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Protein

Circulin-A

Gene
N/A
Organism
Chassalia parviflora
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism. Has antibiotic activity. Inhibits the cytopathic effects and replication of the human immunodeficiency virus. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Circulin-A
Short name:
CIRA
OrganismiChassalia parviflora
Taxonomic identifieri58431 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeRubioideaePalicoureeaeChassalia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3030Circulin-APRO_0000043599Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1 ↔ 30Cyclopeptide (Gly-Asn)
Disulfide bondi4 ↔ 20PROSITE-ProRule annotation1 Publication
Disulfide bondi8 ↔ 22PROSITE-ProRule annotation1 Publication
Disulfide bondi13 ↔ 27PROSITE-ProRule annotation1 Publication

Post-translational modificationi

This is a cyclic peptide.

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
30
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Helixi15 – 184Combined sources
Beta strandi28 – 303Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BH4NMR-A4-30[»]
ProteinModelPortaliP56871.
SMRiP56871. Positions 4-30.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56871.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the cyclotide family. Bracelet subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
PIRSFiPIRSF037891. Cycloviolacin. 1 hit.
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30
GIPCGESCVW IPCISAALGC SCKNKVCYRN
Length:30
Mass (Da):3,176
Last modified:December 5, 2001 - v2
Checksum:i19D19A52EE9C58CF
GO

Mass spectrometryi

Molecular mass is 3153.3 Da from positions 1 - 30. Determined by FAB. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BH4NMR-A4-30[»]
ProteinModelPortaliP56871.
SMRiP56871. Positions 4-30.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP56871.

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
PIRSFiPIRSF037891. Cycloviolacin. 1 hit.
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIRA_CHAPA
AccessioniPrimary (citable) accession number: P56871
Secondary accession number(s): P82250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 5, 2001
Last modified: December 9, 2015
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

This peptide is cyclic. The start position was chosen by similarity to OAK1 (kalata-B1) for which the DNA sequence is known.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.