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P56726

- SMO_MOUSE

UniProt

P56726 - SMO_MOUSE

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Protein

Smoothened homolog

Gene
Smo, Smoh
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia By similarity.

GO - Molecular functioni

  1. drug binding Source: UniProtKB
  2. G-protein coupled receptor activity Source: UniProtKB-KW
  3. PDZ domain binding Source: RefGenome
  4. Wnt-activated receptor activity Source: RefGenome
  5. Wnt-protein binding Source: RefGenome

GO - Biological processi

  1. adenohypophysis development Source: RefGenome
  2. anterior/posterior pattern specification Source: MGI
  3. atrial septum morphogenesis Source: MGI
  4. axon extension involved in axon guidance Source: RefGenome
  5. canonical Wnt signaling pathway Source: RefGenome
  6. cardioblast differentiation Source: RefGenome
  7. cell development Source: MGI
  8. cell fate specification Source: MGI
  9. cellular response to cholesterol Source: BHF-UCL
  10. central nervous system development Source: MGI
  11. central nervous system neuron differentiation Source: MGI
  12. cerebellar cortex morphogenesis Source: MGI
  13. ciliary receptor clustering involved in smoothened signaling pathway Source: RefGenome
  14. detection of cell density by contact stimulus involved in contact inhibition Source: UniProtKB
  15. determination of left/right asymmetry in lateral mesoderm Source: BHF-UCL
  16. determination of left/right symmetry Source: MGI
  17. developmental growth Source: MGI
  18. digestive tract development Source: MGI
  19. dorsal/ventral neural tube patterning Source: MGI
  20. dorsal/ventral pattern formation Source: MGI
  21. embryonic camera-type eye development Source: RefGenome
  22. embryonic digestive tract morphogenesis Source: RefGenome
  23. embryonic neurocranium morphogenesis Source: RefGenome
  24. embryonic organ development Source: MGI
  25. embryonic viscerocranium morphogenesis Source: RefGenome
  26. epithelial-mesenchymal cell signaling Source: MGI
  27. exocrine pancreas development Source: RefGenome
  28. facial nerve development Source: RefGenome
  29. floor plate formation Source: RefGenome
  30. forebrain morphogenesis Source: BHF-UCL
  31. gonad development Source: RefGenome
  32. hair follicle morphogenesis Source: MGI
  33. heart looping Source: BHF-UCL
  34. heart morphogenesis Source: MGI
  35. in utero embryonic development Source: MGI
  36. mammary gland epithelial cell differentiation Source: MGI
  37. mesenchymal to epithelial transition involved in metanephric renal vesicle formation Source: UniProtKB
  38. midgut development Source: BHF-UCL
  39. multicellular organism growth Source: MGI
  40. muscle cell fate commitment Source: RefGenome
  41. myoblast migration Source: MGI
  42. negative regulation of apoptotic process Source: MGI
  43. negative regulation of DNA binding Source: UniProtKB
  44. negative regulation of epithelial cell differentiation Source: MGI
  45. negative regulation of gene expression Source: UniProtKB
  46. negative regulation of hair follicle development Source: MGI
  47. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  48. neural crest cell migration Source: MGI
  49. neuron fate commitment Source: RefGenome
  50. neuron projection regeneration Source: RefGenome
  51. odontogenesis of dentin-containing tooth Source: MGI
  52. ossification Source: MGI
  53. osteoblast differentiation Source: MGI
  54. otolith morphogenesis Source: RefGenome
  55. pancreas morphogenesis Source: MGI
  56. pattern specification process Source: MGI
