Reviewed, UniProtKB/Swiss-Prot P56726 (SMO_MOUSE)

Names and origin

Protein names

Recommended name:
    Smoothened homolog
      Short name=SMO

Gene names

Name:	Smo
Synonyms:	Smoh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO).
Subunit structure	Interacts with ARRB2. Ref.2
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW non-G-protein coupled 7TM receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 793	761	Smoothened homolog		PRO_0000013016
Regions
	Topological domain	33 – 237	205	Extracellular Potential
	Transmembrane	238 – 258	21	1 Potential
	Topological domain	259 – 265	7	Cytoplasmic Potential
	Transmembrane	266 – 286	21	2 Potential
	Topological domain	287 – 318	32	Extracellular Potential
	Transmembrane	319 – 339	21	3 Potential
	Topological domain	340 – 362	23	Cytoplasmic Potential
	Transmembrane	363 – 383	21	4 Potential
	Topological domain	384 – 406	23	Extracellular Potential
	Transmembrane	407 – 427	21	5 Potential
	Topological domain	428 – 455	28	Cytoplasmic Potential
	Transmembrane	456 – 476	21	6 Potential
	Topological domain	477 – 528	52	Extracellular Potential
	Transmembrane	529 – 549	21	7 Potential
	Topological domain	550 – 793	244	Cytoplasmic Potential
	Domain	69 – 185	117	FZ
Amino acid modifications
	Modified residue	791	1	Phosphoserine By similarity
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Disulfide bond	74 ↔ 138		By similarity
	Disulfide bond	82 ↔ 131		By similarity
	Disulfide bond	122 ↔ 158		By similarity
	Disulfide bond	151 ↔ 173		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56726-1 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: 10B2767876171167	FASTA	793	87,300
	10 20 30 40 50 60 MAAGRPVRGP ELAPRRLLQL LLLVLLGGPG RGAALSGNVT GPGPHSASGS SRRNVPVTSP 70 80 90 100 110 120 PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS DSQEEAHGKL VLWSGLRNAP 130 140 150 160 170 180 RCWAVIQPLL CAVYMPKCEN DRVELPSRTL CQATRGPCAI VERERGWPDF LRCTPDHFPE 190 200 210 220 230 240 GCPNEVQNIK FNSSGQCEAP LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA 250 260 270 280 290 300 FGAVTGLCTL FTLATFVADW RNSNRLPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR 310 320 330 340 350 360 ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL GTTYQPLSGK 370 380 390 400 410 420 TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN YRYRAGFVLA PIGLVLIVGG 430 440 450 460 470 480 YFLIRGVMTL FSIKSNHPGL LSEKAASKIN ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN 490 500 510 520 530 540 QAEWERSFRD YVLCQANVTI GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV 550 560 570 580 590 600 WTKATLLIWR RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH 610 620 630 640 650 660 DGPVAGLAFD LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV PPEEQANMWL 670 680 690 700 710 720 VEAEISPELE KRLGRKKKRR KRKKEVCPLG PAPELHHSAP VPATSAVPRL PQLPRQKCLV 730 740 750 760 770 780 AANAWGTGES CRQGAWTLVS NPFCPEPSPH QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN 790 LMEAEILDAD SDF

References

[1]	"Cloning of a mouse Smoothened cDNA and expression patterns of hedgehog signalling molecules during chondrogenesis and cartilage differentiation in clonal mouse EC cells, ATDC5." Akiyama H., Shigeno C., Hiraki Y., Shukunami C., Kohno H., Akagi M., Konishi J., Nakamura T. Biochem. Biophys. Res. Commun. 235:142-147(1997) [PubMed: 9196051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2." Chen W., Ren X.R., Nelson C.D., Barak L.S., Chen J.K., Beachy P.A., de Sauvage F., Lefkowitz R.J. Science 306:2257-2260(2004) [PubMed: 15618519] [Abstract] Cited for: INTERACTION WITH ARRB2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
IPI	IPI00341032.
PIR	JC5539.
UniGene	Mm.29279
3D structure databases
SMR	P56726. Positions 72-181.
ModBase	Search...
Protein-protein interaction databases
STRING	P56726.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P56726.
Proteomic databases
PRIDE	P56726.
Genome annotation databases
Ensembl	ENSMUST00000001812; ENSMUSP00000001812; ENSMUSG00000001761; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:108075. Smo.
Phylogenomic databases
eggNOG	roNOG05761.
HOGENOM	HBG714469.
HOVERGEN	P56726.
InParanoid	P56726.
Gene expression databases
ArrayExpress	P56726.
Bgee	P56726.
CleanEx	MM_SMO.
Genevestigator	P56726.
GermOnline	ENSMUSG00000001761. Mus musculus.
Family and domain databases
InterPro	IPR000539. Frizzled. IPR020067. Frizzled-like_dom. IPR000024. Frizzled_Cys-rich. IPR020068. Frizzled_dom_subgr. IPR015526. Frizzled_related. IPR017981. GPCR_2-like. [Graphical view]
Gene3D	G3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHER	PTHR11309. Fz_related. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. PF01392. Fz. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
SMART	SM00063. FRI. 1 hit. [Graphical view]
PROSITE	PS50038. FZ. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

SMO_MOUSE

Accession

Primary (citable) accession number: P56726

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)