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P56746

- CLD15_HUMAN

UniProt

P56746 - CLD15_HUMAN

Protein

Claudin-15

Gene

CLDN15

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (30 May 2000)
      Previous versions | rss
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    Functioni

    Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na+, Li+ and K+ ions, but selects against Cl- ions. Plays an important role in paracellular Na+ transport in the intestine and in Na+ homeostasis. Required for normal Na+-dependent intestinal nutrient uptake.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei55 – 551Important for Na(+)-selective paracellular ion transport
    Sitei64 – 641Important for Na(+)-selective paracellular ion transport
    Sitei68 – 681Important for the formation of tight-junction strand-like structuresBy similarity

    GO - Molecular functioni

    1. identical protein binding Source: UniProtKB
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. calcium-independent cell-cell adhesion Source: UniProtKB
    2. cell-cell junction organization Source: Reactome
    3. cell junction assembly Source: Reactome
    4. ion transport Source: UniProtKB-KW
    5. tight junction assembly Source: Reactome

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_19373. Tight junction interactions.

    Protein family/group databases

    TCDBi1.H.1.1.9. the claudin tight junction (claudin) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Claudin-15
    Gene namesi
    Name:CLDN15
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:2036. CLDN15.

    Subcellular locationi

    Cell junctiontight junction. Cell membrane; Multi-pass membrane protein
    Note: Tight junctions form continuous circumferential cell-cell contacts at the borders of apical and lateral cell membranes that seal the intercellular space and show up as strand-like structures in electron microscopy.

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. tight junction Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Tight junction

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi46 – 461E → K: No effect on charge selectivity for paracellular ion transport. 1 Publication
    Mutagenesisi55 – 551D → R: Reverses the charge selectivity for paracellular ion transport, favoring Cl(-) transport. 1 Publication
    Mutagenesisi64 – 641E → K: Impairs the charge selectivity for paracellular ion transport, favoring Cl(-) transport. 1 Publication

    Organism-specific databases

    PharmGKBiPA26562.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228Claudin-15PRO_0000144771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 ↔ 62By similarity

    Post-translational modificationi

    Palmitoylated.By similarity

    Keywords - PTMi

    Disulfide bond, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP56746.
    PRIDEiP56746.

    PTM databases

    PhosphoSiteiP56746.

    Expressioni

    Tissue specificityi

    Detected in colon (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP56746.
    BgeeiP56746.
    CleanExiHS_CLDN15.
    GenevestigatoriP56746.

    Organism-specific databases

    HPAiHPA053199.

    Interactioni

    Subunit structurei

    Can form linear homooligomers in the membrane, giving rise to tight junction strand-like structures.

    Protein-protein interaction databases

    BioGridi117296. 1 interaction.
    IntActiP56746. 1 interaction.
    STRINGi9606.ENSP00000308870.

    Structurei

    3D structure databases

    ProteinModelPortaliP56746.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 11CytoplasmicBy similarity
    Topological domaini25 – 7450ExtracellularBy similarityAdd
    BLAST
    Topological domaini100 – 11516CytoplasmicBy similarityAdd
    BLAST
    Topological domaini141 – 15919ExtracellularBy similarityAdd
    BLAST
    Topological domaini183 – 22846CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2423HelicalBy similarityAdd
    BLAST
    Transmembranei75 – 9925HelicalBy similarityAdd
    BLAST
    Transmembranei116 – 14025HelicalBy similarityAdd
    BLAST
    Transmembranei160 – 18223HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni146 – 1472Important for the formation of tight-junction strand-like structuresBy similarity

    Sequence similaritiesi

    Belongs to the claudin family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46136.
    HOGENOMiHOG000220937.
    HOVERGENiHBG000643.
    InParanoidiP56746.
    KOiK06087.
    OMAiIFENLWY.
    OrthoDBiEOG76HQ2T.
    PhylomeDBiP56746.
    TreeFamiTF331936.

    Family and domain databases

    InterProiIPR006187. Claudin.
    IPR008094. Claudin15.
    IPR017974. Claudin_CS.
    IPR004031. PMP22/EMP/MP20/Claudin.
    [Graphical view]
    PANTHERiPTHR12002. PTHR12002. 1 hit.
    PfamiPF00822. PMP22_Claudin. 1 hit.
    [Graphical view]
    PRINTSiPR01077. CLAUDIN.
    PR01718. CLAUDIN15.
    PROSITEiPS01346. CLAUDIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMAVETFGF FMATVGLLML GVTLPNSYWR VSTVHGNVIT TNTIFENLWF    50
    SCATDSLGVY NCWEFPSMLA LSGYIQACRA LMITAILLGF LGLLLGIAGL 100
    RCTNIGGLEL SRKAKLAATA GALHILAGIC GMVAISWYAF NITRDFFDPL 150
    YPGTKYELGP ALYLGWSASL ISILGGLCLC SACCCGSDED PAASARRPYQ 200
    APVSVMPVAT SDQEGDSSFG KYGRNAYV 228
    Length:228
    Mass (Da):24,356
    Last modified:May 30, 2000 - v1
    Checksum:iD502E2CD9116F4B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ245738 mRNA. Translation: CAB52587.1.
    AK056103 mRNA. Translation: BAG51627.1.
    CH471197 Genomic DNA. Translation: EAW50211.1.
    CCDSiCCDS5717.1.
    RefSeqiNP_001172009.1. NM_001185080.1.
    NP_055158.1. NM_014343.2.
    UniGeneiHs.38738.

    Genome annotation databases

    EnsembliENST00000308344; ENSP00000308870; ENSG00000106404.
    ENST00000401528; ENSP00000385300; ENSG00000106404.
    GeneIDi24146.
    KEGGihsa:24146.
    UCSCiuc003uyg.2. human.

    Polymorphism databases

    DMDMi6685306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ245738 mRNA. Translation: CAB52587.1 .
    AK056103 mRNA. Translation: BAG51627.1 .
    CH471197 Genomic DNA. Translation: EAW50211.1 .
    CCDSi CCDS5717.1.
    RefSeqi NP_001172009.1. NM_001185080.1.
    NP_055158.1. NM_014343.2.
    UniGenei Hs.38738.

    3D structure databases

    ProteinModelPortali P56746.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117296. 1 interaction.
    IntActi P56746. 1 interaction.
    STRINGi 9606.ENSP00000308870.

    Protein family/group databases

    TCDBi 1.H.1.1.9. the claudin tight junction (claudin) family.

    PTM databases

    PhosphoSitei P56746.

    Polymorphism databases

    DMDMi 6685306.

    Proteomic databases

    PaxDbi P56746.
    PRIDEi P56746.

    Protocols and materials databases

    DNASUi 24146.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308344 ; ENSP00000308870 ; ENSG00000106404 .
    ENST00000401528 ; ENSP00000385300 ; ENSG00000106404 .
    GeneIDi 24146.
    KEGGi hsa:24146.
    UCSCi uc003uyg.2. human.

    Organism-specific databases

    CTDi 24146.
    GeneCardsi GC07M100875.
    HGNCi HGNC:2036. CLDN15.
    HPAi HPA053199.
    MIMi 615778. gene.
    neXtProti NX_P56746.
    PharmGKBi PA26562.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG46136.
    HOGENOMi HOG000220937.
    HOVERGENi HBG000643.
    InParanoidi P56746.
    KOi K06087.
    OMAi IFENLWY.
    OrthoDBi EOG76HQ2T.
    PhylomeDBi P56746.
    TreeFami TF331936.

    Enzyme and pathway databases

    Reactomei REACT_19373. Tight junction interactions.

    Miscellaneous databases

    GeneWikii CLDN15.
    GenomeRNAii 24146.
    NextBioi 46839.
    PROi P56746.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P56746.
    Bgeei P56746.
    CleanExi HS_CLDN15.
    Genevestigatori P56746.

    Family and domain databases

    InterProi IPR006187. Claudin.
    IPR008094. Claudin15.
    IPR017974. Claudin_CS.
    IPR004031. PMP22/EMP/MP20/Claudin.
    [Graphical view ]
    PANTHERi PTHR12002. PTHR12002. 1 hit.
    Pfami PF00822. PMP22_Claudin. 1 hit.
    [Graphical view ]
    PRINTSi PR01077. CLAUDIN.
    PR01718. CLAUDIN15.
    PROSITEi PS01346. CLAUDIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Keen T.J., Inglehearn C.F.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Claudins create charge-selective channels in the paracellular pathway between epithelial cells."
      Colegio O.R., Van Itallie C.M., McCrea H.J., Rahner C., Anderson J.M.
      Am. J. Physiol. 283:C142-C142(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF GLU-46; ASP-55 AND GLU-64.
    5. "Reversal of charge selectivity in cation or anion-selective epithelial lines by expression of different claudins."
      Van Itallie C.M., Fanning A.S., Anderson J.M.
      Am. J. Physiol. 285:F1078-F1078(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCLD15_HUMAN
    AccessioniPrimary (citable) accession number: P56746
    Secondary accession number(s): B3KPB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3