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Protein

Claudin-8

Gene

CLDN8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tight-junction protein required for paracellular chloride transport in the kidney. Mediates recruitment of CLDN4 to tight junction in the kidney. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.By similarity

Miscellaneous

A protein of the expected size has been detected by antibody binding and Western blot in at least one of the analyzed tissues or cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-420029. Tight junction interactions.

Protein family/group databases

TCDBi1.H.1.1.7. the claudin tight junction (claudin1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-8
Gene namesi
Name:CLDN8Imported
ORF Names:UNQ779/PRO15731 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000156284.5.
HGNCiHGNC:2050. CLDN8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 81ExtracellularSequence analysisAdd BLAST53
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 117CytoplasmicSequence analysisAdd BLAST15
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Topological domaini139 – 166ExtracellularSequence analysisAdd BLAST28
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 225CytoplasmicSequence analysisAdd BLAST38

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

DisGeNETi9073.
OpenTargetsiENSG00000156284.
PharmGKBiPA26576.

Polymorphism and mutation databases

BioMutaiCLDN8.
DMDMi6685307.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447531 – 225Claudin-8Add BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP56748.
PeptideAtlasiP56748.
PRIDEiP56748.

PTM databases

iPTMnetiP56748.
PhosphoSitePlusiP56748.

Expressioni

Tissue specificityi

Expressed in the epididymis, mainly in the caput segment.1 Publication

Gene expression databases

BgeeiENSG00000156284.
CleanExiHS_CLDN8.
ExpressionAtlasiP56748. baseline and differential.
GenevisibleiP56748. HS.

Organism-specific databases

HPAiHPA060605.

Interactioni

Subunit structurei

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with CLDN4. Interacts with KLHL3.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114530. 4 interactors.
IntActiP56748. 6 interactors.
STRINGi9606.ENSP00000286809.

Structurei

3D structure databases

ProteinModelPortaliP56748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 225Interactions with TJP1, TJP2 and TJP3By similarity2

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJK4. Eukaryota.
ENOG410YQQY. LUCA.
GeneTreeiENSGT00760000118928.
HOVERGENiHBG000643.
InParanoidiP56748.
KOiK06087.
OMAiRGLMCAA.
OrthoDBiEOG091G0MX2.
PhylomeDBiP56748.
TreeFamiTF331936.

Family and domain databases

InterProiView protein in InterPro
IPR006187. Claudin.
IPR003926. Claudin8.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
PANTHERiPTHR12002. PTHR12002. 1 hit.
PTHR12002:SF24. PTHR12002:SF24. 1 hit.
PfamiView protein in Pfam
PF00822. PMP22_Claudin. 1 hit.
PROSITEiView protein in PROSITE
PS01346. CLAUDIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P56748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHALEIAG LFLGGVGMVG TVAVTVMPQW RVSAFIENNI VVFENFWEGL
60 70 80 90 100
WMNCVRQANI RMQCKIYDSL LALSPDLQAA RGLMCAASVM SFLAFMMAIL
110 120 130 140 150
GMKCTRCTGD NEKVKAHILL TAGIIFIITG MVVLIPVSWV ANAIIRDFYN
160 170 180 190 200
SIVNVAQKRE LGEALYLGWT TALVLIVGGA LFCCVFCCNE KSSSYRYSIP
210 220
SHRTTQKSYH TGKKSPSVYS RSQYV
Length:225
Mass (Da):24,845
Last modified:May 30, 2000 - v1
Checksum:iF7920E8E71682473
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170T → A in BAD97232 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04873925T → A. Corresponds to variant dbSNP:rs1557294Ensembl.1
Natural variantiVAR_024417129T → A. Corresponds to variant dbSNP:rs685967Ensembl.1
Natural variantiVAR_020387151S → P1 PublicationCorresponds to variant dbSNP:rs686364Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250711 Genomic DNA. Translation: CAB60615.1.
AY358707 mRNA. Translation: AAQ89070.1.
AK223512 mRNA. Translation: BAD97232.1.
AP001707 Genomic DNA. Translation: BAA95567.1.
CH471079 Genomic DNA. Translation: EAX09901.1.
CH471079 Genomic DNA. Translation: EAX09902.1.
BC020866 mRNA. Translation: AAH20866.1.
BC058004 mRNA. Translation: AAH58004.1.
CCDSiCCDS13587.1.
RefSeqiNP_955360.1. NM_199328.2.
UniGeneiHs.162209.

Genome annotation databases

EnsembliENST00000399899; ENSP00000382783; ENSG00000156284.
GeneIDi9073.
KEGGihsa:9073.
UCSCiuc002ynu.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCLD8_HUMAN
AccessioniPrimary (citable) accession number: P56748
Secondary accession number(s): D3DSE3, Q53EX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 22, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families