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Protein

Claudin-18

Gene

Cldn18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.By similarity

GO - Molecular functioni

GO - Biological processi

  • calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
  • digestive tract development Source: MGI
  • negative regulation of bone resorption Source: MGI
  • negative regulation of osteoclast development Source: MGI
  • negative regulation of protein localization to nucleus Source: MGI
  • TNFSF11-mediated signaling pathway Source: MGI
Complete GO annotation...

Protein family/group databases

TCDBi1.H.1.1.8. the claudin tight junction (claudin1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-18
Gene namesi
Name:Cldn18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1929209. Cldn18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721HelicalSequence analysisAdd
BLAST
Topological domaini28 – 8053ExtracellularSequence analysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Topological domaini102 – 12221CytoplasmicSequence analysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence analysisAdd
BLAST
Topological domaini144 – 17633ExtracellularSequence analysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence analysisAdd
BLAST
Topological domaini198 – 26467CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Claudin-18PRO_0000144780Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56857.
PaxDbiP56857.
PRIDEiP56857.

PTM databases

iPTMnetiP56857.
PhosphoSiteiP56857.

Expressioni

Gene expression databases

BgeeiP56857.
CleanExiMM_CLDN18.
GenevisibleiP56857. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48956N.
IntActiP56857. 1 interaction.
MINTiMINT-4090972.
STRINGi10090.ENSMUSP00000035048.

Structurei

3D structure databases

ProteinModelPortaliP56857.
SMRiP56857. Positions 7-195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG28. Eukaryota.
ENOG410XPD1. LUCA.
GeneTreeiENSGT00760000119222.
HOGENOMiHOG000220937.
HOVERGENiHBG102991.
InParanoidiP56857.
KOiK06087.
OMAiNRYTFGS.
OrthoDBiEOG76HQ2T.
PhylomeDBiP56857.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003928. Claudin18.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 2 hits.
PTHR12002:SF9. PTHR12002:SF9. 2 hits.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01448. CLAUDIN18.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A1.1 (identifier: P56857-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTTCQVVG LLLSLLGLAG CIAATGMDMW STQDLYDNPV TAVFQYEGLW
60 70 80 90 100
RSCVQQSSGF TECRPYFTIL GLPAMLQAVR ALMIVGIVLG VIGILVSIFA
110 120 130 140 150
LKCIRIGSMD DSAKAKMTLT SGILFIISGI CAIIGVSVFA NMLVTNFWMS
160 170 180 190 200
TANMYSGMGG MGGMVQTVQT RYTFGAALFV GWVAGGLTLI GGVMMCIACR
210 220 230 240 250
GLTPDDSNFK AVSYHASGQN VAYRPGGFKA STGFGSNTRN KKIYDGGART
260
EDDEQSHPTK YDYV
Length:264
Mass (Da):28,122
Last modified:May 30, 2000 - v1
Checksum:i3CA0D441C4705653
GO
Isoform A1.2 (identifier: P56857-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     208-208: N → K
     209-264: Missing.

Show »
Length:208
Mass (Da):21,978
Checksum:iFA9E7DA70D7E7ADE
GO
Isoform A2.1 (identifier: P56857-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MATTTCQVVG...FTECRPYFTI → MSVTACQGLG...FTECRGYFTL

Show »
Length:264
Mass (Da):28,144
Checksum:iAEA19430E157E831
GO
Isoform A2.2 (identifier: P56857-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MATTTCQVVG...FTECRPYFTI → MSVTACQGLG...FTECRGYFTL
     208-208: N → K
     209-264: Missing.

Show »
Length:208
Mass (Da):22,000
Checksum:i03FAA247911BCD6C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6969MATTT…PYFTI → MSVTACQGLGFVVSLIGFAG IIAATCMDQWSTQDLYNNPV TAVFNYQGLWRSCVRESSGF TECRGYFTL in isoform A2.1 and isoform A2.2. CuratedVSP_001103Add
BLAST
Alternative sequencei208 – 2081N → K in isoform A1.2 and isoform A2.2. CuratedVSP_001104
Alternative sequencei209 – 26456Missing in isoform A1.2 and isoform A2.2. CuratedVSP_001105Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF221068 mRNA. Translation: AAF26447.1.
AF349450 mRNA. Translation: AAL15635.1.
AF349451 mRNA. Translation: AAL15636.1.
AF349453 mRNA. Translation: AAL15638.1.
CCDSiCCDS23437.1. [P56857-1]
CCDS57692.1. [P56857-2]
CCDS57693.1. [P56857-4]
CCDS57694.1. [P56857-3]
RefSeqiNP_001181850.1. NM_001194921.1. [P56857-3]
NP_001181851.1. NM_001194922.1. [P56857-2]
NP_001181852.1. NM_001194923.1. [P56857-4]
NP_062789.1. NM_019815.3. [P56857-1]
UniGeneiMm.386784.

Genome annotation databases

EnsembliENSMUST00000035048; ENSMUSP00000035048; ENSMUSG00000032473. [P56857-1]
ENSMUST00000112882; ENSMUSP00000108503; ENSMUSG00000032473. [P56857-3]
ENSMUST00000131922; ENSMUSP00000117382; ENSMUSG00000032473. [P56857-4]
ENSMUST00000136429; ENSMUSP00000115782; ENSMUSG00000032473. [P56857-2]
GeneIDi56492.
KEGGimmu:56492.
UCSCiuc009rep.2. mouse. [P56857-1]
uc009req.2. mouse. [P56857-2]
uc009rer.2. mouse. [P56857-4]
uc009res.2. mouse. [P56857-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF221068 mRNA. Translation: AAF26447.1.
AF349450 mRNA. Translation: AAL15635.1.
AF349451 mRNA. Translation: AAL15636.1.
AF349453 mRNA. Translation: AAL15638.1.
CCDSiCCDS23437.1. [P56857-1]
CCDS57692.1. [P56857-2]
CCDS57693.1. [P56857-4]
CCDS57694.1. [P56857-3]
RefSeqiNP_001181850.1. NM_001194921.1. [P56857-3]
NP_001181851.1. NM_001194922.1. [P56857-2]
NP_001181852.1. NM_001194923.1. [P56857-4]
NP_062789.1. NM_019815.3. [P56857-1]
UniGeneiMm.386784.

3D structure databases

ProteinModelPortaliP56857.
SMRiP56857. Positions 7-195.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48956N.
IntActiP56857. 1 interaction.
MINTiMINT-4090972.
STRINGi10090.ENSMUSP00000035048.

Protein family/group databases

TCDBi1.H.1.1.8. the claudin tight junction (claudin1) family.

PTM databases

iPTMnetiP56857.
PhosphoSiteiP56857.

Proteomic databases

MaxQBiP56857.
PaxDbiP56857.
PRIDEiP56857.

Protocols and materials databases

DNASUi56492.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035048; ENSMUSP00000035048; ENSMUSG00000032473. [P56857-1]
ENSMUST00000112882; ENSMUSP00000108503; ENSMUSG00000032473. [P56857-3]
ENSMUST00000131922; ENSMUSP00000117382; ENSMUSG00000032473. [P56857-4]
ENSMUST00000136429; ENSMUSP00000115782; ENSMUSG00000032473. [P56857-2]
GeneIDi56492.
KEGGimmu:56492.
UCSCiuc009rep.2. mouse. [P56857-1]
uc009req.2. mouse. [P56857-2]
uc009rer.2. mouse. [P56857-4]
uc009res.2. mouse. [P56857-3]

Organism-specific databases

CTDi51208.
MGIiMGI:1929209. Cldn18.

Phylogenomic databases

eggNOGiENOG410IG28. Eukaryota.
ENOG410XPD1. LUCA.
GeneTreeiENSGT00760000119222.
HOGENOMiHOG000220937.
HOVERGENiHBG102991.
InParanoidiP56857.
KOiK06087.
OMAiNRYTFGS.
OrthoDBiEOG76HQ2T.
PhylomeDBiP56857.
TreeFamiTF331936.

Miscellaneous databases

PROiP56857.
SOURCEiSearch...

Gene expression databases

BgeeiP56857.
CleanExiMM_CLDN18.
GenevisibleiP56857. MM.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003928. Claudin18.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 2 hits.
PTHR12002:SF9. PTHR12002:SF9. 2 hits.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01448. CLAUDIN18.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and stomach-specific isoforms through alternative splicing."
    Niimi T., Nagashima K., Ward J.M., Minoo P., Zimonjic D.B., Popescu N.C., Kimura S.
    Mol. Cell. Biol. 21:7380-7390(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.

Entry informationi

Entry nameiCLD18_MOUSE
AccessioniPrimary (citable) accession number: P56857
Secondary accession number(s): Q91ZY9, Q91ZZ0, Q91ZZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: June 8, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.