  57. positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
  58. positive regulation of cell proliferation Source: MGI
  59. positive regulation of epithelial cell proliferation Source: MGI
  60. positive regulation of gene expression Source: UniProtKB
  61. positive regulation of hh target transcription factor activity Source: BHF-UCL
  62. positive regulation of mesenchymal cell proliferation Source: MGI
  63. positive regulation of neuroblast proliferation Source: MGI
  64. positive regulation of protein import into nucleus Source: MGI
  65. positive regulation of smoothened signaling pathway Source: MGI
  66. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  67. protein localization to nucleus Source: MGI
  68. protein stabilization Source: MGI
  69. regulation of gene expression Source: MGI
  70. regulation of heart morphogenesis Source: BHF-UCL
  71. regulation of stem cell maintenance Source: MGI
  72. semicircular canal morphogenesis Source: RefGenome
  73. skeletal muscle fiber development Source: MGI
  74. smoothened signaling pathway Source: UniProtKB
  75. smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Source: MGI
  76. smoothened signaling pathway involved in ventral spinal cord patterning Source: BHF-UCL
  77. somite development Source: BHF-UCL
  78. spermatogenesis Source: RefGenome
  79. thalamus development Source: MGI
  80. type B pancreatic cell development Source: MGI
  81. vasculogenesis Source: MGI
  82. ventral midline determination Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Smoothened homolog
Short name:
SMO
Gene namesi
Name:Smo
Synonyms:Smoh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:108075. Smo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 237205Extracellular Reviewed predictionAdd
BLAST
Transmembranei238 – 25821Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini259 – 2657Cytoplasmic Reviewed prediction
Transmembranei266 – 28621Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini287 – 31832Extracellular Reviewed predictionAdd
BLAST
Transmembranei319 – 33921Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini340 – 36223Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei363 – 38321Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini384 – 40623Extracellular Reviewed predictionAdd
BLAST
Transmembranei407 – 42721Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini428 – 45528Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei456 – 47621Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini477 – 52852Extracellular Reviewed predictionAdd
BLAST
Transmembranei529 – 54921Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini550 – 793244Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. caveola Source: Ensembl
  2. ciliary membrane Source: MGI
  3. cilium Source: MGI
  4. cytoplasm Source: MGI
  5. Golgi apparatus Source: Ensembl
  6. integral component of membrane Source: UniProtKB-KW
  7. neuronal cell body Source: RefGenome
  8. plasma membrane Source: UniProtKB
  9. primary cilium Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed predictionAdd
BLAST
Chaini33 – 793761Smoothened homologPRO_0000013016Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi74 ↔ 138 By similarity
Disulfide bondi82 ↔ 131 By similarity
Disulfide bondi122 ↔ 158 By similarity
Disulfide bondi151 ↔ 173 By similarity
Glycosylationi192 – 1921N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi197 ↔ 217 By similarity
Disulfide bondi221 ↔ 299 By similarity
Disulfide bondi318 ↔ 394 By similarity
Disulfide bondi494 ↔ 511 By similarity
Glycosylationi497 – 4971N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP56726.
PRIDEiP56726.

PTM databases

PhosphoSiteiP56726.

Expressioni

Gene expression databases

ArrayExpressiP56726.
BgeeiP56726.
CleanExiMM_SMO.
GenevestigatoriP56726.

Interactioni

Subunit structurei

Homodimer By similarity. Interacts with ARRB2. Interacts with KIF7. Interacts with BBS5 and BBS7; the interactions are indicative for the association of SMO with the BBsome complex to facilitate ciliary localization of SMO.2 Publications

Protein-protein interaction databases

BioGridi235504. 1 interaction.
IntActiP56726. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP56726.
SMRiP56726. Positions 63-179, 194-557.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 185117FZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni542 – 57332Interaction with BBS5 and BBS7Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG312505.
HOGENOMiHOG000252964.
HOVERGENiHBG000352.
InParanoidiP56726.
KOiK06226.
OMAiEVCPLAP.
OrthoDBiEOG74J972.
TreeFamiTF106460.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
IPR026544. SMO.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF35. PTHR11309:SF35. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56726-1 [UniParc]FASTAAdd to Basket

« Hide

MAAGRPVRGP ELAPRRLLQL LLLVLLGGPG RGAALSGNVT GPGPHSASGS    50
SRRDVPVTSP PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS 100
DSQEEAHGKL VLWSGLRNAP RCWAVIQPLL CAVYMPKCEN DRVELPSRTL 150
CQATRGPCAI VERERGWPDF LRCTPDHFPE GCPNEVQNIK FNSSGQCEAP 200
LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA FGAVTGLCTL 250
FTLATFVADW RNSNRYPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR 300
ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL 350
GTTYQPLSGK TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN 400
YRYRAGFVLA PIGLVLIVGG YFLIRGVMTL FSIKSNHPGL LSEKAASKIN 450
ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN QAEWERSFRD YVLCQANVTI 500
GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV WTKATLLIWR 550
RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH 600
DGPVAGLAFD LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV 650
PPEEQANMWL VEAEISPELE KRLGRKKKRR KRKKEVCPLR PAPELHHSAP 700
VPATSAVPRL PQLPRQKCLV AANAWGTGES CRQGAWTLVS NPFCPEPSPH 750
QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN LMEAEILDAD SDF 793
Length:793
Mass (Da):87,450
Last modified:July 27, 2011 - v2
Checksum:iDEAD556A45F7C960
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541D → N no nucleotide entry 1 Publication
Sequence conflicti266 – 2661Y → L no nucleotide entry 1 Publication
Sequence conflicti690 – 6901R → G no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC069469 Genomic DNA. No translation available.
CCDSiCCDS19965.1.
PIRiJC5539.
RefSeqiNP_795970.3. NM_176996.4.
UniGeneiMm.29279.

Genome annotation databases

EnsembliENSMUST00000001812; ENSMUSP00000001812; ENSMUSG00000001761.
GeneIDi319757.
KEGGimmu:319757.
UCSCiuc009bef.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC069469 Genomic DNA. No translation available.
CCDSi CCDS19965.1.
PIRi JC5539.
RefSeqi NP_795970.3. NM_176996.4.
UniGenei Mm.29279.

3D structure databases

ProteinModelPortali P56726.
SMRi P56726. Positions 63-179, 194-557.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 235504. 1 interaction.
IntActi P56726. 4 interactions.

Chemistry

BindingDBi P56726.
ChEMBLi CHEMBL6080.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P56726.

Proteomic databases

PaxDbi P56726.
PRIDEi P56726.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001812 ; ENSMUSP00000001812 ; ENSMUSG00000001761 .
GeneIDi 319757.
KEGGi mmu:319757.
UCSCi uc009bef.2. mouse.

Organism-specific databases

CTDi 6608.
MGIi MGI:108075. Smo.

Phylogenomic databases

eggNOGi NOG312505.
HOGENOMi HOG000252964.
HOVERGENi HBG000352.
InParanoidi P56726.
KOi K06226.
OMAi EVCPLAP.
OrthoDBi EOG74J972.
TreeFami TF106460.

Miscellaneous databases

ChiTaRSi Smo. mouse.
NextBioi 395350.
PROi P56726.
SOURCEi Search...

Gene expression databases

ArrayExpressi P56726.
Bgeei P56726.
CleanExi MM_SMO.
Genevestigatori P56726.

Family and domain databases

Gene3Di 1.10.2000.10. 1 hit.
InterProi IPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
IPR026544. SMO.
[Graphical view ]
PANTHERi PTHR11309. PTHR11309. 1 hit.
PTHR11309:SF35. PTHR11309:SF35. 1 hit.
Pfami PF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view ]
PRINTSi PR00489. FRIZZLED.
SMARTi SM00063. FRI. 1 hit.
[Graphical view ]
SUPFAMi SSF63501. SSF63501. 1 hit.
PROSITEi PS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a mouse Smoothened cDNA and expression patterns of hedgehog signalling molecules during chondrogenesis and cartilage differentiation in clonal mouse EC cells, ATDC5."
    Akiyama H., Shigeno C., Hiraki Y., Shukunami C., Kohno H., Akagi M., Konishi J., Nakamura T.
    Biochem. Biophys. Res. Commun. 235:142-147(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2."
    Chen W., Ren X.R., Nelson C.D., Barak L.S., Chen J.K., Beachy P.A., de Sauvage F., Lefkowitz R.J.
    Science 306:2257-2260(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARRB2.
  4. "A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened."
    Seo S., Zhang Q., Bugge K., Breslow D.K., Searby C.C., Nachury M.V., Sheffield V.C.
    PLoS Genet. 7:E1002358-E1002358(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH THE BBSOME COMPLEX, INTERACTION WITH BBS5 AND BBS7.

Entry informationi

Entry nameiSMO_MOUSE
AccessioniPrimary (citable) accession number: P56726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi