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P56852

- TXHN1_GRARO

UniProt

P56852 - TXHN1_GRARO

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Protein

Kappa-theraphotoxin-Gr1a

Gene
N/A
Organism
Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits Kv2.1/KCNB1 and Kv4.2/KCND2 voltage-gated potassium channels. Acts as a gating modifier by shifting channel openings to more depolarized voltages and acts via the occupancy of multiple binding sites on the channel. The toxin binding sites are situated on the S3-S4 extracellular linker of the channel. At least two hanatoxin molecules can occupy the Kv2.1/KCNB1 channel, and maybe more (three or four). Can also inhibit calcium channels (Cav2.1/CACNA1A). Needs to partition into the membrane in order to bind to the channel.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei3 – 31May be involved in interaction with voltage sensorBy similarity
Sitei22 – 221May be involved in interaction with voltage sensorBy similarity
Sitei30 – 301Involved in active faceBy similarity

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

TCDBi8.B.5.3.6. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Gr1a
Short name:
Kappa-TRTX-Gr1a
Alternative name(s):
Hanatoxin-1
Short name:
HaTx1
OrganismiGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Taxonomic identifieri432528 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

Organism-specific databases

ArachnoServeriAS000342. kappa-theraphotoxin-Gr1a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3535Kappa-theraphotoxin-Gr1aPRO_0000045012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 161 Publication
Disulfide bondi9 ↔ 211 Publication
Disulfide bondi15 ↔ 281 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
35
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 143Combined sources
Beta strandi17 – 215Combined sources
Turni23 – 253Combined sources
Beta strandi27 – 304Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D1HNMR-A1-35[»]
ProteinModelPortaliP56852.
SMRiP56852. Positions 1-35.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56852.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 63Involved in active faceBy similarity

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR011696. Ion_channel_inhibitory.
[Graphical view]
PfamiPF07740. Toxin_12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56852-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30 
ECRYLFGGCK TTSDCCKHLG CKFRDKYCAW DFTFS
Length:35
Mass (Da):4,121
Last modified:May 30, 2000 - v1
Checksum:iD401AC8A9B14DB12
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D1H NMR - A 1-35 [» ]
ProteinModelPortali P56852.
SMRi P56852. Positions 1-35.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 8.B.5.3.6. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ArachnoServeri AS000342. kappa-theraphotoxin-Gr1a.

Miscellaneous databases

EvolutionaryTracei P56852.

Family and domain databases

InterProi IPR011696. Ion_channel_inhibitory.
[Graphical view ]
Pfami PF07740. Toxin_12. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantula."
    Swartz K.J., MacKinnon R.
    Neuron 15:941-949(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Venom.
  2. "Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites."
    Swartz K.J., MacKinnon R.
    Neuron 18:665-673(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels."
    Swartz K.J., MacKinnon R.
    Neuron 18:675-682(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Gating modifier toxins reveal a conserved structural motif in voltage-gated Ca2+ and K+ channels."
    Li-Smerin Y., Swartz K.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:8585-8589(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Voltage-sensor activation with a tarantula toxin as cargo."
    Phillips L.R., Milescu M., Li-Smerin Y., Mindell J.A., Kim J.I., Swartz K.J.
    Nature 436:857-860(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MEMBRANE-PARTITIONING.
  6. "Gating modifier peptides as probes of pancreatic beta-cell physiology."
    Herrington J.
    Toxicon 49:231-238(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION ON PANCREATIC BETA-CELLS.
  7. "Solution structure of hanatoxin1, a gating modifier of voltage-dependent K(+) channels: common surface features of gating modifier toxins."
    Takahashi H., Kim J.I., Min H.J., Sato K., Swartz K.J., Shimada I.
    J. Mol. Biol. 297:771-780(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Entry informationi

Entry nameiTXHN1_GRARO
AccessioniPrimary (citable) accession number: P56852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3