P56886 (F16PA_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-1,6-bisphosphatase class 1
Short name=FBPase class 1
EC=3.1.3.11

Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1

Gene names

Name:	fbp
Synonyms:	cbbF
Ordered Locus Names:	Smed_3920

Encoded on

Plasmid pSMED01

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Fructose-1,6-bisphosphatase class 1 HAMAP MF_01855		PRO_0000200485
Regions
	Region	113 – 116	4	Substrate binding By similarity
Sites
	Metal binding	91	1	Magnesium 1 By similarity
	Metal binding	110	1	Magnesium 1 By similarity
	Metal binding	110	1	Magnesium 2 By similarity
	Metal binding	112	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	113	1	Magnesium 2 By similarity
	Metal binding	277	1	Magnesium 2 By similarity
	Binding site	205	1	Substrate By similarity
Experimental info
	Sequence conflict	143	1	Q → H in AAF25375. Ref.1
	Sequence conflict	175	1	E → D in AAF25375. Ref.1
	Sequence conflict	322	1	A → G in AAF25375. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P56886 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 7031BBED2E887366	FASTA	349	37,999
	10 20 30 40 50 60 MSGATLEAYL ASCTTHGDEL SRDVAAVIQR LAKAALDIRK LVNQGALGTV FNGMHSGSNT 70 80 90 100 110 120 DGDVQKDLDI LCDDQFLSCL QGAPVACYAS EELENPVLLD PAARLAVAID PLDGSSNIDN 130 140 150 160 170 180 NISIGTIFSV LPAAKGPDVD PSQSFLQPGN RQLAAGFFVY GPQTALVLSL GRGTEIFIFS 190 200 210 220 230 240 SRLGCFVDAY KSVGIPDRAN EFAINMSNYR HWEEAIRLYV DDCLAGSEGP RERDFNMRWI 250 260 270 280 290 300 ASLVAEAYRI LVRGGIFLYP ADSRKGYSHG RIRLVYEANP IAFIVENAGG SATTSVDRIL 310 320 330 340 DLVPESLHQR VPLVFGSRRE VARITRYHVD PNMIGERAPL FGKRGLFRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic regulation of C1 metabolism in Sinorhizobium meliloti." Fenner B.J., Tiwari R.P., Dilworth M.J. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Sinorhizobium medicae WSM419 plasmid pSMED01." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF211846 Genomic DNA. Translation: AAF25375.1. CP000739 Genomic DNA. Translation: ABR62730.1.
RefSeq	YP_001312663.1. NC_009620.1.
3D structure databases
ProteinModelPortal	P56886.
ModBase	Search...
Protein-protein interaction databases
STRING	P56886.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5318705.
GenomeReviews	Gene locus Smed_3920 in contig CP000739_GR.
KEGG	smd:Smed_3920.
PATRIC	23613386. VBISinMed134228_0366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0158.
HOGENOM	HBG731261.
OMA	HWEAPVQ.
ProtClustDB	PRK09293.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_3920-MONOMER.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. FBPase_class-1/SBPase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
KO	K03841.
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PIRSF	PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16PA_SINMW

Accession

Primary (citable) accession number: P56886
Secondary accession number(s): A6UGF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program