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Protein

Kappa-theraphotoxin-Scg1a

Gene
N/A
Organism
Stromatopelma calceatum griseipes (Feather leg baboon tarantula) (Scodra griseipes)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly inhibits potassium currents in oocytes expressing Kv2.1/KCNB1 channels. Acts by shifting activation of the channel to more depolarized voltages. The toxin may bind to the S3b-S4 helices of the voltage sensor paddle. One, two, three or four toxin molecules may bind the Kv2.1/KCNB1 channel. Partitions into the bilayer membrane, where it stabilizes at the water/membrane interface.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei3May be involved in interaction with voltage sensor1 Publication1
Sitei5May be involved in interaction with voltage sensor1 Publication1
Sitei6May be involved in interaction with voltage sensor1 Publication1
Sitei22May be involved in interaction with voltage sensor1 Publication1
Sitei30May be involved in interaction with voltage sensor, positioned at 9 angstroms from the center of the bilayer1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Scg1a
Short name:
Kappa-TRTX-Scg1a
Alternative name(s):
Toxin SGTx11 Publication
OrganismiStromatopelma calceatum griseipes (Feather leg baboon tarantula) (Scodra griseipes)
Taxonomic identifieri118974 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeStromatopelma

Organism-specific databases

ArachnoServeriAS000227. kappa-theraphotoxin-Scg1a.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: UniProtKB
  • other organism cell membrane Source: UniProtKB

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1T → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi3R → A: Exhibits <150-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi4Y → A: Exhibits 7.5-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi5L → A: Exhibits <150-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi6F → A: Exhibits <300-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi7G → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi8G → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi10K → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi11T → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi12T → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi13A → S: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi14D → A: Exhibits 6-fold tigher interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi17K → A: No change in interaction with Kv2.1. 1 Publication1
Mutagenesisi18H → A: Exhibits 7.5-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi20A → S: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi22R → A: Exhibits <150-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi23S → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi24D → A: Exhibits 20-fold tigher interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi25G → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi26K → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi29A → S: Exhibits 7.5-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi30W → A: Exhibits <300-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi31D → A: Exhibits 11.3-fold weaker interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi32G → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi33T → A: No change in interaction with Kv2.1 channels. 1 Publication1
Mutagenesisi34F → A: No change in interaction with Kv2.1 channels. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000450241 – 34Kappa-theraphotoxin-Scg1aAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi2 ↔ 161 Publication
Disulfide bondi9 ↔ 211 Publication
Disulfide bondi15 ↔ 281 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Protein-protein interaction databases

IntActiP56855. 1 interactor.

Structurei

Secondary structure

134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 14Combined sources3
Turni23 – 26Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LA4NMR-A1-34[»]
ProteinModelPortaliP56855.
SMRiP56855.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56855.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 6Involved in active face1 Publication3

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.1 Publication

Sequence similaritiesi

Belongs to the huwentoxin-1 family. HaTx subfamily.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.

Sequencei

Sequence statusi: Complete.

P56855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
TCRYLFGGCK TTADCCKHLA CRSDGKYCAW DGTF
Length:34
Mass (Da):3,782
Last modified:May 30, 2000 - v1
Checksum:i31B44D44BAF814A4
GO

Mass spectrometryi

Molecular mass is 3775.7±0.6 Da from positions 1 - 34. Determined by MALDI. 1 Publication
Molecular mass is 3776.7 Da from positions 1 - 34. Determined by MALDI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LA4NMR-A1-34[»]
ProteinModelPortaliP56855.
SMRiP56855.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56855. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000227. kappa-theraphotoxin-Scg1a.

Miscellaneous databases

EvolutionaryTraceiP56855.

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTX1_STRGF
AccessioniPrimary (citable) accession number: P56855
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.