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P56748 (CLD8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Claudin-8
Gene names
Name:CLDN8
ORF Names:UNQ779/PRO1573
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a major role in tight junction-specific obliteration of the intercellular space By similarity.

Subunit structure

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 By similarity.

Subcellular location

Cell junctiontight junction. Cell membrane; Multi-pass membrane protein. Note: Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.

Tissue specificity

Expressed in the epididymis, mainly in the caput segment. Ref.7

Miscellaneous

A protein of the expected size has been detected by antibody binding and Western blot in at least one of the analyzed tissues or cells (Ref.8).

Sequence similarities

Belongs to the claudin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225Claudin-8
PRO_0000144753

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Potential
Topological domain29 – 8153Extracellular Potential
Transmembrane82 – 10221Helical; Potential
Topological domain103 – 11715Cytoplasmic Potential
Transmembrane118 – 13821Helical; Potential
Topological domain139 – 16628Extracellular Potential
Transmembrane167 – 18721Helical; Potential
Topological domain188 – 22538Cytoplasmic Potential
Region224 – 2252Interactions with TJP1, TJP2 and TJP3 By similarity

Natural variations

Natural variant251T → A.
Corresponds to variant rs1557294 [ dbSNP | Ensembl ].
VAR_048739
Natural variant1291T → A.
Corresponds to variant rs685967 [ dbSNP | Ensembl ].
VAR_024417
Natural variant1511S → P. Ref.3
Corresponds to variant rs686364 [ dbSNP | Ensembl ].
VAR_020387

Experimental info

Sequence conflict1701T → A in BAD97232. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P56748 [UniParc].

Last modified May 30, 2000. Version 1.
Checksum: F7920E8E71682473

FASTA22524,845
        10         20         30         40         50         60 
MATHALEIAG LFLGGVGMVG TVAVTVMPQW RVSAFIENNI VVFENFWEGL WMNCVRQANI 

        70         80         90        100        110        120 
RMQCKIYDSL LALSPDLQAA RGLMCAASVM SFLAFMMAIL GMKCTRCTGD NEKVKAHILL 

       130        140        150        160        170        180 
TAGIIFIITG MVVLIPVSWV ANAIIRDFYN SIVNVAQKRE LGEALYLGWT TALVLIVGGA 

       190        200        210        220 
LFCCVFCCNE KSSSYRYSIP SHRTTQKSYH TGKKSPSVYS RSQYV 

« Hide

References

« Hide 'large scale' references
[1]Keen T.J., Inglehearn C.F.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-151.
Tissue: Colon.
[4]"The DNA sequence of human chromosome 21."
Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. expand/collapse author list , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[7]"Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis."
Dube E., Chan P.T.K., Hermo L., Cyr D.G.
Biol. Reprod. 76:1034-1044(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Antibody-based protein profiling of the human chromosome 21."
Uhlen M., Oksvold P., Algenas C., Hamsten C., Fagerberg L., Klevebring D., Lundberg E., Odeberg J., Ponten F., Kondo T., Sivertsson A.
Mol. Cell. Proteomics 11:0-0(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: MISCELLANEOUS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ250711 Genomic DNA. Translation: CAB60615.1.
AY358707 mRNA. Translation: AAQ89070.1.
AK223512 mRNA. Translation: BAD97232.1.
AP001707 Genomic DNA. Translation: BAA95567.1.
CH471079 Genomic DNA. Translation: EAX09901.1.
CH471079 Genomic DNA. Translation: EAX09902.1.
BC020866 mRNA. Translation: AAH20866.1.
BC058004 mRNA. Translation: AAH58004.1.
CCDSCCDS13587.1.
RefSeqNP_955360.1. NM_199328.2.
UniGeneHs.162209.

3D structure databases

ProteinModelPortalP56748.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114530. 1 interaction.
STRING9606.ENSP00000286809.

Protein family/group databases

TCDB1.H.1.1.7. the claudin tight junction (claudin) family.

PTM databases

PhosphoSiteP56748.

Polymorphism databases

DMDM6685307.

Proteomic databases

PaxDbP56748.
PRIDEP56748.

Protocols and materials databases

DNASU9073.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000286809; ENSP00000286809; ENSG00000156284.
ENST00000399899; ENSP00000382783; ENSG00000156284.
GeneID9073.
KEGGhsa:9073.
UCSCuc002ynu.2. human.

Organism-specific databases

CTD9073.
GeneCardsGC21M031586.
HGNCHGNC:2050. CLDN8.
HPAHPA060605.
MIM611231. gene.
neXtProtNX_P56748.
PharmGKBPA26576.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40590.
HOVERGENHBG000643.
InParanoidP56748.
KOK06087.
OMAKCTRCTG.
OrthoDBEOG76HQ2T.
PhylomeDBP56748.
TreeFamTF331936.

Enzyme and pathway databases

ReactomeREACT_111155. Cell-Cell communication.

Gene expression databases

BgeeP56748.
CleanExHS_CLDN8.
GenevestigatorP56748.

Family and domain databases

InterProIPR006187. Claudin.
IPR003926. Claudin8.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERPTHR12002. PTHR12002. 1 hit.
PfamPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSPR01077. CLAUDIN.
PR01446. CLAUDIN8.
PROSITEPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCLDN8.
GenomeRNAi9073.
NextBio33997.
PROP56748.
SOURCESearch...

Entry information

Entry nameCLD8_HUMAN
AccessionPrimary (citable) accession number: P56748
Secondary accession number(s): D3DSE3, Q53EX7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM