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P56853

- TXHN2_GRARO

UniProt

P56853 - TXHN2_GRARO

Protein

Kappa-theraphotoxin-Gr1b

Gene
N/A
Organism
Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibitor of voltage-gated potassium channels. Inhibits Kv2.1/KCNB1 channels, by shifting activation of the channel to more depolarized voltages. The toxin binding sites may be situated on the S3-S4 extracellular linker of the channel. One, two, three or four toxin molecules may bind the Kv2.1/KCNB1 channel. May need to partition into the membrane in order to bind to the channel. Antibacterial activity is not observed.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei3 – 31May be involved in interaction with voltage sensorBy similarity
    Sitei22 – 221May be involved in interaction with voltage sensorBy similarity
    Sitei30 – 301Involved in active faceBy similarity

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kappa-theraphotoxin-Gr1b
    Short name:
    Kappa-TRTX-Gr1b
    Alternative name(s):
    Hanatoxin-2
    Short name:
    HaTx2
    OrganismiGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
    Taxonomic identifieri432528 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

    Organism-specific databases

    ArachnoServeriAS000343. kappa-theraphotoxin-Gr1b.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 3535Kappa-theraphotoxin-Gr1bPRO_0000045013Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi2 ↔ 16By similarity
    Disulfide bondi9 ↔ 21By similarity
    Disulfide bondi15 ↔ 28By similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    ProteinModelPortaliP56853.
    SMRiP56853. Positions 1-35.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 63Involved in active faceBy similarity

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

    Sequence similaritiesi

    Belongs to the huwentoxin-1 family.Curated

    Keywords - Domaini

    Knottin

    Family and domain databases

    InterProiIPR011696. Ion_channel_inhibitory.
    [Graphical view]
    PfamiPF07740. Toxin_12. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56853-1 [UniParc]FASTAAdd to Basket

    « Hide

    ECRYLFGGCK TTADCCKHLG CKFRDKYCAW DFTFS                   35
    Length:35
    Mass (Da):4,105
    Last modified:May 30, 2000 - v1
    Checksum:i5601AC8A9B14C513
    GO

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P56853.
    SMRi P56853. Positions 1-35.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ArachnoServeri AS000343. kappa-theraphotoxin-Gr1b.

    Family and domain databases

    InterProi IPR011696. Ion_channel_inhibitory.
    [Graphical view ]
    Pfami PF07740. Toxin_12. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantula."
      Swartz K.J., MacKinnon R.
      Neuron 15:941-949(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Venom.

    Entry informationi

    Entry nameiTXHN2_GRARO
    AccessioniPrimary (citable) accession number: P56853
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3