statement. Source: Reactome protein phosphatase type 2A complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	phosphatidate phosphatase activity Traceable author statement. Source: Reactome protein phosphatase type 2A regulator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 453

453

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform

PRO_0000071435

Regions

Repeat

32 – 71

WD 1

Repeat

97 – 138

WD 2

Repeat

181 – 219

WD 3

Repeat

230 – 270

WD 4

Repeat

289 – 327

WD 5

Repeat

344 – 385

WD 6

Repeat

420 – 452

WD 7

Compositional bias

3 – 8

Poly-Gly

Experimental info

Sequence conflict

330

H → Q in AAH81720. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P56932 [UniParc].

Last modified May 30, 2000. Version 1.
Checksum: 733E80A93A5BC2BB
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FASTA

453

51,982

        10         20         30         40         50         60 
MAGAGGGGCP TGGNDFQWCF SQVKGAVDED VAEADIISTV EFNYSGDLLA TGDKGGRVVI 

        70         80         90        100        110        120 
FQREQENKGR AHSRGEYNVY STFQSHEPEF DYLKSLEIEE KINKIRWLPQ QNAAHFLLST 

       130        140        150        160        170        180 
NDKTIKLWKI SERDKRAEGY NLKDEDGRLR DPFRITALRV PILKPMDLMV EASPRRIFAN 

       190        200        210        220        230        240 
AHTYHINSIS VNSDHETYLS ADDLRINLWH LEITDRSFNI VDIKPANMEE LTEVITAAEF 

       250        260        270        280        290        300 
HPHQCNVFVY SSSKGTIRLC DMRSSALCDR HAKFFEEPED PSSRSFFSEI ISSISDVKFS 

       310        320        330        340        350        360 
HSGRYMMTRD YLSVKVWDLN MEGRPVETHH VHEYLRSKLC SLYENDCIFD KFECCWNGSD 

       370        380        390        400        410        420 
SAIMTGSYNN FFRMFDRNTR RDVTLEASRE NSKPRASLKP RKVCSGGKRK KDEISVDSLD 

       430        440        450 
FNKKILHTAW HPMESIIAVA ATNNLYIFQD KIN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A." Strack S., Chang D., Zaucha J.A., Colbran R.J., Wadzinski B.E. FEBS Lett. 460:462-466(1999) [PubMed: 10556517] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF180350 mRNA. Translation: AAF08536.1. BC081720 mRNA. Translation: AAH81720.1.
IPI	IPI00197077.
RefSeq	NP_653347.2. NM_144746.2.
UniGene	Rn.116122.
3D structure databases
ProteinModelPortal	P56932.
ModBase	Search...
Protein-protein interaction databases
STRING	P56932.
Proteomic databases
PRIDE	P56932.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023139; ENSRNOP00000023139; ENSRNOG00000016940.
GeneID	246255.
KEGG	rno:246255.
UCSC	NM_144746. rat.
Organism-specific databases
CTD	55844.
RGD	708356. Ppp2r2d.
Phylogenomic databases
eggNOG	roNOG04944.
GeneTree	ENSGT00390000006311.
HOVERGEN	HBG000012.
InParanoid	P56932.
OrthoDB	EOG4M0F1K.
PhylomeDB	P56932.
Enzyme and pathway databases
Reactome	REACT_113568. Metabolism. REACT_33953. Metabolism of carbohydrates.
Gene expression databases
ArrayExpress	P56932.
Genevestigator	P56932.
GermOnline	ENSRNOG00000016940. Rattus norvegicus.
Family and domain databases
InterPro	IPR000009. PP2A_PR55. IPR018067. PP2A_PR55_CS. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
KO	K04354.
PANTHER	PTHR11871. Pp2A_PR55. 1 hit.
Pfam	PF00400. WD40. 1 hit. [Graphical view]
PIRSF	PIRSF037309. PP2A_PR55. 1 hit.
PRINTS	PR00600. PP2APR55.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS01024. PR55_1. 1 hit. PS01025. PR55_2. 1 hit. PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	623603.

Entry information

Entry name

2ABD_RAT

Accession

Primary (citable) accession number: P56932
Secondary accession number(s): Q66HR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents