Reviewed, UniProtKB/Swiss-Prot P56748 (CLD8_HUMAN)

Names and origin

Protein names

Recommended name:
    Claudin-8

Gene names

Name:	CLDN8
ORF Names:	UNQ779/PRO1573

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a major role in tight junction-specific obliteration of the intercellular space By similarity.
Subunit structure	Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 By similarity.
Subcellular location	Cell junction › tight junction. Cell membrane; Multi-pass membrane protein. Note: Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
Tissue specificity	Expressed in the epididymis, mainly in the caput segment. Ref.6
Sequence similarities	Belongs to the claudin family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Tight junction
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Gene Ontology (GO)
Biological process	calcium-independent cell-cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW tight junction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	identical protein binding Inferred from sequence or structural similarity. Source: UniProtKB structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Claudin-8		PRO_0000144753
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Potential
	Topological domain	29 – 81	53	Extracellular Potential
	Transmembrane	82 – 102	21	Potential
	Topological domain	103 – 117	15	Cytoplasmic Potential
	Transmembrane	118 – 138	21	Potential
	Topological domain	139 – 166	28	Extracellular Potential
	Transmembrane	167 – 187	21	Potential
	Topological domain	188 – 225	38	Cytoplasmic Potential
	Region	224 – 225	2	Interactions with TJP1, TJP2 and TJP3 By similarity
Natural variations
	Natural variant	25	1	T → A: dbSNP rs1557294.		VAR_048739
	Natural variant	129	1	T → A: dbSNP rs685967.		VAR_024417
	Natural variant	151	1	S → P: dbSNP rs686364. Ref.3		VAR_020387
Experimental info
	Sequence conflict	170	1	T → A in BAD97232. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P56748-1 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: F7920E8E71682473	FASTA	225	24,845
	10 20 30 40 50 60 MATHALEIAG LFLGGVGMVG TVAVTVMPQW RVSAFIENNI VVFENFWEGL WMNCVRQANI 70 80 90 100 110 120 RMQCKIYDSL LALSPDLQAA RGLMCAASVM SFLAFMMAIL GMKCTRCTGD NEKVKAHILL 130 140 150 160 170 180 TAGIIFIITG MVVLIPVSWV ANAIIRDFYN SIVNVAQKRE LGEALYLGWT TALVLIVGGA 190 200 210 220 LFCCVFCCNE KSSSYRYSIP SHRTTQKSYH TGKKSPSVYS RSQYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Keen T.J., Inglehearn C.F. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-151. Tissue: Colon.
[4]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis." Dube E., Chan P.T.K., Hermo L., Cyr D.G. Biol. Reprod. 76:1034-1044(2007) [PubMed: 17287494] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ250711 Genomic DNA. Translation: CAB60615.1. AY358707 mRNA. Translation: AAQ89070.1. AK223512 mRNA. Translation: BAD97232.1. AP001707 Genomic DNA. Translation: BAA95567.1. BC020866 mRNA. Translation: AAH20866.1. BC058004 mRNA. Translation: AAH58004.1.
IPI	IPI00011085.
RefSeq	NP_955360.1.
UniGene	Hs.162209
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P56748.
Proteomic databases
PRIDE	P56748.
Genome annotation databases
Ensembl	ENSG00000156284. Homo sapiens. [Contig view]
GeneID	9073.
KEGG	hsa:9073.
Organism-specific databases
GeneCards	GC21M030509.
H-InvDB	HIX0016053.
HGNC	HGNC:2050. CLDN8.
MIM	611231. gene.
PharmGKB	PA26576.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P56748.
HOVERGEN	P56748.
OMA	P56748. RGLMCAA.
Gene expression databases
ArrayExpress	P56748.
Bgee	P56748.
CleanEx	HS_CLDN8.
GermOnline	ENSG00000156284. Homo sapiens.
Family and domain databases
InterPro	IPR006187. Claudin. IPR003926. Claudin8. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. [Graphical view]
PANTHER	PTHR12002. Claudin. 1 hit.
Pfam	PF00822. PMP22_Claudin. 1 hit. [Graphical view]
PRINTS	PR01077. CLAUDIN. PR01446. CLAUDIN8.
PROSITE	PS01346. CLAUDIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	33997.
SOURCE	Search...

Entry information

Entry name

CLD8_HUMAN

Accession

Primary (citable) accession number: P56748
Secondary accession number(s): Q53EX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)