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P56884

- GLND_RHIME

UniProt

P56884 - GLND_RHIME

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:R00396
ORF Names:SMc01124
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192760Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc01124.

Structurei

3D structure databases

ProteinModelPortaliP56884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini494 – 608115HDUniRule annotationAdd
BLAST
Domaini734 – 81683ACT 1UniRule annotationAdd
BLAST
Domaini845 – 92682ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 377377UridylyltransferaseAdd
BLAST
Regioni378 – 733356Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56884-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARHETSFPE ILDVAALRAR CDFIASAHAE QREPMRRALL AAFKEANVAG
60 70 80 90 100
RAKARELLAA DGAGIKCAER ISWLQDQLIT LLHDFVLNQV FDAAKAPEAS
110 120 130 140 150
RIAVTAVGGY GRGTLAPGSD IDLLFLLPAK KAVWAEPAIE FMLYILWDLG
160 170 180 190 200
FKVGHATRTI EECIRLSRAD MTIRTAILEC RYVCGSAALA NELETRFDHE
210 220 230 240 250
IVRNTGPEFI AAKLAERDER HRKAGDTRYL VEPNVKEGKG GLRDLHTLFW
260 270 280 290 300
ISKYFYRVKD SADLVKLGVL SKQEYKLFQK AEDFLWAVRC HMHFLTGKAE
310 320 330 340 350
ERLSFDIQRE IAEALGYHDH PGLSAVERFM KHYFLVAKDV GDLTRIFCSA
360 370 380 390 400
LEDQQAKDTP GITGVISRFR NRVRKIAGTL DFVDDGGRIA LAGTDVFKRD
410 420 430 440 450
PVNLMRLFHI ADINGLEFHP AALKQVTRSL GLITPHLREN EEANRLFLSI
460 470 480 490 500
LTSRRNPELI LRRMNEAGVL GRFIPEFGKI VSMMQFNMYH HYTVDEHLLR
510 520 530 540 550
SVDVLSRIER GLEEEAHPLT AMLMPGIEDR EALYVAVLLH DIAKGRPEDH
560 570 580 590 600
SVAGAKVARK LCPRFRLTPK QTEMVVWLVE EHLTMSMVAQ TRDLNDRKTI
610 620 630 640 650
VDFAERVQSL ERLKMLLILT VCDIRAVGPG VWNGWKGQLL RTLYYETELL
660 670 680 690 700
LSGGFSELSR KERAKHAAHM LEEALADWPK KERQAYVRLH YQPYLLTVAL
710 720 730 740 750
EEQVRHAGFI READRAGRTL ATMVRTHDFH AITEITVLSP DHPRLLTVIA
760 770 780 790 800
GACAAAGANI VGAQIHTTSD GRALDTILVN REFSVAEDET RRAASIGKLI
810 820 830 840 850
EDVLSGRKRL PEVIASRTRV KKRSRAFTVT PEVTISNTLS NKFTVIEVEG
860 870 880 890 900
LDRTGLLSEV TAVLSDLSLD IASAHITTFG EKVIDTFYVT DLVGSKITSE
910 920 930 940
NRQMNIAARL KAVLAGEVDE ARERMPSGII APTPVSRVPH GSKTTKAET
Length:949
Mass (Da):106,382
Last modified:May 30, 2000 - v1
Checksum:iEF549C1B0D8540A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF227730 Genomic DNA. Translation: AAF37852.1.
AL591688 Genomic DNA. Translation: CAC41833.1.
RefSeqiNP_384502.1. NC_003047.1.
WP_003527696.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC41833; CAC41833; SMc01124.
GeneIDi1232030.
KEGGisme:SMc01124.
PATRICi23629999. VBISinMel96828_1769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF227730 Genomic DNA. Translation: AAF37852.1 .
AL591688 Genomic DNA. Translation: CAC41833.1 .
RefSeqi NP_384502.1. NC_003047.1.
WP_003527696.1. NC_003047.1.

3D structure databases

ProteinModelPortali P56884.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266834.SMc01124.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC41833 ; CAC41833 ; SMc01124 .
GeneIDi 1232030.
KEGGi sme:SMc01124.
PATRICi 23629999. VBISinMel96828_1769.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci SMEL266834:GJF6-407-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "glnD and mviN are genes of an essential operon in Sinorhizobium meliloti."
    Rudnick P.A., Arcondeguy T., Kennedy C.K., Kahn D.
    J. Bacteriol. 183:2682-2685(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiGLND_RHIME
AccessioniPrimary (citable) accession number: P56884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3