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Protein

Kappa-theraphotoxin-Gr1a

Gene
N/A
Organism
Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits Kv2.1/KCNB1 and Kv4.2/KCND2 voltage-gated potassium channels. Acts as a gating modifier by shifting channel openings to more depolarized voltages and acts via the occupancy of multiple binding sites on the channel. The toxin binding sites are situated on the S3-S4 extracellular linker of the channel. At least two hanatoxin molecules can occupy the Kv2.1/KCNB1 channel, and maybe more (three or four). Can also inhibit calcium channels (Cav2.1/CACNA1A). Needs to partition into the membrane in order to bind to the channel.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei3May be involved in interaction with voltage sensorBy similarity1
Sitei22May be involved in interaction with voltage sensorBy similarity1
Sitei30Involved in active faceBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

TCDBi8.B.5.3.6. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Gr1a
Short name:
Kappa-TRTX-Gr1a
Alternative name(s):
Hanatoxin-1
Short name:
HaTx1
OrganismiGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Taxonomic identifieri432528 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

Organism-specific databases

ArachnoServeriAS000342. kappa-theraphotoxin-Gr1a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000450121 – 35Kappa-theraphotoxin-Gr1aAdd BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi2 ↔ 161 Publication
Disulfide bondi9 ↔ 211 Publication
Disulfide bondi15 ↔ 281 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 14Combined sources3
Beta strandi17 – 21Combined sources5
Turni23 – 25Combined sources3
Beta strandi27 – 30Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D1HNMR-A1-35[»]
ProteinModelPortaliP56852.
SMRiP56852.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56852.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 6Involved in active faceBy similarity3

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. HaTx subfamily.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR011696. Huwentoxin-1.
[Graphical view]
PfamiPF07740. Toxin_12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
ECRYLFGGCK TTSDCCKHLG CKFRDKYCAW DFTFS
Length:35
Mass (Da):4,121
Last modified:May 30, 2000 - v1
Checksum:iD401AC8A9B14DB12
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D1HNMR-A1-35[»]
ProteinModelPortaliP56852.
SMRiP56852.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.5.3.6. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000342. kappa-theraphotoxin-Gr1a.

Miscellaneous databases

EvolutionaryTraceiP56852.

Family and domain databases

InterProiIPR011696. Huwentoxin-1.
[Graphical view]
PfamiPF07740. Toxin_12. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTXHN1_GRARO
AccessioniPrimary (citable) accession number: P56852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.