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Protein

Proto-oncogene Wnt-3

Gene

WNT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube (By similarity).By similarity

GO - Molecular functioni

  1. frizzled binding Source: UniProtKB
  2. receptor agonist activity Source: BHF-UCL

GO - Biological processi

  1. anterior/posterior axis specification Source: Ensembl
  2. axon guidance Source: Ensembl
  3. canonical Wnt signaling pathway Source: UniProtKB
  4. canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation Source: BHF-UCL
  5. canonical Wnt signaling pathway involved in osteoblast differentiation Source: BHF-UCL
  6. cell fate commitment Source: GO_Central
  7. cell morphogenesis Source: BHF-UCL
  8. cellular response to retinoic acid Source: UniProtKB
  9. dorsal/ventral axis specification Source: Ensembl
  10. embryonic forelimb morphogenesis Source: Ensembl
  11. embryonic hindlimb morphogenesis Source: Ensembl
  12. gamete generation Source: Ensembl
  13. head morphogenesis Source: Ensembl
  14. limb bud formation Source: BHF-UCL
  15. mammary gland epithelium development Source: UniProtKB
  16. mesoderm formation Source: Ensembl
  17. negative regulation of axon extension involved in axon guidance Source: Ensembl
  18. neuron differentiation Source: UniProtKB
  19. positive regulation of collateral sprouting in absence of injury Source: Ensembl
  20. positive regulation of gene expression Source: Ensembl
  21. Spemann organizer formation at the anterior end of the primitive streak Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_163710. WNT ligand biogenesis and trafficking.
REACT_18372. Class B/2 (Secretin family receptors).
REACT_200777. TCF dependent signaling in response to WNT.

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene Wnt-3
Alternative name(s):
Proto-oncogene Int-4 homolog
Gene namesi
Name:WNT3
Synonyms:INT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17, UP000005640: Unplaced

Organism-specific databases

HGNCiHGNC:12782. WNT3.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: Reactome
  2. extracellular region Source: Reactome
  3. extracellular space Source: BHF-UCL
  4. Golgi lumen Source: Reactome
  5. plasma membrane Source: Reactome
  6. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Tetraamelia, autosomal recessive1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.

See also OMIM:273395

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

MIMi273395. phenotype.
Orphaneti3301. Tetraamelia - multiple malformations.
PharmGKBiPA37383.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 355334Proto-oncogene Wnt-3PRO_0000041416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi80 ↔ 91By similarity
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi131 ↔ 139By similarity
Disulfide bondi141 ↔ 158By similarity
Disulfide bondi206 ↔ 220By similarity
Disulfide bondi208 ↔ 215By similarity
Lipidationi212 – 2121O-palmitoyl serine; by PORCNBy similarity
Disulfide bondi300 ↔ 315By similarity
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi330 ↔ 345By similarity
Disulfide bondi332 ↔ 342By similarity
Disulfide bondi337 ↔ 338By similarity

Post-translational modificationi

Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP56703.
PRIDEiP56703.

PTM databases

PhosphoSiteiP56703.

Expressioni

Gene expression databases

BgeeiP56703.
CleanExiHS_WNT3.
GenevestigatoriP56703.

Interactioni

Subunit structurei

Interacts with PORCN. Interacts with WLS.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FZD7O750843EBI-3644922,EBI-746917

Protein-protein interaction databases

BioGridi113310. 2 interactions.
IntActiP56703. 1 interaction.
STRINGi9606.ENSP00000225512.

Structurei

3D structure databases

ProteinModelPortaliP56703.
SMRiP56703. Positions 60-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP56703.
KOiK00312.
OMAiMCGCDSH.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP56703.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009141. Wnt3.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01843. WNT3PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56703-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPHLLGLLL GLLLGGTRVL AGYPIWWSLA LGQQYTSLGS QPLLCGSIPG
60 70 80 90 100
LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI
110 120 130 140 150
FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG
160 170 180 190 200
EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD
210 220 230 240 250
HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR
260 270 280 290 300
ESRGWVETLR AKYSLFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC
310 320 330 340 350
NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCIFHWCCYV SCQECIRIYD

VHTCK
Length:355
Mass (Da):39,645
Last modified:June 20, 2001 - v2
Checksum:i85D15F2C7884A64F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009397 mRNA. Translation: AAG38657.1.
AB067628 mRNA. Translation: BAB70502.1.
BC112116 mRNA. Translation: AAI12117.1.
BC112118 mRNA. Translation: AAI12119.1.
BC114219 mRNA. Translation: AAI14220.1.
CCDSiCCDS11505.1.
PIRiA47536.
RefSeqiNP_110380.1. NM_030753.4.
UniGeneiHs.445884.
Hs.745220.

