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Protein

Transcription factor collier

Gene

kn

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May act as a 'second-level regulator' of head patterning. Required for establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. Required for expression of the segment polarity genes hedgehog, engrailed and wingless, and the segment-identity genes CAP and collar in the intercalary segment. Required at the onset of the gastrulation for the correct formation of the mandibular segment.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 186C5-typeSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • anterior head segmentation Source: FlyBase
  • blastoderm segmentation Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • determination of muscle attachment site Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • head segmentation Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein specification Source: FlyBase
  • innate immune response Source: FlyBase
  • larval somatic muscle development Source: FlyBase
  • muscle cell fate specification Source: FlyBase
  • pattern specification process Source: FlyBase
  • positive regulation of JAK-STAT cascade Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • posterior head segmentation Source: FlyBase
  • regulation of dendrite morphogenesis Source: FlyBase
  • regulation of gene expression Source: FlyBase
  • regulation of lamellocyte differentiation Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • response to symbiont Source: FlyBase
  • segment polarity determination Source: FlyBase
  • specification of segmental identity, intercalary segment Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventral cord development Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiP56721.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor collier
Alternative name(s):
Transcription factor knot
Gene namesi
Name:kn
Synonyms:col
ORF Names:CG10197
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001319. kn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078231 – 575Transcription factor collierAdd BLAST575

Proteomic databases

PaxDbiP56721.
PRIDEiP56721.

Expressioni

Tissue specificityi

Its expression at the blastoderm stage is restricted to a single stripe of cells corresponding to part of the intercalary and mandibular segment primordia, possibly parasegment O.1 Publication

Developmental stagei

Isoform COL1 is expressed from 3 hours of embryogenesis, with a peak of accumulation between 8 and 16 hours post-fertilization. Expression persists at very low level in first instar larvae and accumulates again in third instar larvae and pupae. Isoform COL2 is expressed after 8 hours of embryogenesis, peaks in first instar larvae and is present at low levels in third instar larvae and pupae.1 Publication

Gene expression databases

BgeeiFBgn0001319.
ExpressionAtlasiP56721. differential.
GenevisibleiP56721. DM.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei179Interaction with DNABy similarity1
Sitei188Interaction with DNABy similarity1

Protein-protein interaction databases

BioGridi69588. 49 interactors.
STRINGi7227.FBpp0111722.

Structurei

3D structure databases

ProteinModelPortaliP56721.
SMRiP56721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini299 – 382IPT/TIGAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 82Interaction with DNABy similarity4
Regioni213 – 220Interaction with DNABy similarity8
Regioni252 – 255Interaction with DNABy similarity4

Sequence similaritiesi

Belongs to the COE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 186C5-typeSequence analysisAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3836. Eukaryota.
ENOG410XQ9Z. LUCA.
GeneTreeiENSGT00390000014051.
InParanoidiP56721.
KOiK09103.
OrthoDBiEOG091G0A9D.
PhylomeDBiP56721.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR032200. COE_DBD.
IPR032201. COE_HLH.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiView protein in Pfam
PF16422. COE1_DBD. 1 hit.
PF16423. COE1_HLH. 1 hit.
PF01833. TIG. 1 hit.
SMARTiView protein in SMART
SM00429. IPT. 1 hit.
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiView protein in PROSITE
PS01345. COE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform COL1 (identifier: P56721-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEWGRKLYPS AVSGPRSAGG LMFGLPPTAA VDMNQPRGPM TSLKEEPLGS
60 70 80 90 100
RWAMQPVVDQ SNLGIGRAHF EKQPPSNLRK SNFFHFVIAL YDRAGQPIEI
110 120 130 140 150
ERTAFIGFIE KDSESDATKT NNGIQYRLQL LYANGARQEQ DIFVRLIDSV
160 170 180 190 200
TKQAIIYEGQ DKNPEMCRVL LTHEVMCSRC CDKKSCGNRN ETPSDPVIID
210 220 230 240 250
RFFLKFFLKC NQNCLKNAGN PRDMRRFQVV ISTQVAVDGP LLAISDNMFV
260 270 280 290 300
HNNSKHGRRA KRLDTTEGTG NTSLSISGHP LAPDSTYDGL YPPLPVATPC
310 320 330 340 350
IKAISPSEGW TTGGATVIIV GDNFFDGLQV VFGTMLVWSE LITSHAIRVQ
360 370 380 390 400
TPPRHIPGVV EVTLSYKSKQ FCKGSPGRFV YVSALNEPTI DYGFQRLQKL
410 420 430 440 450
IPRHPGDPEK LQKEIILKRA ADLVEALYSM PRSPGGSTGF NSYAGQLAVS
460 470 480 490 500
VQDGSGQWTE DDYQRAQSSS VSPRGGYCSS ASTPHSSGGS YGATAASAAV
510 520 530 540 550
AATANGYAPA PNMGTLSSSP GSVFNSTSMS AVSSTWHQAF VQHHHAATAH
560 570
PHHHYPHPHQ PWHNPAVSAA TAAAV
Length:575
Mass (Da):62,494
Last modified:October 11, 2004 - v2
Checksum:iD15144D95BDCDFBC
GO
Isoform COL2 (identifier: P56721-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     529-575: MSAVSSTWHQ...AVSAATAAAV → RVSSLSFNPFALPTCNTQGYSTQLVTSTK

Show »
Length:557
Mass (Da):60,488
Checksum:i16E4EFC1AC05A3BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354 – 355RH → SD in X97803 (PubMed:8793297).Curated2
Sequence conflicti357P → R in AAM50962 (PubMed:12537569).Curated1
Sequence conflicti384Missing in AAM50962 (PubMed:12537569).Curated1
Sequence conflicti435G → D in X97803 (PubMed:8793297).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001111529 – 575MSAVS…TAAAV → RVSSLSFNPFALPTCNTQGY STQLVTSTK in isoform COL2. 1 PublicationAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97803 mRNA. No translation available.
AE013599 Genomic DNA. Translation: AAF58204.2.
AY119102 mRNA. Translation: AAM50962.1.
RefSeqiNP_524813.2. NM_080074.4. [P56721-2]
NP_725419.2. NM_166070.3. [P56721-1]
UniGeneiDm.1803.

Genome annotation databases

EnsemblMetazoaiFBtr0087465; FBpp0086595; FBgn0001319. [P56721-2]
FBtr0112809; FBpp0111721; FBgn0001319. [P56721-1]
GeneIDi45318.
KEGGidme:Dmel_CG10197.
UCSCiCG10197-RA. d. melanogaster. [P56721-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCOLL_DROME
AccessioniPrimary (citable) accession number: P56721
Secondary accession number(s): Q8MS49, Q9V758
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 11, 2004
Last modified: September 27, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families