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P56721

- COLL_DROME

UniProt

P56721 - COLL_DROME

Protein

Transcription factor collier

Gene

kn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    May act as a 'second-level regulator' of head patterning. Required for establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. Required for expression of the segment polarity genes hedgehog, engrailed and wingless, and the segment-identity genes CAP and collar in the intercalary segment. Required at the onset of the gastrulation for the correct formation of the mandibular segment.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei179 – 1791Interaction with DNABy similarity
    Sitei188 – 1881Interaction with DNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri167 – 18620C5-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. anterior head segmentation Source: FlyBase
    2. blastoderm segmentation Source: FlyBase
    3. dendrite morphogenesis Source: FlyBase
    4. determination of muscle attachment site Source: FlyBase
    5. embryonic development via the syncytial blastoderm Source: FlyBase
    6. head segmentation Source: FlyBase
    7. imaginal disc-derived wing morphogenesis Source: FlyBase
    8. imaginal disc-derived wing vein specification Source: FlyBase
    9. innate immune response Source: FlyBase
    10. larval somatic muscle development Source: FlyBase
    11. muscle cell fate specification Source: FlyBase
    12. pattern specification process Source: FlyBase
    13. positive regulation of JAK-STAT cascade Source: FlyBase
    14. posterior head segmentation Source: FlyBase
    15. regulation of lamellocyte differentiation Source: FlyBase
    16. regulation of transcription, DNA-templated Source: FlyBase
    17. response to symbiont Source: FlyBase
    18. specification of segmental identity, intercalary segment Source: FlyBase
    19. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    SignaLinkiP56721.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor collier
    Alternative name(s):
    Transcription factor knot
    Gene namesi
    Name:kn
    Synonyms:col
    ORF Names:CG10197
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0001319. kn.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575Transcription factor collierPRO_0000107823Add
    BLAST

    Proteomic databases

    PaxDbiP56721.

    Expressioni

    Tissue specificityi

    Its expression at the blastoderm stage is restricted to a single stripe of cells corresponding to part of the intercalary and mandibular segment primordia, possibly parasegment O.1 Publication

    Developmental stagei

    Isoform COL1 is expressed from 3 hours of embryogenesis, with a peak of accumulation between 8 and 16 hours post-fertilization. Expression persists at very low level in first instar larvae and accumulates again in third instar larvae and pupae. Isoform COL2 is expressed after 8 hours of embryogenesis, peaks in first instar larvae and is present at low levels in third instar larvae and pupae.1 Publication

    Gene expression databases

    BgeeiP56721.

    Interactioni

    Protein-protein interaction databases

    BioGridi69588. 37 interactions.
    STRINGi7227.FBpp0111722.

    Structurei

    3D structure databases

    ProteinModelPortaliP56721.
    SMRiP56721. Positions 60-431.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini299 – 38284IPT/TIGAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni79 – 824Interaction with DNABy similarity
    Regioni213 – 2208Interaction with DNABy similarity
    Regioni252 – 2554Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the COE family.Curated
    Contains 1 IPT/TIG domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri167 – 18620C5-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG259050.
    GeneTreeiENSGT00390000014051.
    InParanoidiP56721.
    KOiK09103.
    OrthoDBiEOG7J446V.
    PhylomeDBiP56721.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR003523. Transcription_factor_COE.
    IPR018350. Transcription_factor_COE_CS.
    [Graphical view]
    PANTHERiPTHR10747. PTHR10747. 1 hit.
    PfamiPF01833. TIG. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    SM00429. IPT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.
    PROSITEiPS01345. COE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform COL1 (identifier: P56721-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEWGRKLYPS AVSGPRSAGG LMFGLPPTAA VDMNQPRGPM TSLKEEPLGS    50
    RWAMQPVVDQ SNLGIGRAHF EKQPPSNLRK SNFFHFVIAL YDRAGQPIEI 100
    ERTAFIGFIE KDSESDATKT NNGIQYRLQL LYANGARQEQ DIFVRLIDSV 150
    TKQAIIYEGQ DKNPEMCRVL LTHEVMCSRC CDKKSCGNRN ETPSDPVIID 200
    RFFLKFFLKC NQNCLKNAGN PRDMRRFQVV ISTQVAVDGP LLAISDNMFV 250
    HNNSKHGRRA KRLDTTEGTG NTSLSISGHP LAPDSTYDGL YPPLPVATPC 300
    IKAISPSEGW TTGGATVIIV GDNFFDGLQV VFGTMLVWSE LITSHAIRVQ 350
    TPPRHIPGVV EVTLSYKSKQ FCKGSPGRFV YVSALNEPTI DYGFQRLQKL 400
    IPRHPGDPEK LQKEIILKRA ADLVEALYSM PRSPGGSTGF NSYAGQLAVS 450
    VQDGSGQWTE DDYQRAQSSS VSPRGGYCSS ASTPHSSGGS YGATAASAAV 500
    AATANGYAPA PNMGTLSSSP GSVFNSTSMS AVSSTWHQAF VQHHHAATAH 550
    PHHHYPHPHQ PWHNPAVSAA TAAAV 575
    Length:575
    Mass (Da):62,494
    Last modified:October 11, 2004 - v2
    Checksum:iD15144D95BDCDFBC
    GO
    Isoform COL2 (identifier: P56721-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         529-575: MSAVSSTWHQ...AVSAATAAAV → RVSSLSFNPFALPTCNTQGYSTQLVTSTK

