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Protein

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform

Gene

Ppp2r2d

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-163685. Integration of energy metabolism.
R-RNO-163767. PP2A-mediated dephosphorylation of key metabolic factors.
R-RNO-70171. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform
Alternative name(s):
PP2A subunit B isoform B55-delta
PP2A subunit B isoform PR55-delta
PP2A subunit B isoform R2-delta
PP2A subunit B isoform delta
Gene namesi
Name:Ppp2r2d
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708356. Ppp2r2d.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleoplasm Source: Reactome
  • protein phosphatase type 2A complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoformPRO_0000071435Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei285 – 2851PhosphoserineBy similarity
Modified residuei305 – 3051PhosphotyrosineBy similarity
Modified residuei308 – 3081PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP56932.
PRIDEiP56932.

Expressioni

Tissue specificityi

Widely expressed with high levels in brain, heart, placenta, skeletal muscle, testis, thymus and spleen.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with ENSA (when phosphorylated at 'Ser-67') and ARPP19 (when phosphorylated at 'Ser-62'), leading to inhibit PP2A activity (By similarity).By similarity

Protein-protein interaction databases

IntActiP56932. 1 interaction.
MINTiMINT-4568514.
STRINGi10116.ENSRNOP00000023139.

Structurei

3D structure databases

ProteinModelPortaliP56932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati32 – 7140WD 1Add
BLAST
Repeati97 – 13842WD 2Add
BLAST
Repeati181 – 21939WD 3Add
BLAST
Repeati230 – 27041WD 4Add
BLAST
Repeati289 – 32739WD 5Add
BLAST
Repeati344 – 38542WD 6Add
BLAST
Repeati420 – 45233WD 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 86Poly-Gly

Sequence similaritiesi

Contains 7 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1354. Eukaryota.
COG5170. LUCA.
HOGENOMiHOG000089745.
HOVERGENiHBG000012.
InParanoidiP56932.
KOiK04354.
OrthoDBiEOG7Q5HCZ.
PhylomeDBiP56932.
TreeFamiTF105553.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR11871. PTHR11871. 1 hit.
PIRSFiPIRSF037309. PP2A_PR55. 1 hit.
PRINTSiPR00600. PP2APR55.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGAGGGGCP TGGNDFQWCF SQVKGAVDED VAEADIISTV EFNYSGDLLA
60 70 80 90 100
TGDKGGRVVI FQREQENKGR AHSRGEYNVY STFQSHEPEF DYLKSLEIEE
110 120 130 140 150
KINKIRWLPQ QNAAHFLLST NDKTIKLWKI SERDKRAEGY NLKDEDGRLR
160 170 180 190 200
DPFRITALRV PILKPMDLMV EASPRRIFAN AHTYHINSIS VNSDHETYLS
210 220 230 240 250
ADDLRINLWH LEITDRSFNI VDIKPANMEE LTEVITAAEF HPHQCNVFVY
260 270 280 290 300
SSSKGTIRLC DMRSSALCDR HAKFFEEPED PSSRSFFSEI ISSISDVKFS
310 320 330 340 350
HSGRYMMTRD YLSVKVWDLN MEGRPVETHH VHEYLRSKLC SLYENDCIFD
360 370 380 390 400
KFECCWNGSD SAIMTGSYNN FFRMFDRNTR RDVTLEASRE NSKPRASLKP
410 420 430 440 450
RKVCSGGKRK KDEISVDSLD FNKKILHTAW HPMESIIAVA ATNNLYIFQD

KIN
Length:453
Mass (Da):51,982
Last modified:May 30, 2000 - v1
Checksum:i733E80A93A5BC2BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti330 – 3301H → Q in AAH81720 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180350 mRNA. Translation: AAF08536.1.
BC081720 mRNA. Translation: AAH81720.1.
RefSeqiNP_653347.2. NM_144746.2.
UniGeneiRn.116122.

Genome annotation databases

GeneIDi246255.
KEGGirno:246255.
UCSCiRGD:708356. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180350 mRNA. Translation: AAF08536.1.
BC081720 mRNA. Translation: AAH81720.1.
RefSeqiNP_653347.2. NM_144746.2.
UniGeneiRn.116122.

3D structure databases

ProteinModelPortaliP56932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56932. 1 interaction.
MINTiMINT-4568514.
STRINGi10116.ENSRNOP00000023139.

Proteomic databases

PaxDbiP56932.
PRIDEiP56932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246255.
KEGGirno:246255.
UCSCiRGD:708356. rat.

Organism-specific databases

CTDi55844.
RGDi708356. Ppp2r2d.

Phylogenomic databases

eggNOGiKOG1354. Eukaryota.
COG5170. LUCA.
HOGENOMiHOG000089745.
HOVERGENiHBG000012.
InParanoidiP56932.
KOiK04354.
OrthoDBiEOG7Q5HCZ.
PhylomeDBiP56932.
TreeFamiTF105553.

Enzyme and pathway databases

ReactomeiR-RNO-163685. Integration of energy metabolism.
R-RNO-163767. PP2A-mediated dephosphorylation of key metabolic factors.
R-RNO-70171. Glycolysis.

Miscellaneous databases

PROiP56932.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR000009. PP2A_PR55.
IPR018067. PP2A_PR55_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR11871. PTHR11871. 1 hit.
PIRSFiPIRSF037309. PP2A_PR55. 1 hit.
PRINTSiPR00600. PP2APR55.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS01024. PR55_1. 1 hit.
PS01025. PR55_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of B delta, a novel regulatory subunit of protein phosphatase 2A."
    Strack S., Chang D., Zaucha J.A., Colbran R.J., Wadzinski B.E.
    FEBS Lett. 460:462-466(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry namei2ABD_RAT
AccessioniPrimary (citable) accession number: P56932
Secondary accession number(s): Q66HR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.