Reviewed, UniProtKB/Swiss-Prot P56895 (NUOA2_RHIME)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A 2
    NDH-1 subunit A 2

Gene names

Name:	nuoA2
Ordered Locus Names:	RA0834
ORF Names:	SMa1533

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 121	121	NADH-quinone oxidoreductase subunit A 2		PRO_0000117873
Regions
	Transmembrane	6 – 26	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	89 – 109	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P56895-1 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: DBF7B89E856039E8	FASTA	121	13,481
	10 20 30 40 50 60 MTAMEFLPVL FMVTGIVLVA AATLFVSSLL RPSNPYPEKN APYECGMEAA GEAAGGRFRV 70 80 90 100 110 120 PFFILAILLV VFDVEAMFLF PWAVVLKEIG FVGYIEMFVF MLLLLVGFAY AWLKGALEWQ E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhizobium meliloti carries two sets of nuo genes." Putnoky P., Jady B., Chellapilla K.P., Barta F., Kiss E. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[2]	"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245399 Genomic DNA. Translation: CAB51629.1. AE006469 Genomic DNA. Translation: AAK65492.1.
PIR	B95366.
RefSeq	NP_436080.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1235870.
GenomeReviews	Gene locus RA0834 in contig AE006469_GR.
KEGG	sme:SMa1533.
NMPDR	fig|266834.1.peg.834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P56895.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA1533-MON.
Family and domain databases
InterPro	IPR000440. Oxidored_q4. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_RHIME

Accession

Primary (citable) accession number: P56895

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)