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Protein

Alkyl hydroperoxide reductase C

Gene

ahpC

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.By similarity

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide (PubMed:16213196). The disulfide is subsequently reduced by thioredoxin (PubMed:11222594).2 Publications

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Cysteine sulfenic acid (-SOH) intermediate1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyl hydroperoxide reductase C (EC:1.11.1.15)
Alternative name(s):
26 kDa antigen
Peroxiredoxin
Thioredoxin peroxidase
Gene namesi
Name:ahpC
Synonyms:tsaA
Ordered Locus Names:jhp_1471
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000804 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001351451 – 198Alkyl hydroperoxide reductase CAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49Interchain (with C-169)Combined sources1 Publication
Disulfide bondi169Interchain (with C-49)Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP56876.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (PubMed:16213196).1 Publication

Protein-protein interaction databases

IntActiP56876. 1 interactor.
STRINGi85963.jhp1471.

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 15Combined sources5
Beta strandi21 – 26Combined sources6
Turni27 – 29Combined sources3
Beta strandi33 – 40Combined sources8
Helixi52 – 58Combined sources7
Helixi60 – 65Combined sources6
Beta strandi69 – 76Combined sources8
Helixi78 – 85Combined sources8
Helixi89 – 91Combined sources3
Beta strandi101 – 103Combined sources3
Helixi108 – 112Combined sources5
Turni118 – 120Combined sources3
Beta strandi124 – 130Combined sources7
Turni131 – 133Combined sources3
Beta strandi134 – 144Combined sources11
Helixi148 – 163Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZOFX-ray2.95A/B/C/D/E/F/G/H/I/J1-198[»]
ProteinModelPortaliP56876.
SMRiP56876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56876.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 161ThioredoxinPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105D3R. Bacteria.
COG0450. LUCA.
KOiK03386.
OMAiTAVHNGE.
OrthoDBiPOG091H026G.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P56876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVTKLAPDF KAPAVLGNNE VDEHFELSKN LGKNGVILFF WPKDFTFVCP
60 70 80 90 100
TEIIAFDKRV KDFHEKGFNV IGVSIDSEQV HFAWKNTPVE KGGIGQVSFP
110 120 130 140 150
MVADITKSIS RDYDVLFEEA IALRGAFLID KNMKVRHAVI NDLPLGRNAD
160 170 180 190
EMLRMVDALL HFEEHGEVCP AGWRKGDKGM KATHQGVAEY LKENSIKL
Length:198
Mass (Da):22,259
Last modified:May 30, 2000 - v1
Checksum:i3E6C18E6F06D52F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA. Translation: AAD07055.1.
PIRiH71801.
RefSeqiWP_000961648.1. NZ_CP011330.1.

Genome annotation databases

EnsemblBacteriaiAAD07055; AAD07055; jhp_1471.
KEGGihpj:jhp_1471.
PATRICifig|85963.30.peg.1071.

Similar proteinsi

Entry informationi

Entry nameiAHPC_HELPJ
AccessioniPrimary (citable) accession number: P56876
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: September 27, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families