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P56703 (WNT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proto-oncogene Wnt-3
Alternative name(s):
Proto-oncogene Int-4 homolog
Gene names
Name:WNT3
Synonyms:INT4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube By similarity.

Subunit structure

Interacts with PORCN By similarity. Interacts with WLS By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Post-translational modification

Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-80. Palmitoylation is necessary for proper trafficking to cell surface By similarity.

Involvement in disease

Tetraamelia, autosomal recessive (ARTTRA) [MIM:273395]: A rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   DiseaseProto-oncogene
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSpemann organizer formation at the anterior end of the primitive streak

Inferred from electronic annotation. Source: Ensembl

anterior/posterior axis specification

Inferred from electronic annotation. Source: Ensembl

axon guidance

Inferred from electronic annotation. Source: Ensembl

canonical Wnt signaling pathway

Inferred from direct assay PubMed 10557084. Source: UniProtKB

canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation

Inferred from mutant phenotype PubMed 20039315. Source: BHF-UCL

canonical Wnt signaling pathway involved in osteoblast differentiation

Inferred from mutant phenotype PubMed 20039315. Source: BHF-UCL

cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell morphogenesis

Inferred from mutant phenotype PubMed 15588944. Source: BHF-UCL

cellular response to retinoic acid

Inferred from sequence or structural similarity. Source: UniProtKB

dorsal/ventral axis specification

Inferred from electronic annotation. Source: Ensembl

embryonic forelimb morphogenesis

Inferred from electronic annotation. Source: Ensembl

embryonic hindlimb morphogenesis

Inferred from electronic annotation. Source: Ensembl

head morphogenesis

Inferred from electronic annotation. Source: Ensembl

limb bud formation

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

mammary gland epithelium development

Inferred from expression pattern PubMed 8168088. Source: UniProtKB

mesoderm formation

Inferred from electronic annotation. Source: Ensembl

negative regulation of axon extension involved in axon guidance

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of collateral sprouting in absence of injury

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi lumen

Traceable author statement. Source: Reactome

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Traceable author statement PubMed 18313787. Source: BHF-UCL

plasma membrane

Traceable author statement. Source: Reactome

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfrizzled binding

Inferred from physical interaction PubMed 10557084. Source: UniProtKB

receptor agonist activity

Inferred by curator PubMed 18044981. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FZD7O750843EBI-3644922,EBI-746917

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 355334Proto-oncogene Wnt-3
PRO_0000041416

Amino acid modifications

Lipidation801S-palmitoyl cysteine By similarity
Lipidation2121O-palmitoyl serine; by PORCN By similarity
Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P56703 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: 85D15F2C7884A64F

FASTA35539,645
        10         20         30         40         50         60 
MEPHLLGLLL GLLLGGTRVL AGYPIWWSLA LGQQYTSLGS QPLLCGSIPG LVPKQLRFCR 

        70         80         90        100        110        120 
NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI FGPVLDKATR ESAFVHAIAS 

       130        140        150        160        170        180 
AGVAFAVTRS CAEGTSTICG CDSHHKGPPG EGWKWGGCSE DADFGVLVSR EFADARENRP 

       190        200        210        220        230        240 
DARSAMNKHN NEAGRTTILD HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS 

       250        260        270        280        290        300 
ASEMVVEKHR ESRGWVETLR AKYSLFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC 

       310        320        330        340        350 
NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCIFHWCCYV SCQECIRIYD VHTCK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of six novel human WNT genes."
Testa T.T., Mossakowska D.E., Carter P.S., Hu E., Zhu Y., Kelsell D.P., Murdock P.R., Herrity N.C., Lewis C.J., Cross D.A., Culbert A.A., Reith A.D., Barnes M.R.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning and characterization of human WNT3."
Katoh M.
Int. J. Oncol. 19:977-982(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene."
Roelink H., Wang J., Black D.M., Solomon E., Nusse R.
Genomics 17:790-792(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-333.
[5]"Homozygous WNT3 mutation causes tetra-amelia in a large consanguineous family."
Niemann S., Zhao C., Pascu F., Stahl U., Aulepp U., Niswander L., Weber J.L., Mueller U.
Am. J. Hum. Genet. 74:558-563(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN ARTTRA.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY009397 mRNA. Translation: AAG38657.1.
AB067628 mRNA. Translation: BAB70502.1.
BC112116 mRNA. Translation: AAI12117.1.
BC112118 mRNA. Translation: AAI12119.1.
BC114219 mRNA. Translation: AAI14220.1.
PIRA47536.
RefSeqNP_110380.1. NM_030753.4.
UniGeneHs.445884.
Hs.745220.

3D structure databases

ProteinModelPortalP56703.
SMRP56703. Positions 60-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113310. 1 interaction.
IntActP56703. 1 interaction.
STRING9606.ENSP00000225512.

Chemistry

BindingDBP56703.
ChEMBLCHEMBL6079.

PTM databases

PhosphoSiteP56703.

Polymorphism databases

DMDM14424477.

Proteomic databases

PaxDbP56703.
PRIDEP56703.

Protocols and materials databases

DNASU7473.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000225512; ENSP00000225512; ENSG00000108379.
GeneID7473.
KEGGhsa:7473.
UCSCuc002ikv.3. human.

Organism-specific databases

CTD7473.
GeneCardsGC17M044839.
HGNCHGNC:12782. WNT3.
MIM165330. gene.
273395. phenotype.
neXtProtNX_P56703.
Orphanet3301. Tetraamelia - multiple malformations.
PharmGKBPA37383.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG284879.
HOGENOMHOG000039529.
HOVERGENHBG001595.
InParanoidP56703.
KOK00312.
OMAMCGCDSH.
OrthoDBEOG7C8GJ8.
PhylomeDBP56703.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP56703.
CleanExHS_WNT3.
GenevestigatorP56703.

Family and domain databases

InterProIPR005817. Wnt.
IPR009141. Wnt3.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF18. PTHR12027:SF18. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01843. WNT3PROTEIN.
PR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiWNT3.
GenomeRNAi7473.
NextBio29272.
PROP56703.
SOURCESearch...

Entry information

Entry nameWNT3_HUMAN
AccessionPrimary (citable) accession number: P56703
Secondary accession number(s): Q2M237, Q9H1J9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2001
Last modified: April 16, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM