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P56931

- E2F2_MOUSE

UniProt

P56931 - E2F2_MOUSE

Protein

Transcription factor E2F2

Gene

E2f2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi109 – 19890Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: Ensembl
    2. protein binding Source: MGI
    3. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
    3. lens fiber cell apoptotic process Source: MGI
    4. regulation of cell cycle Source: MGI
    5. regulation of transcription, DNA-templated Source: MGI
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor E2F2
    Short name:
    E2F-2
    Gene namesi
    Name:E2f2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1096341. E2f2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: MGI
    2. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Transcription factor E2F2PRO_0000219465Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP56931.
    PRIDEiP56931.

    PTM databases

    PhosphoSiteiP56931.

    Expressioni

    Developmental stagei

    Expressed in the developing epidermis and intestinal epithelium. First detected in the epidermis at stage 13.5-14.5 dpc with higher levels in the head and thorax regions. At 15.5 dpc, expression is found in both the epithelium and, to a lesser extent in the underlying mesenchyme. At day 16.5 dpc, high expression in the basal cells. Later expression is found in the developing hair follicles, around the dermal papillae. In the developing intestinal epithelium, expression first observed around 14.5 dpc. Levels continue to increase at least until 19.5 dpc, with highest levels in the intervillus epithelium and in the bottom half of the villi. In the nervous system, first expressed at 9.5 dpc, in the forebrain. At 10.5 dpc, expressed broadly in the brain, and at lower levels in the upper regions of the spinal cord. By 11.5 dpc, E2F2 expression is found throughout the central nervous system and levels peak at 12.5-15.5 dpc. In the developing spinal cord, E2F2 expression found only in the dorsal region. In the developing retina, highest expression found in the 14.5-18.5 dpc embryonic retinoblastic cell layer. In other developing tissues, E2F2 is found highest in thymus and liver, with lower expression in lung, heart, kidney and skeletal muscle. Also found in choroid plexus and chondrocytes.2 Publications

    Gene expression databases

    BgeeiP56931.
    CleanExiMM_E2F2.
    GenevestigatoriP56931.

    Interactioni

    Subunit structurei

    Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP.

    Protein-protein interaction databases

    BioGridi232447. 3 interactions.
    DIPiDIP-48416N.
    IntActiP56931. 1 interaction.
    MINTiMINT-4110723.

    Structurei

    3D structure databases

    ProteinModelPortaliP56931.
    SMRiP56931. Positions 130-194, 208-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 10741Cyclin A/CDK2 bindingSequence AnalysisAdd
    BLAST
    Regioni157 – 17822Leucine-zipperAdd
    BLAST
    Regioni199 – 29193DimerizationSequence AnalysisAdd
    BLAST
    Regioni361 – 44383TransactivationSequence AnalysisAdd
    BLAST
    Regioni416 – 43318Retinoblastoma protein bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi162 – 19837DEF boxAdd
    BLAST

