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P56745 (CLD1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Claudin-1
Gene names
Name:Cldn1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions By similarity.

Subunit structure

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL By similarity.

Subcellular location

Cell junctiontight junction. Cell membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Detected in epididymis (at protein level). Detected in testis and epididymis. Ref.1

Sequence similarities

Belongs to the claudin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Claudin-1
PRO_0000144731

Regions

Topological domain1 – 77Cytoplasmic By similarity
Transmembrane8 – 2821Helical; Potential
Topological domain29 – 8153Extracellular Potential
Transmembrane82 – 10221Helical; Potential
Topological domain103 – 11513Cytoplasmic Potential
Transmembrane116 – 13621Helical; Potential
Topological domain137 – 16327Extracellular Potential
Transmembrane164 – 18421Helical; Potential
Topological domain185 – 21127Cytoplasmic By similarity
Region210 – 2112Interactions with TJP1, TJP2, TJP3 and INADL By similarity

Amino acid modifications

Disulfide bond54 ↔ 64 By similarity

Natural variations

Natural variant231V → D. Ref.1

Experimental info

Sequence conflict1331T → I in AAF04850. Ref.1
Sequence conflict1551V → I in AAH61992. Ref.2
Sequence conflict2061S → T in AAH61992. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P56745 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 5B123CE46C110D97

FASTA21122,843
        10         20         30         40         50         60 
MANAGLQLLG FILASLGWIG SIVSTALPQW KIYSYAGDNI VTAQAIYEGL WMSCVSQSTG 

        70         80         90        100        110        120 
QIQCKVFDSL LNLNSTLQAT RALMVIGILL GLIAIFVSTI GMKCMRCLED DEVQKMWMAV 

       130        140        150        160        170        180 
IGGIIFVISG LATLVATAWY GNRIVQEFYD PMTPVNARYE FGQALFTGWA AASLCLLGGA 

       190        200        210 
LLSCSCPRKT TSYPTPRPYP KPTPSSGKDY V 

« Hide

References

« Hide 'large scale' references
[1]"Claudin-1 is not restricted to tight junctions in the rat epididymis."
Gregory M., Dufresne J., Hermo L., Cyr D.
Endocrinology 142:854-863(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-23, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF195500 mRNA. Translation: AAF04850.1.
BC061992 mRNA. Translation: AAH61992.1.
RefSeqNP_113887.2. NM_031699.2.
XP_006248589.1. XM_006248527.1.
UniGeneRn.24293.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000002640.

PTM databases

PhosphoSiteP56745.

Proteomic databases

PRIDEP56745.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000002640; ENSRNOP00000002640; ENSRNOG00000001926.
GeneID65129.
KEGGrno:65129.
UCSCRGD:68422. rat.

Organism-specific databases

CTD9076.
RGD68422. Cldn1.

Phylogenomic databases

eggNOGNOG47316.
GeneTreeENSGT00740000115004.
HOGENOMHOG000220937.
HOVERGENHBG000643.
InParanoidP56745.
KOK06087.
OMAIYEGLWM.
OrthoDBEOG76HQ2T.
PhylomeDBP56745.
TreeFamTF331936.

Gene expression databases

GenevestigatorP56745.

Family and domain databases

InterProIPR006187. Claudin.
IPR003548. Claudin1.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERPTHR12002. PTHR12002. 1 hit.
PfamPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSPR01077. CLAUDIN.
PR01377. CLAUDIN1.
PROSITEPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613923.
PROP56745.

Entry information

Entry nameCLD1_RAT
AccessionPrimary (citable) accession number: P56745
Secondary accession number(s): Q6P6V9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 29, 2005
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families