Genome annotation databases

EnsembliENST00000225512; ENSP00000225512; ENSG00000108379.
ENST00000611547; ENSP00000478327; ENSG00000277626.
ENST00000616347; ENSP00000480990; ENSG00000277641.
GeneIDi7473.
KEGGihsa:7473.
UCSCiuc002ikv.3. human.

Polymorphism databases

DMDMi14424477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009397 mRNA. Translation: AAG38657.1.
AB067628 mRNA. Translation: BAB70502.1.
BC112116 mRNA. Translation: AAI12117.1.
BC112118 mRNA. Translation: AAI12119.1.
BC114219 mRNA. Translation: AAI14220.1.
CCDSiCCDS11505.1.
PIRiA47536.
RefSeqiNP_110380.1. NM_030753.4.
UniGeneiHs.445884.
Hs.745220.

3D structure databases

ProteinModelPortaliP56703.
SMRiP56703. Positions 60-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113310. 2 interactions.
IntActiP56703. 1 interaction.
STRINGi9606.ENSP00000225512.

Chemistry

BindingDBiP56703.
ChEMBLiCHEMBL6079.

PTM databases

PhosphoSiteiP56703.

Polymorphism databases

DMDMi14424477.

Proteomic databases

PaxDbiP56703.
PRIDEiP56703.

Protocols and materials databases

DNASUi7473.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225512; ENSP00000225512; ENSG00000108379.
ENST00000611547; ENSP00000478327; ENSG00000277626.
ENST00000616347; ENSP00000480990; ENSG00000277641.
GeneIDi7473.
KEGGihsa:7473.
UCSCiuc002ikv.3. human.

Organism-specific databases

CTDi7473.
GeneCardsiGC17M044839.
GeneReviewsiWNT3.
HGNCiHGNC:12782. WNT3.
MIMi165330. gene.
273395. phenotype.
neXtProtiNX_P56703.
Orphaneti3301. Tetraamelia - multiple malformations.
PharmGKBiPA37383.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP56703.
KOiK00312.
OMAiMCGCDSH.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP56703.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_163710. WNT ligand biogenesis and trafficking.
REACT_18372. Class B/2 (Secretin family receptors).
REACT_200777. TCF dependent signaling in response to WNT.

Miscellaneous databases

GeneWikiiWNT3.
GenomeRNAii7473.
NextBioi29272.
PROiP56703.
SOURCEiSearch...

Gene expression databases

BgeeiP56703.
CleanExiHS_WNT3.
GenevestigatoriP56703.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009141. Wnt3.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01843. WNT3PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of six novel human WNT genes."
    Testa T.T., Mossakowska D.E., Carter P.S., Hu E., Zhu Y., Kelsell D.P., Murdock P.R., Herrity N.C., Lewis C.J., Cross D.A., Culbert A.A., Reith A.D., Barnes M.R.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and characterization of human WNT3."
    Katoh M.
    Int. J. Oncol. 19:977-982(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene."
    Roelink H., Wang J., Black D.M., Solomon E., Nusse R.
    Genomics 17:790-792(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-333.
  5. "Homozygous WNT3 mutation causes tetra-amelia in a large consanguineous family."
    Niemann S., Zhao C., Pascu F., Stahl U., Aulepp U., Niswander L., Weber J.L., Mueller U.
    Am. J. Hum. Genet. 74:558-563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN ARTTRA.

Entry informationi

Entry nameiWNT3_HUMAN
AccessioniPrimary (citable) accession number: P56703
Secondary accession number(s): Q2M237, Q9H1J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2001
Last modified: February 4, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.