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P56931

- E2F2_MOUSE

UniProt

P56931 - E2F2_MOUSE

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Protein
Transcription factor E2F2
Gene
E2f2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi109 – 19890 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. core promoter binding Source: Ensembl
  2. protein binding Source: MGI
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  3. lens fiber cell apoptotic process Source: MGI
  4. regulation of cell cycle Source: MGI
  5. regulation of transcription, DNA-templated Source: MGI
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F2
Short name:
E2F-2
Gene namesi
Name:E2f2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1096341. E2f2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
  2. transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Transcription factor E2F2
PRO_0000219465Add
BLAST

Post-translational modificationi

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP56931.
PRIDEiP56931.

PTM databases

PhosphoSiteiP56931.

Expressioni

Developmental stagei

Expressed in the developing epidermis and intestinal epithelium. First detected in the epidermis at stage 13.5-14.5 dpc with higher levels in the head and thorax regions. At 15.5 dpc, expression is found in both the epithelium and, to a lesser extent in the underlying mesenchyme. At day 16.5 dpc, high expression in the basal cells. Later expression is found in the developing hair follicles, around the dermal papillae. In the developing intestinal epithelium, expression first observed around 14.5 dpc. Levels continue to increase at least until 19.5 dpc, with highest levels in the intervillus epithelium and in the bottom half of the villi. In the nervous system, first expressed at 9.5 dpc, in the forebrain. At 10.5 dpc, expressed broadly in the brain, and at lower levels in the upper regions of the spinal cord. By 11.5 dpc, E2F2 expression is found throughout the central nervous system and levels peak at 12.5-15.5 dpc. In the developing spinal cord, E2F2 expression found only in the dorsal region. In the developing retina, highest expression found in the 14.5-18.5 dpc embryonic retinoblastic cell layer. In other developing tissues, E2F2 is found highest in thymus and liver, with lower expression in lung, heart, kidney and skeletal muscle. Also found in choroid plexus and chondrocytes.2 Publications

Gene expression databases

BgeeiP56931.
CleanExiMM_E2F2.
GenevestigatoriP56931.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP.

Protein-protein interaction databases

BioGridi232447. 3 interactions.
DIPiDIP-48416N.
IntActiP56931. 1 interaction.
MINTiMINT-4110723.

Structurei

3D structure databases

ProteinModelPortaliP56931.
SMRiP56931. Positions 130-194, 208-308.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 10741Cyclin A/CDK2 binding Reviewed prediction
Add
BLAST
Regioni157 – 17822Leucine-zipper
Add
BLAST
Regioni199 – 29193Dimerization Reviewed prediction
Add
BLAST
Regioni361 – 44383Transactivation Reviewed prediction
Add
BLAST
Regioni416 – 43318Retinoblastoma protein binding Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi162 – 19837DEF box
Add
BLAST

Sequence similaritiesi

Belongs to the E2F/DP family.

Phylogenomic databases

eggNOGiNOG289227.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiA2AW43.
KOiK09389.
OMAiKELMSME.
OrthoDBiEOG738058.
PhylomeDBiP56931.
TreeFamiTF105566.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56931-1 [UniParc]FASTAAdd to Basket

« Hide

MLRAPRTLAP ATAQPTKSLP ALNPTELWPS GLSSPQLCPA TTATTYYTSL    50
YTQTVPSSVA LGTCLDATPH GPEGQIVRCA PAGRLPAKRK LDLEGIGRPT 100
VPEFRTPKGK CIRVDGLPSP KTPKSPGEKT RYDTSLGLLT KKFIYLLSES 150
EDGVLDLNWA AEVLDVQKRR IYDITNVLEG IQLIRKKSKN NIQWVGRELF 200
EDPTRPSRQQ QLGQELKELM NAEQTLDQLI QSCSLSFKHL TEDNANKKLA 250
YVTYQDIRAV GNFKEQTVIA VKAPPQTRLE VPDRAEENLQ IYLKSTQGPI 300
EVYLCPEEGQ EPDSPAKEAL PSTSALSPIP DCAQPGCSTD SGIAETIEPS 350
VLIPQPIPPP PPPPLPPAPS LVPLEATDNM LELSHPLLQQ TEDQFLSPIL 400
AANSPLISFS PPLDQDEYLW GMDEGEGISD LFDSYDLGDL LIN 443
Length:443
Mass (Da):48,499
Last modified:August 30, 2005 - v2
Checksum:iDFC8158E5A8254F0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK087452 mRNA. Translation: BAC39881.1.
AL935264 Genomic DNA. Translation: CAM13832.1.
BC062101 mRNA. Translation: AAH62101.1.
CCDSiCCDS18801.1.
RefSeqiNP_808401.1. NM_177733.6.
UniGeneiMm.307932.

Genome annotation databases

EnsembliENSMUST00000061721; ENSMUSP00000050047; ENSMUSG00000018983.
GeneIDi242705.
KEGGimmu:242705.
UCSCiuc008vht.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK087452 mRNA. Translation: BAC39881.1 .
AL935264 Genomic DNA. Translation: CAM13832.1 .
BC062101 mRNA. Translation: AAH62101.1 .
CCDSi CCDS18801.1.
RefSeqi NP_808401.1. NM_177733.6.
UniGenei Mm.307932.

3D structure databases

ProteinModelPortali P56931.
SMRi P56931. Positions 130-194, 208-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 232447. 3 interactions.
DIPi DIP-48416N.
IntActi P56931. 1 interaction.
MINTi MINT-4110723.

PTM databases

PhosphoSitei P56931.

Proteomic databases

PaxDbi P56931.
PRIDEi P56931.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000061721 ; ENSMUSP00000050047 ; ENSMUSG00000018983 .
GeneIDi 242705.
KEGGi mmu:242705.
UCSCi uc008vht.2. mouse.

Organism-specific databases

CTDi 1870.
MGIi MGI:1096341. E2f2.

Phylogenomic databases

eggNOGi NOG289227.
GeneTreei ENSGT00550000074403.
HOGENOMi HOG000232045.
HOVERGENi HBG002227.
InParanoidi A2AW43.
KOi K09389.
OMAi KELMSME.
OrthoDBi EOG738058.
PhylomeDBi P56931.
TreeFami TF105566.

Miscellaneous databases

ChiTaRSi E2f2. mouse.
NextBioi 385512.
PROi P56931.
SOURCEi Search...

Gene expression databases

Bgeei P56931.
CleanExi MM_E2F2.
Genevestigatori P56931.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR12081. PTHR12081. 1 hit.
Pfami PF02319. E2F_TDP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE OF 49-254, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  5. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
    Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
    Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
    Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
    Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EAPP.

Entry informationi

Entry nameiE2F2_MOUSE
AccessioniPrimary (citable) accession number: P56931
Secondary accession number(s): A2AW43, Q8BID0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi