Reviewed, UniProtKB/Swiss-Prot P56860 (CYSH_DEIRA)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	DR_A0015

Organism

Deinococcus radiodurans [Complete proteome] [HAMAP]

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100629

Sequences

	Sequence		Length	Mass (Da)
	P56860-1 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: 9A92D09AF13FEA15	FASTA	255	27,751
	10 20 30 40 50 60 MMTAEVRTPE QGGGPLTTEP RAPRSAPGHA DASAPAFGPE TDPRDIIRWA LAAHPDLLMP 70 80 90 100 110 120 SAFNLNGVVL LDLAAQAGYR GEVVFVDTGY HFPETLATRD RLESRYPELT FVTLNAGASP 130 140 150 160 170 180 DDGQTPPDLY ASDPDACCAA RKVDPLQRYL KEQGPSALLN ARSRDQASTR ADIPFVEEGG 190 200 210 220 230 240 ARRRVNPLAH WTREQLEAYA AEHDLPVNPL YFDGFLSIGC WPCTRAVKPG EDARAGRWAG 250 KGKTECGLWQ GENKL

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / IFO 15346 / LMG 4051 / NCIB 9279 / R1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001825 Genomic DNA. Translation: AAF12285.1.
PIR	C75594.
RefSeq	NP_285339.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1798156.
GenomeReviews	Gene locus DR_A0015 in contig AE001825_GR.
KEGG	dra:DR_A0015.
NMPDR	fig|243230.1.peg.2829.
TIGR	DR_A0015.
Phylogenomic databases
HOGENOM	P56860.
OMA	P56860. FEETLAY.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_A0015-MON.
BRENDA	1.8.4.8. 96172.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_DEIRA

Accession

Primary (citable) accession number: P56860

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)