Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Claudin-1

Gene

Cldn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • bicellular tight junction assembly Source: Ensembl
  • calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
  • cellular response to butyrate Source: RGD
  • cellular response to interferon-gamma Source: RGD
  • cellular response to lead ion Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • drug transport across blood-nerve barrier Source: RGD
  • establishment of blood-nerve barrier Source: RGD
  • establishment of endothelial intestinal barrier Source: RGD
  • establishment of skin barrier Source: UniProtKB
  • hyperosmotic salinity response Source: RGD
  • liver regeneration Source: RGD
  • positive regulation of bicellular tight junction assembly Source: RGD
  • protein heterooligomerization Source: Ensembl
  • protein homooligomerization Source: Ensembl
  • response to dexamethasone Source: RGD
  • response to ethanol Source: RGD
  • response to interleukin-18 Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to toxic substance Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-1
Gene namesi
Name:Cldn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi68422. Cldn1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicBy similarity7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 81ExtracellularSequence analysisAdd BLAST53
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 115CytoplasmicSequence analysisAdd BLAST13
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 163ExtracellularSequence analysisAdd BLAST27
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Topological domaini185 – 211CytoplasmicBy similarityAdd BLAST27

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • bicellular tight junction Source: UniProtKB
  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • lateral plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447311 – 211Claudin-1Add BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 64By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP56745.
PRIDEiP56745.

PTM databases

iPTMnetiP56745.
PhosphoSitePlusiP56745.

Expressioni

Tissue specificityi

Detected in epididymis (at protein level). Detected in testis and epididymis.1 Publication

Gene expression databases

BgeeiENSRNOG00000001926.
GenevisibleiP56745. RN.

Interactioni

Subunit structurei

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002640.

Structurei

3D structure databases

ProteinModelPortaliP56745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 211Interactions with TJP1, TJP2, TJP3 and PATJBy similarity2

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG63. Eukaryota.
ENOG4111GMP. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiP56745.
KOiK06087.
OMAiWKIYSYA.
OrthoDBiEOG091G0MX2.
PhylomeDBiP56745.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003548. Claudin1.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PTHR12002:SF92. PTHR12002:SF92. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01377. CLAUDIN1.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANAGLQLLG FILASLGWIG SIVSTALPQW KIYSYAGDNI VTAQAIYEGL
60 70 80 90 100
WMSCVSQSTG QIQCKVFDSL LNLNSTLQAT RALMVIGILL GLIAIFVSTI
110 120 130 140 150
GMKCMRCLED DEVQKMWMAV IGGIIFVISG LATLVATAWY GNRIVQEFYD
160 170 180 190 200
PMTPVNARYE FGQALFTGWA AASLCLLGGA LLSCSCPRKT TSYPTPRPYP
210
KPTPSSGKDY V
Length:211
Mass (Da):22,843
Last modified:March 29, 2005 - v2
Checksum:i5B123CE46C110D97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133T → I in AAF04850 (PubMed:11159859).Curated1
Sequence conflicti155V → I in AAH61992 (PubMed:15489334).Curated1
Sequence conflicti206S → T in AAH61992 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti23V → D.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195500 mRNA. Translation: AAF04850.1.
BC061992 mRNA. Translation: AAH61992.1.
RefSeqiNP_113887.2. NM_031699.2.
UniGeneiRn.24293.

Genome annotation databases

EnsembliENSRNOT00000002640; ENSRNOP00000002640; ENSRNOG00000001926.
GeneIDi65129.
KEGGirno:65129.
UCSCiRGD:68422. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195500 mRNA. Translation: AAF04850.1.
BC061992 mRNA. Translation: AAH61992.1.
RefSeqiNP_113887.2. NM_031699.2.
UniGeneiRn.24293.

3D structure databases

ProteinModelPortaliP56745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002640.

PTM databases

iPTMnetiP56745.
PhosphoSitePlusiP56745.

Proteomic databases

PaxDbiP56745.
PRIDEiP56745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002640; ENSRNOP00000002640; ENSRNOG00000001926.
GeneIDi65129.
KEGGirno:65129.
UCSCiRGD:68422. rat.

Organism-specific databases

CTDi9076.
RGDi68422. Cldn1.

Phylogenomic databases

eggNOGiENOG410IG63. Eukaryota.
ENOG4111GMP. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiP56745.
KOiK06087.
OMAiWKIYSYA.
OrthoDBiEOG091G0MX2.
PhylomeDBiP56745.
TreeFamiTF331936.

Miscellaneous databases

PROiP56745.

Gene expression databases

BgeeiENSRNOG00000001926.
GenevisibleiP56745. RN.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003548. Claudin1.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PTHR12002:SF92. PTHR12002:SF92. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01377. CLAUDIN1.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLD1_RAT
AccessioniPrimary (citable) accession number: P56745
Secondary accession number(s): Q6P6V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.