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P56931 (E2F2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor E2F2

Short name=E2F-2
Gene names
Name:E2f2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Subunit structure

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP.

Subcellular location

Nucleus.

Developmental stage

Expressed in the developing epidermis and intestinal epithelium. First detected in the epidermis at stage 13.5-14.5 dpc with higher levels in the head and thorax regions. At 15.5 dpc, expression is found in both the epithelium and, to a lesser extent in the underlying mesenchyme. At day 16.5 dpc, high expression in the basal cells. Later expression is found in the developing hair follicles, around the dermal papillae. In the developing intestinal epithelium, expression first observed around 14.5 dpc. Levels continue to increase at least until 19.5 dpc, with highest levels in the intervillus epithelium and in the bottom half of the villi. In the nervous system, first expressed at 9.5 dpc, in the forebrain. At 10.5 dpc, expressed broadly in the brain, and at lower levels in the upper regions of the spinal cord. By 11.5 dpc, E2F2 expression is found throughout the central nervous system and levels peak at 12.5-15.5 dpc. In the developing spinal cord, E2F2 expression found only in the dorsal region. In the developing retina, highest expression found in the 14.5-18.5 dpc embryonic retinoblastic cell layer. In other developing tissues, E2F2 is found highest in thymus and liver, with lower expression in lung, heart, kidney and skeletal muscle. Also found in choroid plexus and chondrocytes. Ref.4 Ref.5

Post-translational modification

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase By similarity.

Sequence similarities

Belongs to the E2F/DP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Transcription factor E2F2
PRO_0000219465

Regions

DNA binding109 – 19890 Potential
Region67 – 10741Cyclin A/CDK2 binding Potential
Region157 – 17822Leucine-zipper
Region199 – 29193Dimerization Potential
Region361 – 44383Transactivation Potential
Region416 – 43318Retinoblastoma protein binding Potential
Motif162 – 19837DEF box

Sequences

Sequence LengthMass (Da)Tools
P56931 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: DFC8158E5A8254F0

FASTA44348,499
        10         20         30         40         50         60 
MLRAPRTLAP ATAQPTKSLP ALNPTELWPS GLSSPQLCPA TTATTYYTSL YTQTVPSSVA 

        70         80         90        100        110        120 
LGTCLDATPH GPEGQIVRCA PAGRLPAKRK LDLEGIGRPT VPEFRTPKGK CIRVDGLPSP 

       130        140        150        160        170        180 
KTPKSPGEKT RYDTSLGLLT KKFIYLLSES EDGVLDLNWA AEVLDVQKRR IYDITNVLEG 

       190        200        210        220        230        240 
IQLIRKKSKN NIQWVGRELF EDPTRPSRQQ QLGQELKELM NAEQTLDQLI QSCSLSFKHL 

       250        260        270        280        290        300 
TEDNANKKLA YVTYQDIRAV GNFKEQTVIA VKAPPQTRLE VPDRAEENLQ IYLKSTQGPI 

       310        320        330        340        350        360 
EVYLCPEEGQ EPDSPAKEAL PSTSALSPIP DCAQPGCSTD SGIAETIEPS VLIPQPIPPP 

       370        380        390        400        410        420 
PPPPLPPAPS LVPLEATDNM LELSHPLLQQ TEDQFLSPIL AANSPLISFS PPLDQDEYLW 

       430        440 
GMDEGEGISD LFDSYDLGDL LIN 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Eye.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Expression patterns of the E2F family of transcription factors during murine epithelial development."
Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE OF 49-254, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[5]"Expression patterns of the E2F family of transcription factors during mouse nervous system development."
Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EAPP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK087452 mRNA. Translation: BAC39881.1.
AL935264 Genomic DNA. Translation: CAM13832.1.
BC062101 mRNA. Translation: AAH62101.1.
CCDSCCDS18801.1.
RefSeqNP_808401.1. NM_177733.6.
UniGeneMm.307932.

3D structure databases

ProteinModelPortalP56931.
SMRP56931. Positions 130-194, 208-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid232447. 3 interactions.
DIPDIP-48416N.
IntActP56931. 1 interaction.
MINTMINT-4110723.

PTM databases

PhosphoSiteP56931.

Proteomic databases

PaxDbP56931.
PRIDEP56931.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000061721; ENSMUSP00000050047; ENSMUSG00000018983.
GeneID242705.
KEGGmmu:242705.
UCSCuc008vht.2. mouse.

Organism-specific databases

CTD1870.
MGIMGI:1096341. E2f2.

Phylogenomic databases

eggNOGNOG289227.
GeneTreeENSGT00550000074403.
HOGENOMHOG000232045.
HOVERGENHBG002227.
InParanoidA2AW43.
KOK09389.
OMAKELMSME.
OrthoDBEOG738058.
PhylomeDBP56931.
TreeFamTF105566.

Gene expression databases

BgeeP56931.
CleanExMM_E2F2.
GenevestigatorP56931.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR12081. PTHR12081. 1 hit.
PfamPF02319. E2F_TDP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSE2f2. mouse.
NextBio385512.
PROP56931.
SOURCESearch...

Entry information

Entry nameE2F2_MOUSE
AccessionPrimary (citable) accession number: P56931
Secondary accession number(s): A2AW43, Q8BID0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot