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Protein

Myosin-binding protein C, cardiac-type

Gene

Mybpc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi212 – 2121ZincBy similarity
Metal bindingi214 – 2141ZincBy similarity
Metal bindingi227 – 2271ZincBy similarity
Metal bindingi229 – 2291ZincBy similarity

GO - Molecular functioni

GO - Biological processi

  • cardiac muscle contraction Source: BHF-UCL
  • cell adhesion Source: UniProtKB-KW
  • regulation of striated muscle contraction Source: BHF-UCL
  • ventricular cardiac muscle tissue morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-binding protein C, cardiac-type
Short name:
Cardiac MyBP-C
Alternative name(s):
C-protein, cardiac muscle isoform
Gene namesi
Name:Mybpc3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1305950. Mybpc3.

Subcellular locationi

GO - Cellular componenti

  • A band Source: Ensembl
  • striated muscle myosin thick filament Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12741274Myosin-binding protein C, cardiac-typePRO_0000072695Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei47 – 471PhosphoserineBy similarity
Modified residuei117 – 1171PhosphothreonineCombined sources
Modified residuei279 – 2791PhosphoserineCombined sources
Modified residuei287 – 2871Phosphothreonine; by PKA and PKCBy similarity
Modified residuei288 – 2881PhosphoserineCombined sources
Modified residuei307 – 3071Phosphoserine; by PKABy similarity
Modified residuei312 – 3121PhosphoserineCombined sources
Modified residuei427 – 4271PhosphoserineBy similarity
Disulfide bondi436 ↔ 443PROSITE-ProRule annotation
Modified residuei459 – 4591PhosphoserineCombined sources
Modified residuei550 – 5501PhosphoserineBy similarity
Modified residuei607 – 6071PhosphothreonineCombined sources

Post-translational modificationi

Substrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction.
Polyubiquitinated.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP56741.
PRIDEiP56741.

PTM databases

PhosphoSiteiP56741.

Expressioni

Gene expression databases

GenevisibleiP56741. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016652.

Structurei

3D structure databases

ProteinModelPortaliP56741.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 9588Ig-like C2-type 1Add
BLAST
Domaini157 – 259103Ig-like C2-type 2Add
BLAST
Domaini361 – 45292Ig-like C2-type 3Add
BLAST
Domaini452 – 54695Ig-like C2-type 4Add
BLAST
Domaini645 – 765121Ig-like C2-type 5Add
BLAST
Domaini774 – 87097Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini872 – 96796Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini971 – 105989Ig-like C2-type 6Add
BLAST
Domaini1068 – 116396Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini1181 – 126989Ig-like C2-type 7Add
BLAST

Sequence similaritiesi

Belongs to the immunoglobulin superfamily. MyBP family.Curated
Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat

Phylogenomic databases

eggNOGiENOG410IFCI. Eukaryota.
ENOG4110AYI. LUCA.
GeneTreeiENSGT00840000129685.
InParanoidiP56741.
KOiK12568.
OMAiRVFSHNM.
OrthoDBiEOG7WX07H.
PhylomeDBiP56741.

Family and domain databases

Gene3Di2.60.40.10. 11 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF07679. I-set. 8 hits.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 8 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 8 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPGKRPVS AFTKKPRSVE VTAGSAAVFE AETERSGLKV QWQRDGSDIA
60 70 80 90 100
ANDKYGLAAE GKRHTLTVRD VGPDDQGSYA VIAGSSKVKF DLKVTEPAPP
110 120 130 140 150
EKAESAVAPT SMEAPETPKE VPALATQLEG NVSSPEGSVS VTQDGSVAGS
160 170 180 190 200
QGAPDDPIGL FLMRPQDGEV TVGGSIVFSA RVAGASLLKP PVVKWFKGKW
210 220 230 240 250
VDLSSKVGQH LQLHDSYDRA SKVYLFELHI TDAQATSAGG YRCEVSTKDK
260 270 280 290 300
FDSCNFNLTV HEAIGSGDLD LRSAFRRTSL AGTGRRTSDS HEDAGTLDFS
310 320 330 340 350
SLLKKSSFRR DSKLEAPAEE DVWEILRQAP PSEYERIAFQ HGVTDLRGML
360 370 380 390 400
KRLKGMKHDE KKSTAFQKKL EPAYQVNKGH KIRLTVELAD PDAEVKWLKN
410 420 430 440 450
GQEIQMSGRY IFESIGAKRT LTISQCSLAD DAAYQCVVGG EKCSTELFVK
460 470 480 490 500
EPPVLITRSL EDQLVMVGQR VEFECEVSEE GAQVKWLKDG VELTREETFK
510 520 530 540 550
YRFKKDGRKH HLIINEATLE DAGHYAVRTS GGQALAELIV QEKKLEVYQS
560 570 580 590 600
IADLAVGAKD QAVFKCEVSD ENVRGVWLKN GKELVPDNRI KVSHIGRVHK
610 620 630 640 650
LTIDDVTPAD EADYSFVPEG FACNLSAKLH FMEVKIDFVP RQEPPKIHLD
660 670 680 690 700
CPGSTPDTIV VVAGNKLRLD VPISGDPAPT VIWQKTITQG KKASAGPPPG
710 720 730 740 750
APEDAGADEE WVFDKKLLCE TEGRVRVETT KDRSVFTVEG AEKEDEGVYT
760 770 780 790 800
VTVKNPVGED QVNLTVKVID VPDAPAAPKI SNVGEDSCIV QWEPPAYDGG
810 820 830 840 850
QPVLGYILER KKKKSYRWMR LNFDLLRELS HEARRMIEGV AYEMRVYAVN
860 870 880 890 900
AVGMSRPSPA SQPFMPIGPP GEPTHLTVED VSDTTVSLKW RPPERVGAGG
910 920 930 940 950
LDGYSVEYCQ EGCSEWVTAL QGLTERTSLL VKDLPTGARL LFRVRAHNVA
960 970 980 990 1000
GPGGPIITKE PVTVQEILQR PRLQLPRHLR QTIQKKVGEP VNLLIPFQGK
1010 1020 1030 1040 1050
PRPQVTWTKE GQPLAGEEVS IRNSPTDTIL FIRAAHRTHS GTYQVTVRIE
1060 1070 1080 1090 1100
NMEDKATLVL QIVDKPSPPL DIRVVETWGF SVALEWKPPQ DDGNTEIWGY
1110 1120 1130 1140 1150
TVQKADKKTM EWFTVLEHYR QTHCVVSELI IGNGYYFRVF SHNMVGSSDR
1160 1170 1180 1190 1200
AAATKEPIFI PRPGITYEPP KYKALDFSEA PSFTQPLTNR SIIAGYNAIL
1210 1220 1230 1240 1250
CCAVRGSPKP KISWFKNGLD LGEDARFRMF CKQGVLTLEI RKPCPYDGGV
1260 1270
YVCRATNLQG EAQCECRLEV RVPQ
Length:1,274
Mass (Da):140,762
Last modified:February 8, 2011 - v2
Checksum:i6669E4F41B52B9E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 3061S → RD no nucleotide entry (PubMed:9784245).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL79508.1.
RefSeqiNP_001099960.1. NM_001106490.1.
UniGeneiRn.162668.

Genome annotation databases

EnsembliENSRNOT00000016652; ENSRNOP00000016652; ENSRNOG00000012307.
GeneIDi295929.
KEGGirno:295929.
UCSCiRGD:1305950. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL79508.1.
RefSeqiNP_001099960.1. NM_001106490.1.
UniGeneiRn.162668.

3D structure databases

ProteinModelPortaliP56741.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016652.

PTM databases

PhosphoSiteiP56741.

Proteomic databases

PaxDbiP56741.
PRIDEiP56741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016652; ENSRNOP00000016652; ENSRNOG00000012307.
GeneIDi295929.
KEGGirno:295929.
UCSCiRGD:1305950. rat.

Organism-specific databases

CTDi4607.
RGDi1305950. Mybpc3.

Phylogenomic databases

eggNOGiENOG410IFCI. Eukaryota.
ENOG4110AYI. LUCA.
GeneTreeiENSGT00840000129685.
InParanoidiP56741.
KOiK12568.
OMAiRVFSHNM.
OrthoDBiEOG7WX07H.
PhylomeDBiP56741.

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.

Miscellaneous databases

NextBioi640341.
PROiP56741.

Gene expression databases

GenevisibleiP56741. RN.

Family and domain databases

Gene3Di2.60.40.10. 11 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF07679. I-set. 8 hits.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 8 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 8 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Cardiac myosin-binding protein C (MyBP-C): identification of protein kinase A and protein kinase C phosphorylation sites."
    Mohamed A.S., Dignam J.D., Schlender K.K.
    Arch. Biochem. Biophys. 358:313-319(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 276-310.
    Tissue: Heart muscle.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-117; SER-279; SER-288; SER-312; SER-459 AND THR-607, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMYPC_RAT
AccessioniPrimary (citable) accession number: P56741
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 8, 2011
Last modified: May 11, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.