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P56904 (THID_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

EC=2.7.1.49
EC=2.7.4.7
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name=HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name=HMP-P kinase
Short name=HMP-phosphate kinase
Short name=HMPP kinase
Gene names
Name:thiD
Ordered Locus Names:RB1087
ORF Names:SMb20962
Encoded onPlasmid pSymB (megaplasmid 2)
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.

Catalytic activity

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.

ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3.

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.

Sequence similarities

Belongs to the ThiD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
PRO_0000192025

Sites

Binding site431Substrate By similarity

Experimental info

Sequence conflict98 – 992KR → RL in AAD46983. Ref.1
Sequence conflict1151L → F in AAD46983. Ref.1
Sequence conflict1341V → A in AAD46983. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P56904 [UniParc].

Last modified November 2, 2001. Version 2.
Checksum: 803D0C58EB9A1830

FASTA26627,423
        10         20         30         40         50         60 
MTAIALSIAG SDSGGGAGIQ ADLKTFSALG VYGASVITAI TAQNTRGVTA VEDVSAEIVS 

        70         80         90        100        110        120 
AQMDAVFSDL DVKAVKIGMV SRRETIAAIA DGLRRFGKRA VVDPVMVATS GDALLRPDAV 

       130        140        150        160        170        180 
AALIEELLPL ALVVTPNLAE AALMTGRAIA GDEAEMARQA EAIMRTGAHA VLVKGGHLKG 

       190        200        210        220        230        240 
QEATDLFFDG DTLVRLPAGR IETRNDHGTG CTLSAAIAAG LAKGVPLIEA VSAAKAYLHA 

       250        260 
AISAADRLEI GQGRGPVHHF HRWWKD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a DNA fragment related to salt tolerance in Sinorhizobium meliloti."
Yang S., Chen X.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 042B.
[2]"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[4]"Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment."
Becker A., Kleickmann A., Keller M., Arnold W., Puehler A.
Mol. Gen. Genet. 241:367-379(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-266.
Strain: RCR2011 / SU47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF070520 Genomic DNA. Translation: AAD46983.1.
AL591985 Genomic DNA. Translation: CAC49487.1.
Z22636 Genomic DNA. Translation: CAA80350.1. Sequence problems.
PIRG95977.
S78110.
RefSeqNP_437627.1. NC_003078.1.

3D structure databases

ProteinModelPortalP56904.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SM_b20962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC49487; CAC49487; SM_b20962.
GeneID1237418.
KEGGsme:SM_b20962.
PATRIC23638592. VBISinMel96828_6017.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0351.
KOK00941.
OMAFAFHCVH.
OrthoDBEOG6XWV53.
ProtClustDBCLSK801049.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-4520-MONOMER.
UniPathwayUPA00060; UER00137.
UPA00060; UER00138.

Family and domain databases

InterProIPR013749. HMP-P_kinase-1.
IPR004399. HMP-P_kinase-2.
[Graphical view]
PfamPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
TIGRFAMsTIGR00097. HMP-P_kinase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHID_RHIME
AccessionPrimary (citable) accession number: P56904
Secondary accession number(s): Q52914
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: November 13, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways