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Protein

Claudin-8

Gene

CLDN8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space.By similarity

GO - Molecular functioni

  1. identical protein binding Source: UniProtKB
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_19373. Tight junction interactions.

Protein family/group databases

TCDBi1.H.1.1.7. the claudin tight junction (claudin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-8
Gene namesi
Name:CLDN8
ORF Names:UNQ779/PRO1573
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:2050. CLDN8.

Subcellular locationi

  1. Cell junctiontight junction
  2. Cell membrane; Multi-pass membrane protein

  3. Note: Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Topological domaini29 – 8153ExtracellularSequence AnalysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence AnalysisAdd
BLAST
Topological domaini103 – 11715CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Topological domaini139 – 16628ExtracellularSequence AnalysisAdd
BLAST
Transmembranei167 – 18721HelicalSequence AnalysisAdd
BLAST
Topological domaini188 – 22538CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. endoplasmic reticulum Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB
  5. tight junction Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26576.

Polymorphism and mutation databases

BioMutaiCLDN8.
DMDMi6685307.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Claudin-8PRO_0000144753Add
BLAST

Proteomic databases

PaxDbiP56748.
PRIDEiP56748.

PTM databases

PhosphoSiteiP56748.

Expressioni

Tissue specificityi

Expressed in the epididymis, mainly in the caput segment.1 Publication

Gene expression databases

BgeeiP56748.
CleanExiHS_CLDN8.
GenevestigatoriP56748.

Organism-specific databases

HPAiHPA060605.

Interactioni

Subunit structurei

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC155Q8N6L03EBI-10215641,EBI-749265
SYNE4Q8N2053EBI-10215641,EBI-7131783

Protein-protein interaction databases

BioGridi114530. 3 interactions.
IntActiP56748. 2 interactions.
STRINGi9606.ENSP00000286809.

Structurei

3D structure databases

ProteinModelPortaliP56748.
SMRiP56748. Positions 1-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni224 – 2252Interactions with TJP1, TJP2 and TJP3By similarity

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40590.
GeneTreeiENSGT00760000118928.
HOVERGENiHBG000643.
InParanoidiP56748.
KOiK06087.
OMAiKCTRCTG.
OrthoDBiEOG76HQ2T.
PhylomeDBiP56748.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003926. Claudin8.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PTHR12002:SF24. PTHR12002:SF24. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01077. CLAUDIN.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHALEIAG LFLGGVGMVG TVAVTVMPQW RVSAFIENNI VVFENFWEGL
60 70 80 90 100
WMNCVRQANI RMQCKIYDSL LALSPDLQAA RGLMCAASVM SFLAFMMAIL
110 120 130 140 150
GMKCTRCTGD NEKVKAHILL TAGIIFIITG MVVLIPVSWV ANAIIRDFYN
160 170 180 190 200
SIVNVAQKRE LGEALYLGWT TALVLIVGGA LFCCVFCCNE KSSSYRYSIP
210 220
SHRTTQKSYH TGKKSPSVYS RSQYV
Length:225
Mass (Da):24,845
Last modified:May 30, 2000 - v1
Checksum:iF7920E8E71682473
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701T → A in BAD97232 (Ref. 3) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti25 – 251T → A.
Corresponds to variant rs1557294 [ dbSNP | Ensembl ].
VAR_048739
Natural varianti129 – 1291T → A.
Corresponds to variant rs685967 [ dbSNP | Ensembl ].
VAR_024417
Natural varianti151 – 1511S → P.1 Publication
Corresponds to variant rs686364 [ dbSNP | Ensembl ].
VAR_020387

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250711 Genomic DNA. Translation: CAB60615.1.
AY358707 mRNA. Translation: AAQ89070.1.
AK223512 mRNA. Translation: BAD97232.1.
AP001707 Genomic DNA. Translation: BAA95567.1.
CH471079 Genomic DNA. Translation: EAX09901.1.
CH471079 Genomic DNA. Translation: EAX09902.1.
BC020866 mRNA. Translation: AAH20866.1.
BC058004 mRNA. Translation: AAH58004.1.
CCDSiCCDS13587.1.
RefSeqiNP_955360.1. NM_199328.2.
UniGeneiHs.162209.

Genome annotation databases

EnsembliENST00000399899; ENSP00000382783; ENSG00000156284.
GeneIDi9073.
KEGGihsa:9073.
UCSCiuc002ynu.2. human.

Polymorphism and mutation databases

BioMutaiCLDN8.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250711 Genomic DNA. Translation: CAB60615.1.
AY358707 mRNA. Translation: AAQ89070.1.
AK223512 mRNA. Translation: BAD97232.1.
AP001707 Genomic DNA. Translation: BAA95567.1.
CH471079 Genomic DNA. Translation: EAX09901.1.
CH471079 Genomic DNA. Translation: EAX09902.1.
BC020866 mRNA. Translation: AAH20866.1.
BC058004 mRNA. Translation: AAH58004.1.
CCDSiCCDS13587.1.
RefSeqiNP_955360.1. NM_199328.2.
UniGeneiHs.162209.

3D structure databases

ProteinModelPortaliP56748.
SMRiP56748. Positions 1-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114530. 3 interactions.
IntActiP56748. 2 interactions.
STRINGi9606.ENSP00000286809.

Protein family/group databases

TCDBi1.H.1.1.7. the claudin tight junction (claudin) family.

PTM databases

PhosphoSiteiP56748.

Polymorphism and mutation databases

BioMutaiCLDN8.
DMDMi6685307.

Proteomic databases

PaxDbiP56748.
PRIDEiP56748.

Protocols and materials databases

DNASUi9073.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000399899; ENSP00000382783; ENSG00000156284.
GeneIDi9073.
KEGGihsa:9073.
UCSCiuc002ynu.2. human.

Organism-specific databases

CTDi9073.
GeneCardsiGC21M031586.
HGNCiHGNC:2050. CLDN8.
HPAiHPA060605.
MIMi611231. gene.
neXtProtiNX_P56748.
PharmGKBiPA26576.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG40590.
GeneTreeiENSGT00760000118928.
HOVERGENiHBG000643.
InParanoidiP56748.
KOiK06087.
OMAiKCTRCTG.
OrthoDBiEOG76HQ2T.
PhylomeDBiP56748.
TreeFamiTF331936.

Enzyme and pathway databases

ReactomeiREACT_19373. Tight junction interactions.

Miscellaneous databases

GeneWikiiCLDN8.
GenomeRNAii9073.
NextBioi33997.
PROiP56748.
SOURCEiSearch...

Gene expression databases

BgeeiP56748.
CleanExiHS_CLDN8.
GenevestigatoriP56748.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003926. Claudin8.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PTHR12002:SF24. PTHR12002:SF24. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01077. CLAUDIN.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Keen T.J., Inglehearn C.F.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-151.
    Tissue: Colon.
  4. "The DNA sequence of human chromosome 21."
    Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
    , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
    Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  7. "Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis."
    Dube E., Chan P.T.K., Hermo L., Cyr D.G.
    Biol. Reprod. 76:1034-1044(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. Cited for: MISCELLANEOUS.

Entry informationi

Entry nameiCLD8_HUMAN
AccessioniPrimary (citable) accession number: P56748
Secondary accession number(s): D3DSE3, Q53EX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: April 29, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

A protein of the expected size has been detected by antibody binding and Western blot in at least one of the analyzed tissues or cells.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.