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P56888

- ALF_SINMW

UniProt

P56888 - ALF_SINMW

Protein

Fructose-bisphosphate aldolase

Gene

cbbA

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501Glyceraldehyde 3-phosphateBy similarity
    Active sitei83 – 831Proton donorBy similarity
    Metal bindingi84 – 841Zinc 1; catalyticBy similarity
    Metal bindingi105 – 1051Zinc 2By similarity
    Metal bindingi142 – 1421Zinc 2By similarity
    Metal bindingi198 – 1981Zinc 1; catalyticBy similarity
    Binding sitei199 – 1991Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi232 – 2321Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Calvin cycle, Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSMED366394:GJAL-3984-MONOMER.
    UniPathwayiUPA00109; UER00183.
    UPA00116.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:cbbA
    Ordered Locus Names:Smed_3923
    Encoded oniPlasmid pSMED010 Publication
    OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
    Taxonomic identifieri366394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001108: Plasmid pSMED01

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Fructose-bisphosphate aldolasePRO_0000178730Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi366394.Smed_3923.

    Structurei

    3D structure databases

    ProteinModelPortaliP56888.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni233 – 2353Dihydroxyacetone phosphate bindingBy similarity
    Regioni275 – 2784Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0191.
    HOGENOMiHOG000227792.
    KOiK01624.
    OMAiERYLANM.
    OrthoDBiEOG6HXJ7B.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006412. Fruct_bisP_Calv.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01521. FruBisAldo_II_B. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56888-1 [UniParc]FASTAAdd to Basket

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    MARITLRQLL DHAAERSYGV PAFNINNMEQ GLAIMEAARA CDAPVILQVS    50
    RGARSYANDI MLAKMMEALE VMYPDIPLCI HQDHGNNVAT CLTAIQHGFT 100
    SVMMDGSLKE DAKTPADYAY NAMITTEVSR LAHMVGASVE GELGCLGSLE 150
    TGHGEAEDGH GFEGALDRSQ LLTDPDEAAR FVAETGVDAL AVAIGTSHGA 200
    YKFTRKPTGE VLAMDVIEKI HERLPDTHIV MHGSSSVPQE WQDVFNAHGG 250
    EMRETYGVPV EEIVRGIRFG VRKVNIDTDL RLAAAAAFRR VADTSRTEFD 300
    PRKFLKPAMD AMSAVCKARF EAFGTAGNAS RIKVVPMAEM ARRYASGSLK 350
    PQAKRAEAA 359
    Length:359
    Mass (Da):38,925
    Last modified:November 13, 2007 - v2
    Checksum:i00D2342753333700
    GO

    Sequence cautioni

    The sequence AAF25378.1 differs from that shown. Reason: Frameshift at positions 100 and 120.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 902AT → PN in AAF25378. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF211846 Genomic DNA. Translation: AAF25378.1. Frameshift.
    CP000739 Genomic DNA. Translation: ABR62733.1.
    RefSeqiYP_001312666.1. NC_009620.1.

    Genome annotation databases

    EnsemblBacteriaiABR62733; ABR62733; Smed_3923.
    GeneIDi5318708.
    KEGGismd:Smed_3923.
    PATRICi23613392. VBISinMed134228_0369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF211846 Genomic DNA. Translation: AAF25378.1 . Frameshift.
    CP000739 Genomic DNA. Translation: ABR62733.1 .
    RefSeqi YP_001312666.1. NC_009620.1.

    3D structure databases

    ProteinModelPortali P56888.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 366394.Smed_3923.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR62733 ; ABR62733 ; Smed_3923 .
    GeneIDi 5318708.
    KEGGi smd:Smed_3923.
    PATRICi 23613392. VBISinMed134228_0369.

    Phylogenomic databases

    eggNOGi COG0191.
    HOGENOMi HOG000227792.
    KOi K01624.
    OMAi ERYLANM.
    OrthoDBi EOG6HXJ7B.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    UPA00116 .
    BioCyci SMED366394:GJAL-3984-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006412. Fruct_bisP_Calv.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01521. FruBisAldo_II_B. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic regulation of C1 metabolism in Sinorhizobium meliloti."
      Fenner B.J., Tiwari R.P., Dilworth M.J.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequence of Sinorhizobium medicae WSM419 plasmid pSMED01."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WSM419.

    Entry informationi

    Entry nameiALF_SINMW
    AccessioniPrimary (citable) accession number: P56888
    Secondary accession number(s): A6UGF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3