P56913 (NUOF2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit F 2
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit F 2
NDH-1 subunit F 2

Gene names

Name:	nuoF2
Ordered Locus Names:	RA0829
ORF Names:	SMa1525

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	NADH-quinone oxidoreductase subunit F 2		PRO_0000118577
Regions
	Nucleotide binding	53 – 62	10	NAD By similarity
	Nucleotide binding	165 – 212	48	FMN By similarity
Sites
	Metal binding	342	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	345	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	348	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	388	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	149	1	I → V in CAB51634. Ref.1
	Sequence conflict	155	1	G → K in CAB51634. Ref.1
	Sequence conflict	407	1	A → E in CAB51634. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P56913 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: C0C448920882D7C0	FASTA	421	45,504
	10 20 30 40 50 60 MFEPVLLRNV DVPDGHLLST YEAGGGYRAL RKALGEYTPD EIVELVKESN LRGRGGAGFP 70 80 90 100 110 120 TGMKWSFVPK AAGKPKYLCC NADEGEPGTF KDRIIMERDP HQLIEGLAVS AYAIGAETAY 130 140 150 160 170 180 VYIRGEYVTA IRRMEQAIAE AHENGYLGIG ILGSGFNFMV HIHRGAGAYI CGEETAMLES 190 200 210 220 230 240 LEGKRAQPRL KPPFPAVAGL YASPTVINNV ETLACVPHIV MRGSAWFRGI GPDRSPGPKL 250 260 270 280 290 300 YCLSGQVRKP GLYELPMGIS LRELVEEHAG GPLPGRKVKA VIPGGVSAPV IPEGELEVGM 310 320 330 340 350 360 DFDSLTAAGS MLGSAGVVVI DDSTCMVKLA TRIIEFFHHE SCGKCTPCRE GLDWTVKVLR 370 380 390 400 410 420 RIEAGEGETG DLEQLEMLCK GIFGNTFCAL GDGAAMGLRA ALKHFRAEFV AHIEERRCPF H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhizobium meliloti carries two sets of nuo genes." Putnoky P., Jady B., Chellapilla K.P., Barta F., Kiss E. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[2]	"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ245399 Genomic DNA. Translation: CAB51634.1. AE006469 Genomic DNA. Translation: AAK65487.1.
PIR	E95365.
RefSeq	NP_436075.1. NC_003037.1.
3D structure databases
ProteinModelPortal	P56913.
SMR	P56913. Positions 2-418.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1235865.
GenomeReviews	Gene locus RA0829 in contig AE006469_GR.
KEGG	sme:SMa1525.
NMPDR	fig|266834.1.peg.829.
PATRIC	23628162. VBISinMel96828_0860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG669338.
OMA	WAGGPLE.
ProtClustDB	CLSK807691.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA1525-MONOMER.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
KO	K00335.
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. NuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF2_RHIME

Accession

Primary (citable) accession number: P56913

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 2, 2001
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program