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P56853 (TXHN2_GRARO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kappa-theraphotoxin-Gr1b

Short name=Kappa-TRTX-Gr1b
Alternative name(s):
Hanatoxin-2
Short name=HaTx2
OrganismGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Taxonomic identifier432528 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

Protein attributes

Sequence length35 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of voltage-gated potassium channels. Inhibits Kv2.1/KCNB1 channels, by shifting activation of the channel to more depolarized voltages. The toxin binding sites may be situated on the S3-S4 extracellular linker of the channel. One, two, three or four toxin molecules may bind the Kv2.1/KCNB1 channel. May need to partition into the membrane in order to bind to the channel. Antibacterial activity is not observed.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Belongs to the huwentoxin-1 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainKnottin
   Molecular functionIon channel impairing toxin
Neurotoxin
Potassium channel impairing toxin
Toxin
Voltage-gated potassium channel impairing toxin
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionion channel inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3535Kappa-theraphotoxin-Gr1b
PRO_0000045013

Regions

Region4 – 63Involved in active face By similarity

Sites

Site31May be involved in interaction with voltage sensor By similarity
Site221May be involved in interaction with voltage sensor By similarity
Site301Involved in active face By similarity

Amino acid modifications

Disulfide bond2 ↔ 16 By similarity
Disulfide bond9 ↔ 21 By similarity
Disulfide bond15 ↔ 28 By similarity

Sequences

Sequence LengthMass (Da)Tools
P56853 [UniParc].

Last modified May 30, 2000. Version 1.
Checksum: 5601AC8A9B14C513

FASTA354,105
        10         20         30 
ECRYLFGGCK TTADCCKHLG CKFRDKYCAW DFTFS 

« Hide

References

[1]"An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantula."
Swartz K.J., MacKinnon R.
Neuron 15:941-949(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Venom.

Cross-references

3D structure databases

ProteinModelPortalP56853.
SMRP56853. Positions 1-35.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ArachnoServerAS000343. kappa-theraphotoxin-Gr1b.

Family and domain databases

InterProIPR011696. Ion_channel_inhibitory.
[Graphical view]
PfamPF07740. Toxin_12. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTXHN2_GRARO
AccessionPrimary (citable) accession number: P56853
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families