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Protein

Claudin-1

Gene

Cldn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-1
Gene namesi
Name:Cldn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi68422. Cldn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicBy similarity
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Topological domaini29 – 8153ExtracellularSequence AnalysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence AnalysisAdd
BLAST
Topological domaini103 – 11513CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence AnalysisAdd
BLAST
Topological domaini137 – 16327ExtracellularSequence AnalysisAdd
BLAST
Transmembranei164 – 18421HelicalSequence AnalysisAdd
BLAST
Topological domaini185 – 21127CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Claudin-1PRO_0000144731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 64By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP56745.

PTM databases

PhosphoSiteiP56745.

Expressioni

Tissue specificityi

Detected in epididymis (at protein level). Detected in testis and epididymis.1 Publication

Gene expression databases

GenevisibleiP56745. RN.

Interactioni

Subunit structurei

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002640.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 2112Interactions with TJP1, TJP2, TJP3 and INADLBy similarity

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47316.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiP56745.
KOiK06087.
OMAiWKIYSYA.
OrthoDBiEOG76HQ2T.
PhylomeDBiP56745.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003548. Claudin1.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01077. CLAUDIN.
PR01377. CLAUDIN1.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANAGLQLLG FILASLGWIG SIVSTALPQW KIYSYAGDNI VTAQAIYEGL
60 70 80 90 100
WMSCVSQSTG QIQCKVFDSL LNLNSTLQAT RALMVIGILL GLIAIFVSTI
110 120 130 140 150
GMKCMRCLED DEVQKMWMAV IGGIIFVISG LATLVATAWY GNRIVQEFYD
160 170 180 190 200
PMTPVNARYE FGQALFTGWA AASLCLLGGA LLSCSCPRKT TSYPTPRPYP
210
KPTPSSGKDY V
Length:211
Mass (Da):22,843
Last modified:March 29, 2005 - v2
Checksum:i5B123CE46C110D97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331T → I in AAF04850 (PubMed:11159859).Curated
Sequence conflicti155 – 1551V → I in AAH61992 (PubMed:15489334).Curated
Sequence conflicti206 – 2061S → T in AAH61992 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 231V → D.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195500 mRNA. Translation: AAF04850.1.
BC061992 mRNA. Translation: AAH61992.1.
RefSeqiNP_113887.2. NM_031699.2.
UniGeneiRn.24293.

Genome annotation databases

EnsembliENSRNOT00000002640; ENSRNOP00000002640; ENSRNOG00000001926.
GeneIDi65129.
KEGGirno:65129.
UCSCiRGD:68422. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195500 mRNA. Translation: AAF04850.1.
BC061992 mRNA. Translation: AAH61992.1.
RefSeqiNP_113887.2. NM_031699.2.
UniGeneiRn.24293.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002640.

PTM databases

PhosphoSiteiP56745.

Proteomic databases

PRIDEiP56745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002640; ENSRNOP00000002640; ENSRNOG00000001926.
GeneIDi65129.
KEGGirno:65129.
UCSCiRGD:68422. rat.

Organism-specific databases

CTDi9076.
RGDi68422. Cldn1.

Phylogenomic databases

eggNOGiNOG47316.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiP56745.
KOiK06087.
OMAiWKIYSYA.
OrthoDBiEOG76HQ2T.
PhylomeDBiP56745.
TreeFamiTF331936.

Miscellaneous databases

NextBioi613923.
PROiP56745.

Gene expression databases

GenevisibleiP56745. RN.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003548. Claudin1.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01077. CLAUDIN.
PR01377. CLAUDIN1.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Claudin-1 is not restricted to tight junctions in the rat epididymis."
    Gregory M., Dufresne J., Hermo L., Cyr D.
    Endocrinology 142:854-863(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-23, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiCLD1_RAT
AccessioniPrimary (citable) accession number: P56745
Secondary accession number(s): Q6P6V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 29, 2005
Last modified: July 22, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.