Reviewed, UniProtKB/Swiss-Prot P56897 (NUOB2_RHIME)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B 2
    NDH-1 subunit B 2

Gene names

Name:	nuoB2
Ordered Locus Names:	RA0833
ORF Names:	SMa1532

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	NADH-quinone oxidoreductase subunit B 2		PRO_0000118776
Sites
	Metal binding	38	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	39	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	103	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	132	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	167	1	S → L in CAB51630. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P56897-1 [UniParc]. Last modified October 24, 2001. Version 2. Checksum: 04D82AAED624D8CF	FASTA	167	18,247
	10 20 30 40 50 60 MAGVNDAIRD SVLFTTADSI ISWSRRSALW PETFGIACCA IEMISAGCAR YDLDRFGVVF 70 80 90 100 110 120 RPSPRQSDVM IIAGTVTRKF APVVRRLYDQ MPEPRWVIAM GTCAISGGVY NTYAVVQGSE 130 140 150 160 TFVPVDVHVP GCPPRPEALM HGFLLLQEKI KKSRALTGTP LGRVIAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhizobium meliloti carries two sets of nuo genes." Putnoky P., Jady B., Chellapilla K.P., Barta F., Kiss E. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[2]	"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245399 Genomic DNA. Translation: CAB51630.1. AE006469 Genomic DNA. Translation: AAK65491.1.
PIR	A95366.
RefSeq	NP_436079.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1235869.
GenomeReviews	Gene locus RA0833 in contig AE006469_GR.
KEGG	sme:SMa1532.
NMPDR	fig|266834.1.peg.833.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P56897.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA1532-MON.
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUOB2_RHIME

Accession

Primary (citable) accession number: P56897

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 24, 2001
Last modified:	November 25, 2008
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)