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P56888

- ALF_SINMW

UniProt

P56888 - ALF_SINMW

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Protein
Fructose-bisphosphate aldolase
Gene
cbbA, Smed_3923
Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Glyceraldehyde 3-phosphate By similarity
Active sitei83 – 831Proton donor By similarity
Metal bindingi84 – 841Zinc 1; catalytic By similarity
Metal bindingi105 – 1051Zinc 2 By similarity
Metal bindingi142 – 1421Zinc 2 By similarity
Metal bindingi198 – 1981Zinc 1; catalytic By similarity
Binding sitei199 – 1991Dihydroxyacetone phosphate; via amide nitrogen By similarity
Metal bindingi232 – 2321Zinc 1; catalytic By similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
  2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Calvin cycle, Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSMED366394:GJAL-3984-MONOMER.
UniPathwayiUPA00109; UER00183.
UPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:cbbA
Ordered Locus Names:Smed_3923
Encoded oniPlasmid pSMED010 Publication
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001108: Plasmid pSMED01

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Fructose-bisphosphate aldolase
PRO_0000178730Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi366394.Smed_3923.

Structurei

3D structure databases

ProteinModelPortaliP56888.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni233 – 2353Dihydroxyacetone phosphate binding By similarity
Regioni275 – 2784Dihydroxyacetone phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227792.
KOiK01624.
OMAiERYLANM.
OrthoDBiEOG6HXJ7B.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006412. Fruct_bisP_Calv.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01521. FruBisAldo_II_B. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56888-1 [UniParc]FASTAAdd to Basket

« Hide

MARITLRQLL DHAAERSYGV PAFNINNMEQ GLAIMEAARA CDAPVILQVS    50
RGARSYANDI MLAKMMEALE VMYPDIPLCI HQDHGNNVAT CLTAIQHGFT 100
SVMMDGSLKE DAKTPADYAY NAMITTEVSR LAHMVGASVE GELGCLGSLE 150
TGHGEAEDGH GFEGALDRSQ LLTDPDEAAR FVAETGVDAL AVAIGTSHGA 200
YKFTRKPTGE VLAMDVIEKI HERLPDTHIV MHGSSSVPQE WQDVFNAHGG 250
EMRETYGVPV EEIVRGIRFG VRKVNIDTDL RLAAAAAFRR VADTSRTEFD 300
PRKFLKPAMD AMSAVCKARF EAFGTAGNAS RIKVVPMAEM ARRYASGSLK 350
PQAKRAEAA 359
Length:359
Mass (Da):38,925
Last modified:November 13, 2007 - v2
Checksum:i00D2342753333700
GO

Sequence cautioni

The sequence AAF25378.1 differs from that shown. Reason: Frameshift at positions 100 and 120.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 902AT → PN in AAF25378. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF211846 Genomic DNA. Translation: AAF25378.1. Frameshift.
CP000739 Genomic DNA. Translation: ABR62733.1.
RefSeqiYP_001312666.1. NC_009620.1.

Genome annotation databases

EnsemblBacteriaiABR62733; ABR62733; Smed_3923.
GeneIDi5318708.
KEGGismd:Smed_3923.
PATRICi23613392. VBISinMed134228_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF211846 Genomic DNA. Translation: AAF25378.1 . Frameshift.
CP000739 Genomic DNA. Translation: ABR62733.1 .
RefSeqi YP_001312666.1. NC_009620.1.

3D structure databases

ProteinModelPortali P56888.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 366394.Smed_3923.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR62733 ; ABR62733 ; Smed_3923 .
GeneIDi 5318708.
KEGGi smd:Smed_3923.
PATRICi 23613392. VBISinMed134228_0369.

Phylogenomic databases

eggNOGi COG0191.
HOGENOMi HOG000227792.
KOi K01624.
OMAi ERYLANM.
OrthoDBi EOG6HXJ7B.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .
UPA00116 .
BioCyci SMED366394:GJAL-3984-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006412. Fruct_bisP_Calv.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01521. FruBisAldo_II_B. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic regulation of C1 metabolism in Sinorhizobium meliloti."
    Fenner B.J., Tiwari R.P., Dilworth M.J.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of Sinorhizobium medicae WSM419 plasmid pSMED01."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM419.

Entry informationi

Entry nameiALF_SINMW
AccessioniPrimary (citable) accession number: P56888
Secondary accession number(s): A6UGF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 13, 2007
Last modified: June 11, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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