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P56852 (TXHN1_GRARO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kappa-theraphotoxin-Gr1a

Short name=Kappa-TRTX-Gr1a
Alternative name(s):
Hanatoxin-1
Short name=HaTx1
OrganismGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Taxonomic identifier432528 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

Protein attributes

Sequence length35 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits Kv2.1/KCNB1 and Kv4.2/KCND2 voltage-gated potassium channels. Acts as a gating modifier by shifting channel openings to more depolarized voltages and acts via the occupancy of multiple binding sites on the channel. The toxin binding sites are situated on the S3-S4 extracellular linker of the channel. At least two hanatoxin molecules can occupy the Kv2.1/KCNB1 channel, and maybe more (three or four). Can also inhibit calcium channels (Cav2.1/CACNA1A). Needs to partition into the membrane in order to bind to the channel. Ref.2 Ref.3 Ref.4 Ref.6

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similarities

Belongs to the huwentoxin-1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3535Kappa-theraphotoxin-Gr1a
PRO_0000045012

Regions

Region4 – 63Involved in active face By similarity

Sites

Site31May be involved in interaction with voltage sensor By similarity
Site221May be involved in interaction with voltage sensor By similarity
Site301Involved in active face By similarity

Amino acid modifications

Disulfide bond2 ↔ 16 Ref.7
Disulfide bond9 ↔ 21 Ref.7
Disulfide bond15 ↔ 28 Ref.7

Secondary structure

......... 35
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56852 [UniParc].

Last modified May 30, 2000. Version 1.
Checksum: D401AC8A9B14DB12

FASTA354,121
        10         20         30 
ECRYLFGGCK TTSDCCKHLG CKFRDKYCAW DFTFS 

« Hide

References

[1]"An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantula."
Swartz K.J., MacKinnon R.
Neuron 15:941-949(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Venom.
[2]"Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites."
Swartz K.J., MacKinnon R.
Neuron 18:665-673(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels."
Swartz K.J., MacKinnon R.
Neuron 18:675-682(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Gating modifier toxins reveal a conserved structural motif in voltage-gated Ca2+ and K+ channels."
Li-Smerin Y., Swartz K.J.
Proc. Natl. Acad. Sci. U.S.A. 95:8585-8589(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Voltage-sensor activation with a tarantula toxin as cargo."
Phillips L.R., Milescu M., Li-Smerin Y., Mindell J.A., Kim J.I., Swartz K.J.
Nature 436:857-860(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MEMBRANE-PARTITIONING.
[6]"Gating modifier peptides as probes of pancreatic beta-cell physiology."
Herrington J.
Toxicon 49:231-238(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION ON PANCREATIC BETA-CELLS.
[7]"Solution structure of hanatoxin1, a gating modifier of voltage-dependent K(+) channels: common surface features of gating modifier toxins."
Takahashi H., Kim J.I., Min H.J., Sato K., Swartz K.J., Shimada I.
J. Mol. Biol. 297:771-780(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D1HNMR-A1-35[»]
ProteinModelPortalP56852.
SMRP56852. Positions 1-35.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB8.B.5.3.6. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ArachnoServerAS000342. kappa-theraphotoxin-Gr1a.

Family and domain databases

InterProIPR011696. Ion_channel_inhibitory.
[Graphical view]
PfamPF07740. Toxin_12. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP56852.

Entry information

Entry nameTXHN1_GRARO
AccessionPrimary (citable) accession number: P56852
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references