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P56721 (COLL_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor collier
Alternative name(s):
Transcription factor knot
Gene names
Name:kn
Synonyms:col
ORF Names:CG10197
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May act as a 'second-level regulator' of head patterning. Required for establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. Required for expression of the segment polarity genes hedgehog, engrailed and wingless, and the segment-identity genes CAP and collar in the intercalary segment. Required at the onset of the gastrulation for the correct formation of the mandibular segment. Ref.1 Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus Potential.

Tissue specificity

Its expression at the blastoderm stage is restricted to a single stripe of cells corresponding to part of the intercalary and mandibular segment primordia, possibly parasegment O. Ref.1

Developmental stage

Isoform COL1 is expressed from 3 hours of embryogenesis, with a peak of accumulation between 8 and 16 hours post-fertilization. Expression persists at very low level in first instar larvae and accumulates again in third instar larvae and pupae. Isoform COL2 is expressed after 8 hours of embryogenesis, peaks in first instar larvae and is present at low levels in third instar larvae and pupae. Ref.1

Sequence similarities

Belongs to the COE family.

Contains 1 IPT/TIG domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior head segmentation

Traceable author statement PubMed 15382142. Source: FlyBase

blastoderm segmentation

Traceable author statement PubMed 15221856. Source: FlyBase

dendrite morphogenesis

Inferred from mutant phenotype PubMed 18093520. Source: FlyBase

determination of muscle attachment site

Inferred from mutant phenotype PubMed 22200594. Source: FlyBase

embryonic development via the syncytial blastoderm

Inferred from mutant phenotype Ref.7. Source: FlyBase

head segmentation

Inferred from mutant phenotype Ref.7. Source: FlyBase

imaginal disc-derived wing morphogenesis

Traceable author statement PubMed 12717815. Source: FlyBase

imaginal disc-derived wing vein specification

Inferred from mutant phenotype PubMed 12183378. Source: FlyBase

innate immune response

Inferred from mutant phenotype PubMed 15314643. Source: FlyBase

larval somatic muscle development

Inferred from mutant phenotype PubMed 22200594. Source: FlyBase

muscle cell fate specification

Inferred from mutant phenotype PubMed 22200594. Source: FlyBase

pattern specification process

Traceable author statement PubMed 11377964. Source: FlyBase

positive regulation of JAK-STAT cascade

Inferred from mutant phenotype PubMed 16094372. Source: FlyBase

posterior head segmentation

Traceable author statement PubMed 15382142. Source: FlyBase

regulation of lamellocyte differentiation

Inferred from mutant phenotype PubMed 15314643. Source: FlyBase

regulation of transcription, DNA-templated

Inferred from mutant phenotype Ref.7. Source: FlyBase

response to symbiont

Inferred from mutant phenotype PubMed 15314643. Source: FlyBase

specification of segmental identity, intercalary segment

Inferred from mutant phenotype Ref.7. Source: FlyBase

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform COL1 (identifier: P56721-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform COL2 (identifier: P56721-2)

The sequence of this isoform differs from the canonical sequence as follows:
     529-575: MSAVSSTWHQ...AVSAATAAAV → RVSSLSFNPFALPTCNTQGYSTQLVTSTK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575Transcription factor collier
PRO_0000107823

Regions

Domain299 – 38284IPT/TIG
Zinc finger167 – 18620C5-type Potential
Region79 – 824Interaction with DNA By similarity
Region213 – 2208Interaction with DNA By similarity
Region252 – 2554Interaction with DNA By similarity

Sites

Site1791Interaction with DNA By similarity
Site1881Interaction with DNA By similarity

Natural variations

Alternative sequence529 – 57547MSAVS…TAAAV → RVSSLSFNPFALPTCNTQGY STQLVTSTK in isoform COL2.
VSP_001111

Experimental info

Sequence conflict354 – 3552RH → SD in X97803. Ref.1
Sequence conflict3571P → R in AAM50962. Ref.4
Sequence conflict3841Missing in AAM50962. Ref.4
Sequence conflict4351G → D in X97803. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform COL1 [UniParc].

Last modified October 11, 2004. Version 2.
Checksum: D15144D95BDCDFBC

FASTA57562,494
        10         20         30         40         50         60 
MEWGRKLYPS AVSGPRSAGG LMFGLPPTAA VDMNQPRGPM TSLKEEPLGS RWAMQPVVDQ 

        70         80         90        100        110        120 
SNLGIGRAHF EKQPPSNLRK SNFFHFVIAL YDRAGQPIEI ERTAFIGFIE KDSESDATKT 

       130        140        150        160        170        180 
NNGIQYRLQL LYANGARQEQ DIFVRLIDSV TKQAIIYEGQ DKNPEMCRVL LTHEVMCSRC 

       190        200        210        220        230        240 
CDKKSCGNRN ETPSDPVIID RFFLKFFLKC NQNCLKNAGN PRDMRRFQVV ISTQVAVDGP 

       250        260        270        280        290        300 
LLAISDNMFV HNNSKHGRRA KRLDTTEGTG NTSLSISGHP LAPDSTYDGL YPPLPVATPC 

       310        320        330        340        350        360 
IKAISPSEGW TTGGATVIIV GDNFFDGLQV VFGTMLVWSE LITSHAIRVQ TPPRHIPGVV 

       370        380        390        400        410        420 
EVTLSYKSKQ FCKGSPGRFV YVSALNEPTI DYGFQRLQKL IPRHPGDPEK LQKEIILKRA 

       430        440        450        460        470        480 
ADLVEALYSM PRSPGGSTGF NSYAGQLAVS VQDGSGQWTE DDYQRAQSSS VSPRGGYCSS 

       490        500        510        520        530        540 
ASTPHSSGGS YGATAASAAV AATANGYAPA PNMGTLSSSP GSVFNSTSMS AVSSTWHQAF 

       550        560        570 
VQHHHAATAH PHHHYPHPHQ PWHNPAVSAA TAAAV 

« Hide

Isoform COL2 [UniParc].

Checksum: 16E4EFC1AC05A3BC
Show »

FASTA55760,488

References

« Hide 'large scale' references
[1]"Collier, a novel regulator of Drosophila head development, is expressed in a single mitotic domain."
Crozatier M., Valle D., Dubois L., Ibnsouda S., Vincent A.
Curr. Biol. 6:707-718(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS COL1 AND COL2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM COL2).
Strain: Berkeley.
Tissue: Embryo.
[5]"The COE transcription factor Collier is a mediator of short-range Hedgehog-induced patterning of the Drosophila wing."
Vervoort M., Crozatier M., Valle D., Vincent A.
Curr. Biol. 9:632-639(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Requirement for the Drosophila COE transcription factor Collier in formation of an embryonic muscle: transcriptional response to notch signalling."
Crozatier M., Vincent A.
Development 126:1495-1504(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Head versus trunk patterning in the Drosophila embryo; collier requirement for formation of the intercalary segment."
Crozatier M., Valle D., Dubois L., Ibnsouda S., Vincent A.
Development 126:4385-4394(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97803 mRNA. No translation available.
AE013599 Genomic DNA. Translation: AAF58204.2.
AY119102 mRNA. Translation: AAM50962.1.
RefSeqNP_524813.2. NM_080074.3. [P56721-2]
NP_725419.2. NM_166070.2. [P56721-1]
UniGeneDm.1803.

3D structure databases

ProteinModelPortalP56721.
SMRP56721. Positions 60-431.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid69588. 37 interactions.
STRING7227.FBpp0111722.

Proteomic databases

PaxDbP56721.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0112809; FBpp0111721; FBgn0001319. [P56721-1]
GeneID45318.
KEGGdme:Dmel_CG10197.
UCSCCG10197-RA. d. melanogaster. [P56721-1]

Organism-specific databases

CTD45318.
FlyBaseFBgn0001319. kn.

Phylogenomic databases

eggNOGNOG259050.
GeneTreeENSGT00390000014051.
InParanoidP56721.
KOK09103.
OrthoDBEOG7J446V.
PhylomeDBP56721.

Enzyme and pathway databases

SignaLinkP56721.

Gene expression databases

BgeeP56721.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERPTHR10747. PTHR10747. 1 hit.
PfamPF01833. TIG. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view]
SUPFAMSSF81296. SSF81296. 1 hit.
PROSITEPS01345. COE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi45318.
NextBio838001.
PROP56721.

Entry information

Entry nameCOLL_DROME
AccessionPrimary (citable) accession number: P56721
Secondary accession number(s): Q8MS49, Q9V758
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase