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Protein

Transcription factor collier

Gene

kn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May act as a 'second-level regulator' of head patterning. Required for establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. Required for expression of the segment polarity genes hedgehog, engrailed and wingless, and the segment-identity genes CAP and collar in the intercalary segment. Required at the onset of the gastrulation for the correct formation of the mandibular segment.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 186C5-typeSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • anterior head segmentation Source: FlyBase
  • blastoderm segmentation Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • determination of muscle attachment site Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • head segmentation Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein specification Source: FlyBase
  • innate immune response Source: FlyBase
  • larval somatic muscle development Source: FlyBase
  • muscle cell fate specification Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • posterior head segmentation Source: FlyBase
  • regulation of dendrite morphogenesis Source: FlyBase
  • regulation of gene expression Source: FlyBase
  • regulation of lamellocyte differentiation Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • response to symbiont Source: FlyBase
  • segment polarity determination Source: FlyBase
  • specification of segmental identity, intercalary segment Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventral cord development Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiP56721

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor collier
Alternative name(s):
Transcription factor knot
Gene namesi
Name:kn
Synonyms:col
ORF Names:CG10197
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001319 kn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078231 – 575Transcription factor collierAdd BLAST575

Proteomic databases

PaxDbiP56721
PRIDEiP56721

Expressioni

Tissue specificityi

Its expression at the blastoderm stage is restricted to a single stripe of cells corresponding to part of the intercalary and mandibular segment primordia, possibly parasegment O.1 Publication

Developmental stagei

Isoform COL1 is expressed from 3 hours of embryogenesis, with a peak of accumulation between 8 and 16 hours post-fertilization. Expression persists at very low level in first instar larvae and accumulates again in third instar larvae and pupae. Isoform COL2 is expressed after 8 hours of embryogenesis, peaks in first instar larvae and is present at low levels in third instar larvae and pupae.1 Publication

Gene expression databases

BgeeiFBgn0001319
ExpressionAtlasiP56721 baseline and differential
GenevisibleiP56721 DM

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei179Interaction with DNABy similarity1
Sitei188Interaction with DNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

BioGridi69588, 49 interactors
STRINGi7227.FBpp0111722

Structurei

3D structure databases

ProteinModelPortaliP56721
SMRiP56721
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini299 – 382IPT/TIGAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 82Interaction with DNABy similarity4
Regioni213 – 220Interaction with DNABy similarity8
Regioni252 – 255Interaction with DNABy similarity4

Sequence similaritiesi

Belongs to the COE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 186C5-typeSequence analysisAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3836 Eukaryota
ENOG410XQ9Z LUCA
GeneTreeiENSGT00390000014051
InParanoidiP56721
KOiK09103
OMAiIRLIDSM
OrthoDBiEOG091G0A9D
PhylomeDBiP56721

Family and domain databases

CDDicd01175 IPT_COE, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.3180, 1 hit
4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR032200 COE_DBD
IPR038173 COE_DBD_sf
IPR032201 COE_HLH
IPR038006 COE_IPT
IPR036638 HLH_DNA-bd_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR003523 Transcription_factor_COE
IPR018350 Transcription_factor_COE_CS
PANTHERiPTHR10747 PTHR10747, 1 hit
PfamiView protein in Pfam
PF16422 COE1_DBD, 1 hit
PF16423 COE1_HLH, 1 hit
PF01833 TIG, 1 hit
SMARTiView protein in SMART
SM00429 IPT, 1 hit
SUPFAMiSSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS01345 COE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform COL1 (identifier: P56721-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEWGRKLYPS AVSGPRSAGG LMFGLPPTAA VDMNQPRGPM TSLKEEPLGS
60 70 80 90 100
RWAMQPVVDQ SNLGIGRAHF EKQPPSNLRK SNFFHFVIAL YDRAGQPIEI
110 120 130 140 150
ERTAFIGFIE KDSESDATKT NNGIQYRLQL LYANGARQEQ DIFVRLIDSV
160 170 180 190 200
TKQAIIYEGQ DKNPEMCRVL LTHEVMCSRC CDKKSCGNRN ETPSDPVIID
210 220 230 240 250
RFFLKFFLKC NQNCLKNAGN PRDMRRFQVV ISTQVAVDGP LLAISDNMFV
260 270 280 290 300
HNNSKHGRRA KRLDTTEGTG NTSLSISGHP LAPDSTYDGL YPPLPVATPC
310 320 330 340 350
IKAISPSEGW TTGGATVIIV GDNFFDGLQV VFGTMLVWSE LITSHAIRVQ
360 370 380 390 400
TPPRHIPGVV EVTLSYKSKQ FCKGSPGRFV YVSALNEPTI DYGFQRLQKL
410 420 430 440 450
IPRHPGDPEK LQKEIILKRA ADLVEALYSM PRSPGGSTGF NSYAGQLAVS
460 470 480 490 500
VQDGSGQWTE DDYQRAQSSS VSPRGGYCSS ASTPHSSGGS YGATAASAAV
510 520 530 540 550
AATANGYAPA PNMGTLSSSP GSVFNSTSMS AVSSTWHQAF VQHHHAATAH
560 570
PHHHYPHPHQ PWHNPAVSAA TAAAV
Length:575
Mass (Da):62,494
Last modified:October 11, 2004 - v2
Checksum:iD15144D95BDCDFBC
GO
Isoform COL2 (identifier: P56721-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     529-575: MSAVSSTWHQ...AVSAATAAAV → RVSSLSFNPFALPTCNTQGYSTQLVTSTK

Show »
Length:557
Mass (Da):60,488
Checksum:i16E4EFC1AC05A3BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354 – 355RH → SD in X97803 (PubMed:8793297).Curated2
Sequence conflicti357P → R in AAM50962 (PubMed:12537569).Curated1
Sequence conflicti384Missing in AAM50962 (PubMed:12537569).Curated1
Sequence conflicti435G → D in X97803 (PubMed:8793297).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001111529 – 575MSAVS…TAAAV → RVSSLSFNPFALPTCNTQGY STQLVTSTK in isoform COL2. 1 PublicationAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97803 mRNA No translation available.
AE013599 Genomic DNA Translation: AAF58204.2
AY119102 mRNA Translation: AAM50962.1
RefSeqiNP_524813.2, NM_080074.4 [P56721-2]
NP_725419.2, NM_166070.3 [P56721-1]
UniGeneiDm.1803

Genome annotation databases

EnsemblMetazoaiFBtr0087465; FBpp0086595; FBgn0001319 [P56721-2]
FBtr0112809; FBpp0111721; FBgn0001319 [P56721-1]
GeneIDi45318
KEGGidme:Dmel_CG10197
UCSCiCG10197-RA d. melanogaster [P56721-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCOLL_DROME
AccessioniPrimary (citable) accession number: P56721
Secondary accession number(s): Q8MS49, Q9V758
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 11, 2004
Last modified: June 20, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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