    Show »
    Length:557
    Mass (Da):60,488
    Checksum:i16E4EFC1AC05A3BC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti354 – 3552RH → SD in X97803. (PubMed:8793297)Curated
    Sequence conflicti357 – 3571P → R in AAM50962. (PubMed:12537569)Curated
    Sequence conflicti384 – 3841Missing in AAM50962. (PubMed:12537569)Curated
    Sequence conflicti435 – 4351G → D in X97803. (PubMed:8793297)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei529 – 57547MSAVS…TAAAV → RVSSLSFNPFALPTCNTQGY STQLVTSTK in isoform COL2. 1 PublicationVSP_001111Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97803 mRNA. No translation available.
    AE013599 Genomic DNA. Translation: AAF58204.2.
    AY119102 mRNA. Translation: AAM50962.1.
    RefSeqiNP_524813.2. NM_080074.3. [P56721-2]
    NP_725419.2. NM_166070.2. [P56721-1]
    UniGeneiDm.1803.

    Genome annotation databases

    EnsemblMetazoaiFBtr0112809; FBpp0111721; FBgn0001319. [P56721-1]
    GeneIDi45318.
    KEGGidme:Dmel_CG10197.
    UCSCiCG10197-RA. d. melanogaster. [P56721-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97803 mRNA. No translation available.
    AE013599 Genomic DNA. Translation: AAF58204.2 .
    AY119102 mRNA. Translation: AAM50962.1 .
    RefSeqi NP_524813.2. NM_080074.3. [P56721-2 ]
    NP_725419.2. NM_166070.2. [P56721-1 ]
    UniGenei Dm.1803.

    3D structure databases

    ProteinModelPortali P56721.
    SMRi P56721. Positions 60-431.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 69588. 37 interactions.
    STRINGi 7227.FBpp0111722.

    Proteomic databases

    PaxDbi P56721.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0112809 ; FBpp0111721 ; FBgn0001319 . [P56721-1 ]
    GeneIDi 45318.
    KEGGi dme:Dmel_CG10197.
    UCSCi CG10197-RA. d. melanogaster. [P56721-1 ]

    Organism-specific databases

    CTDi 45318.
    FlyBasei FBgn0001319. kn.

    Phylogenomic databases

    eggNOGi NOG259050.
    GeneTreei ENSGT00390000014051.
    InParanoidi P56721.
    KOi K09103.
    OrthoDBi EOG7J446V.
    PhylomeDBi P56721.

    Enzyme and pathway databases

    SignaLinki P56721.

    Miscellaneous databases

    GenomeRNAii 45318.
    NextBioi 838001.
    PROi P56721.

    Gene expression databases

    Bgeei P56721.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR003523. Transcription_factor_COE.
    IPR018350. Transcription_factor_COE_CS.
    [Graphical view ]
    PANTHERi PTHR10747. PTHR10747. 1 hit.
    Pfami PF01833. TIG. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    SM00429. IPT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    PROSITEi PS01345. COE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Collier, a novel regulator of Drosophila head development, is expressed in a single mitotic domain."
      Crozatier M., Valle D., Dubois L., Ibnsouda S., Vincent A.
      Curr. Biol. 6:707-718(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS COL1 AND COL2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Embryo.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM COL2).
      Strain: Berkeley.
      Tissue: Embryo.
    5. "The COE transcription factor Collier is a mediator of short-range Hedgehog-induced patterning of the Drosophila wing."
      Vervoort M., Crozatier M., Valle D., Vincent A.
      Curr. Biol. 9:632-639(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Requirement for the Drosophila COE transcription factor Collier in formation of an embryonic muscle: transcriptional response to notch signalling."
      Crozatier M., Vincent A.
      Development 126:1495-1504(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Head versus trunk patterning in the Drosophila embryo; collier requirement for formation of the intercalary segment."
      Crozatier M., Valle D., Dubois L., Ibnsouda S., Vincent A.
      Development 126:4385-4394(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCOLL_DROME
    AccessioniPrimary (citable) accession number: P56721
    Secondary accession number(s): Q8MS49, Q9V758
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3