Beta-secretase 1 precursor - Rattus norvegicus (Rat)

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P56819 (BACE1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Beta-secretase 1
EC=3.4.23.46

Alternative name(s):
Aspartyl protease 2
Short name=ASP2
Short name=Asp 2
Beta-site amyloid precursor protein cleaving enzyme 1
Short name=Beta-site APP cleaving enzyme 1
Memapsin-2
Membrane-associated aspartic protease 2

Gene names

Name:	Bace1
Synonyms:	Bace

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase By similarity.
Catalytic activity	Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-\|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer amyloid precursor protein.
Enzyme regulation	Inhibited by RTN3 and RTN4 By similarity.
Subunit structure	Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. Interacts with SNX6 By similarity. Interacts with PCSK9 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein. Golgi apparatus › trans-Golgi network By similarity. Endoplasmic reticulum By similarity. Endosome By similarity. Cell surface By similarity. Cytoplasmic vesicle membrane By similarity. Note: Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface By similarity.
Domain	The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP By similarity.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	beta-amyloid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB axon Inferred from electronic annotation. Source: Compara cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Inferred from electronic annotation. Source: Compara trans-Golgi network Inferred from electronic annotation. Source: Compara
Molecular_function	aspartic-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Potential

Propeptide

22 – 45

Potential

PRO_0000025943

Chain

46 – 501

456

Beta-secretase 1

PRO_0000025944

Regions

Topological domain

22 – 457

436

Extracellular Potential

Transmembrane

458 – 478

Helical; Potential

Topological domain

479 – 501

Cytoplasmic Potential

Region

479 – 501

Interaction with RTN3 By similarity

Sites

Active site

By similarity

Active site

289

By similarity

Amino acid modifications

Glycosylation

153

N-linked (GlcNAc...) Potential

Glycosylation

172

N-linked (GlcNAc...) Potential

Glycosylation

223

N-linked (GlcNAc...) Potential

Glycosylation

354

N-linked (GlcNAc...) Potential

Disulfide bond

216 ↔ 420

By similarity

Disulfide bond

278 ↔ 443

By similarity

Disulfide bond

330 ↔ 380

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P56819 [UniParc].

Last modified May 30, 2000. Version 1.
Checksum: 24B445BC8BE87DE3
Show »

FASTA

501

55,807

        10         20         30         40         50         60 
MAPALRWLLL WVGSGMLPAQ GTHLGIRLPL RSGLAGPPLG LRLPRETDEE PEEPGRRGSF 

        70         80         90        100        110        120 
VEMVDNLRGK SGQGYYVEMT VGSPPQTLNI LVDTGSSNFA VGAAPHPFLH RYYQRQLSST 

       130        140        150        160        170        180 
YRDLRKSVYV PYTQGKWEGE LGTDLVSIPH GPNVTVRANI AAITESDKFF INGSNWEGIL 

       190        200        210        220        230        240 
GLAYAEIARP DDSLEPFFDS LVKQTHIPNI FSLQLCGAGF PLNQTEALAS VGGSMIIGGI 

       250        260        270        280        290        300 
DHSLYTGSLW YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK 

       310        320        330        340        350        360 
VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG EVTNQSFRIT 

       370        380        390        400        410        420 
ILPQQYLRPV EDVATSQDDC YKFAVSQSST GTVMGAVIME GFYVVFDRAR KRIGFAVSAC 

       430        440        450        460        470        480 
HVHDEFRTAA VEGPFVTADM EDCGYNIPQT DESTLMTIAY VMAAICALFM LPLCLMVCQW 

       490        500 
RCLRCLRHQH DDFADDISLL K

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References

[1]

"Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE."
Vassar R., Bennett B.D., Babu-Khan S., Kahn S., Mendiaz E.A., Denis P., Teplow D.B., Ross S., Amarante P., Loeloff R., Luo Y., Fisher S., Fuller J., Edenson S., Lile J., Jarosinski M.A., Biere A.L., Curran E.

Citron M.
Science 286:735-741(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF190727 mRNA. Translation: AAF04144.1.
IPI	IPI00196096.
RefSeq	NP_062077.1. NM_019204.1.
UniGene	Rn.207201.
3D structure databases
ProteinModelPortal	P56819.
SMR	P56819. Positions 55-447.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000022796.
Protein family/group databases
MEROPS	A01.004.
Proteomic databases
PRIDE	P56819.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000022796; ENSRNOP00000022796; ENSRNOG00000016847.
GeneID	29392.
KEGG	rno:29392.
UCSC	RGD:2191. rat.
Organism-specific databases
CTD	23621.
RGD	2191. Bace1.
Phylogenomic databases
eggNOG	NOG71700.
GeneTree	ENSGT00700000104165.
HOGENOM	HOG000036572.
HOVERGEN	HBG059578.
KO	K04521.
OrthoDB	EOG4SF95Q.
Gene expression databases
ArrayExpress	P56819.
Genevestigator	P56819.
GermOnline	ENSRNOG00000016847. Rattus norvegicus.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR009119. Pept_A1_BACE. IPR009120. Pept_A1_BACE1. IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR01816. BACE1. PR01815. BACEFAMILY. PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609013.

Entry information

Entry name

BACE1_RAT

Accession

Primary (citable) accession number: P56819

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents