Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P56729

- SUIS_PIG

UniProt

P56729 - SUIS_PIG

Protein

Sucrase-isomaltase, intestinal

Gene

SI

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides By similarity.By similarity

    Catalytic activityi

    Hydrolysis of sucrose and maltose by an alpha-D-glucosidase-type action.
    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

    GO - Molecular functioni

    1. oligo-1,6-glucosidase activity Source: UniProtKB-EC
    2. sucrose alpha-glucosidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sucrase-isomaltase, intestinal
    Cleaved into the following 2 chains:
    Gene namesi
    Name:SI
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Apical cell membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Brush border.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – ›38›37IsomaltasePRO_0000018554Add
    BLAST
    Chaini39 – ›62›24SucrasePRO_0000018555Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei7 – 71Phosphoserine; by PKABy similarity
    Modified residuei59 – 591SulfotyrosineSequence Analysis

    Post-translational modificationi

    The precursor is proteolytically cleaved when exposed to pancreatic proteases in the intestinal lumen.
    Sulfated.1 Publication

    Keywords - PTMi

    Phosphoprotein, Sulfation

    Interactioni

    Subunit structurei

    The resulting sucrase and isomaltase subunits stay associated with one another in a complex by non-covalent linkages.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – ›38›6LumenalSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3220Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.Curated

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Sequencei

    Sequence statusi: Fragments.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P56729-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARKKFSGLE IXLIVLFAIV LSIAIALVVV XASKXPAVIK LPSDPIPTLR   50
    MEMTYHTDYM LE 62
    Length:62
    Mass (Da):6,893
    Last modified:January 23, 2007 - v2
    Checksum:i7D4E3C6AFC5AFB8B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence uncertaintyi33 – 331
    Sequence uncertaintyi34 – 341
    Non-adjacent residuesi38 – 392Curated
    Sequence uncertaintyi51 – 511M or V
    Sequence uncertaintyi53 – 531M or V
    Sequence uncertaintyi54 – 541T or K
    Sequence uncertaintyi57 – 571T or K
    Sequence uncertaintyi60 – 601M or V
    Non-terminal residuei62 – 621

    Sequence databases

    PIRiA25987.

    Cross-referencesi

    Sequence databases

    PIRi A25987.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "N-terminal sequences of pig intestinal sucrase-isomaltase and pro-sucrase-isomaltase. Implications for the biosynthesis and membrane insertion of pro-sucrase-isomaltase."
      Sjoestroem H., Noren O., Christiansen L.A., Wacker H., Spiess M., Bigler-Meier B., Rickli E.E., Semenza G.
      FEBS Lett. 148:321-325(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-62.
    2. "Tyrosine sulfation, a post-translational modification of microvillar enzymes in the small intestinal enterocyte."
      Danielsen E.M.
      EMBO J. 6:2891-2896(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: SULFATION.

    Entry informationi

    Entry nameiSUIS_PIG
    AccessioniPrimary (citable) accession number: P56729
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3