    Sequence similaritiesi

    Belongs to the E2F/DP family.Curated

    Phylogenomic databases

    eggNOGiNOG289227.
    GeneTreeiENSGT00550000074403.
    HOGENOMiHOG000232045.
    HOVERGENiHBG002227.
    InParanoidiA2AW43.
    KOiK09389.
    OMAiKELMSME.
    OrthoDBiEOG738058.
    PhylomeDBiP56931.
    TreeFamiTF105566.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12081. PTHR12081. 1 hit.
    PfamiPF02319. E2F_TDP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56931-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRAPRTLAP ATAQPTKSLP ALNPTELWPS GLSSPQLCPA TTATTYYTSL    50
    YTQTVPSSVA LGTCLDATPH GPEGQIVRCA PAGRLPAKRK LDLEGIGRPT 100
    VPEFRTPKGK CIRVDGLPSP KTPKSPGEKT RYDTSLGLLT KKFIYLLSES 150
    EDGVLDLNWA AEVLDVQKRR IYDITNVLEG IQLIRKKSKN NIQWVGRELF 200
    EDPTRPSRQQ QLGQELKELM NAEQTLDQLI QSCSLSFKHL TEDNANKKLA 250
    YVTYQDIRAV GNFKEQTVIA VKAPPQTRLE VPDRAEENLQ IYLKSTQGPI 300
    EVYLCPEEGQ EPDSPAKEAL PSTSALSPIP DCAQPGCSTD SGIAETIEPS 350
    VLIPQPIPPP PPPPLPPAPS LVPLEATDNM LELSHPLLQQ TEDQFLSPIL 400
    AANSPLISFS PPLDQDEYLW GMDEGEGISD LFDSYDLGDL LIN 443
    Length:443
    Mass (Da):48,499
    Last modified:August 30, 2005 - v2
    Checksum:iDFC8158E5A8254F0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK087452 mRNA. Translation: BAC39881.1.
    AL935264 Genomic DNA. Translation: CAM13832.1.
    BC062101 mRNA. Translation: AAH62101.1.
    CCDSiCCDS18801.1.
    RefSeqiNP_808401.1. NM_177733.6.
    UniGeneiMm.307932.

    Genome annotation databases

    EnsembliENSMUST00000061721; ENSMUSP00000050047; ENSMUSG00000018983.
    GeneIDi242705.
    KEGGimmu:242705.
    UCSCiuc008vht.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK087452 mRNA. Translation: BAC39881.1 .
    AL935264 Genomic DNA. Translation: CAM13832.1 .
    BC062101 mRNA. Translation: AAH62101.1 .
    CCDSi CCDS18801.1.
    RefSeqi NP_808401.1. NM_177733.6.
    UniGenei Mm.307932.

    3D structure databases

    ProteinModelPortali P56931.
    SMRi P56931. Positions 130-194, 208-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 232447. 3 interactions.
    DIPi DIP-48416N.
    IntActi P56931. 1 interaction.
    MINTi MINT-4110723.

    PTM databases

    PhosphoSitei P56931.

    Proteomic databases

    PaxDbi P56931.
    PRIDEi P56931.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000061721 ; ENSMUSP00000050047 ; ENSMUSG00000018983 .
    GeneIDi 242705.
    KEGGi mmu:242705.
    UCSCi uc008vht.2. mouse.

    Organism-specific databases

    CTDi 1870.
    MGIi MGI:1096341. E2f2.

    Phylogenomic databases

    eggNOGi NOG289227.
    GeneTreei ENSGT00550000074403.
    HOGENOMi HOG000232045.
    HOVERGENi HBG002227.
    InParanoidi A2AW43.
    KOi K09389.
    OMAi KELMSME.
    OrthoDBi EOG738058.
    PhylomeDBi P56931.
    TreeFami TF105566.

    Miscellaneous databases

    ChiTaRSi E2f2. mouse.
    NextBioi 385512.
    PROi P56931.
    SOURCEi Search...

    Gene expression databases

    Bgeei P56931.
    CleanExi MM_E2F2.
    Genevestigatori P56931.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12081. PTHR12081. 1 hit.
    Pfami PF02319. E2F_TDP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Expression patterns of the E2F family of transcription factors during murine epithelial development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Cell Growth Differ. 8:553-563(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE OF 49-254, DEVELOPMENTAL STAGE.
      Tissue: Embryo.
    5. "Expression patterns of the E2F family of transcription factors during mouse nervous system development."
      Dagnino L., Fry C.J., Bartley S.M., Farnham P., Gallie B.L., Phillips R.A.
      Mech. Dev. 66:13-25(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    6. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
      Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
      Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EAPP.

    Entry informationi

    Entry nameiE2F2_MOUSE
    AccessioniPrimary (citable) accession number: P56931
    Secondary accession number(s): A2AW43, Q8BID0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3