P56753 (NDHH_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(P)H-quinone oxidoreductase subunit H, chloroplastic
EC=1.6.5.-

Alternative name(s):
NAD(P)H dehydrogenase subunit H
NADH-plastoquinone oxidoreductase 49 kDa subunit
NADH-plastoquinone oxidoreductase subunit H

Gene names

Name:	ndhH
Ordered Locus Names:	AtCg01110

Encoded on

Plastid; Chloroplast

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01358
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01358
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity. Interacts with the chaperonin CNP60B4 subunit. Ref.2
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side By similarity HAMAP MF_01358.
Miscellaneous	Folded specifically by a chaperonin Cpn60 complex containing at least 1 Cpn60 beta 4 subunit (Ref.2). HAMAP MF_01358
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on NADH or NADPH Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	NAD(P)H-quinone oxidoreductase subunit H, chloroplastic HAMAP MF_01358		PRO_0000118598

Sequences

	Sequence		Length	Mass (Da)
	P56753 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: 9782AD7CC942966B	FASTA	393	45,503
	10 20 30 40 50 60 MKRPVTGKDL MIVNMGPHHP SMHGVLRLIV TLDGEDVVDC EPILGYLHRG MEKIAENRAI 70 80 90 100 110 120 IQYLPYVTRW DYLATMFTEA ITVNGPEQLG NIQVPKRASY IRVIMLELSR IASHLLWLGP 130 140 150 160 170 180 FMADIGAQTP FFYIFREREF VYDLFEAATG MRMMHNFFRI GGIAADLPYG WIDKCLDFCD 190 200 210 220 230 240 YFLTEVVEYQ KLITRNPIFL ERVEGVGIIG GEEAINWGLS GPMLRASGIP WDLRKIDRYE 250 260 270 280 290 300 SYDEFEWEIQ WQKQGDSLAR YLVRLSEMTE SIKIIQQALE GLPGGPYENL ESRGFDRKRN 310 320 330 340 350 360 PEWNDFEYRF ISKKPSPTFE LSKQELYVRV EAPKGELGIF LIGDQSGFPW RWKIRPPGFI 370 380 390 NLQILPELVK RMKLADIMTI LGSIDIIMGE VDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete structure of the chloroplast genome of Arabidopsis thaliana." Sato S., Nakamura Y., Kaneko T., Asamizu E., Tabata S. DNA Res. 6:283-290(1999) [PubMed: 10574454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"A chaperonin subunit with unique structures is essential for folding of a specific substrate." Peng L., Fukao Y., Myouga F., Motohashi R., Shinozaki K., Shikanai T. PLoS Biol. 9:E1001040-E1001040(2011) [PubMed: 21483722] [Abstract] Cited for: INTERACTION WITH CNP60B4, FOLDING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000423 Genomic DNA. Translation: BAA84443.1.
IPI	IPI00538538.
RefSeq	NP_051115.1. NC_000932.1.
3D structure databases
ProteinModelPortal	P56753.
SMR	P56753. Positions 22-393.
ModBase	Search...
Protein-protein interaction databases
STRING	P56753.
Proteomic databases
PRIDE	P56753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	ATCG01110.1; ATCG01110.1; ATCG01110.
GeneID	844805.
GenomeReviews	Gene locus ATCG01110 in contig AP000423_GR.
KEGG	ath:ArthCp080.
NMPDR	fig|3702.1.peg.79.
Organism-specific databases
TAIR	AtCg01110.
Phylogenomic databases
eggNOG	KOG2870.
GeneTree	EPGT00050000011373.
HOGENOM	HBG459705.
PhylomeDB	P56753.
ProtClustDB	CHL00017.
Gene expression databases
Genevestigator	P56753.
GermOnline	ATCG01110. Arabidopsis thaliana.
Family and domain databases
HAMAP	MF_01358. NDH1_NuoD. [Tree]
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
KO	K05579.
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDHH_ARATH

Accession

Primary (citable) accession number: P56753

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program