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P56689

- DPOL_THEGO

UniProt

P56689 - DPOL_THEGO

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Protein
DNA polymerase
Gene
pol, polA
Organism
Thermococcus gorgonarius
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. exonuclease activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Alternative name(s):
    TO POL
    Gene namesi
    Name:pol
    Synonyms:polA
    OrganismiThermococcus gorgonarius
    Taxonomic identifieri71997 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 773773DNA polymerase
    PRO_0000046488Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi428 ↔ 442
    Disulfide bondi506 ↔ 509

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Beta strandi13 – 2210
    Beta strandi25 – 328
    Beta strandi37 – 448
    Helixi45 – 473
    Helixi48 – 514
    Beta strandi55 – 584
    Beta strandi61 – 644
    Beta strandi67 – 759
    Beta strandi78 – 869
    Helixi92 – 10211
    Beta strandi106 – 1116
    Helixi116 – 1238
    Beta strandi137 – 1448
    Beta strandi148 – 1514
    Beta strandi157 – 1648
    Beta strandi167 – 1748
    Beta strandi181 – 1833
    Helixi187 – 20115
    Beta strandi204 – 2107
    Helixi211 – 2133
    Helixi215 – 22511
    Beta strandi233 – 2364
    Beta strandi240 – 2434
    Beta strandi245 – 2517
    Beta strandi255 – 2595
    Helixi260 – 2678
    Helixi275 – 2839
    Helixi292 – 30110
    Helixi305 – 33733
    Helixi341 – 3455
    Helixi349 – 36315
    Helixi374 – 3796
    Beta strandi395 – 40713
    Helixi408 – 4158
    Turni420 – 4223
    Beta strandi429 – 4335
    Turni435 – 4373
    Beta strandi440 – 4423
    Helixi448 – 46922
    Helixi473 – 49018
    Helixi493 – 4986
    Helixi507 – 53024
    Beta strandi535 – 54713
    Helixi553 – 56816
    Beta strandi577 – 59014
    Beta strandi593 – 5975
    Beta strandi603 – 6075
    Beta strandi613 – 6153
    Helixi617 – 63115
    Helixi636 – 65116
    Helixi657 – 6593
    Beta strandi662 – 6654
    Helixi670 – 6723
    Beta strandi675 – 6784
    Helixi679 – 68911
    Beta strandi697 – 7048
    Beta strandi707 – 7093
    Helixi710 – 7134
    Beta strandi714 – 7163
    Helixi717 – 7193
    Turni722 – 7243
    Helixi729 – 7357
    Helixi738 – 7403
    Helixi742 – 7465
    Turni747 – 7493
    Helixi752 – 7543
    Beta strandi758 – 7603
    Helixi766 – 7694

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TGOX-ray2.50A1-773[»]
    2VWJX-ray2.78A1-773[»]
    2VWKX-ray2.60A1-773[»]
    2XHBX-ray2.72A1-757[»]
    ProteinModelPortaliP56689.
    SMRiP56689. Positions 1-773.

    Miscellaneous databases

    EvolutionaryTraceiP56689.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    [Graphical view]
    PRINTSiPR00106. DNAPOLB.
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56689-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILDTDYITE DGKPVIRIFK KENGEFKIDY DRNFEPYIYA LLKDDSAIED    50
    VKKITAERHG TTVRVVRAEK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI 100
    KEHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKMLAF DIETLYHEGE 150
    EFAEGPILMI SYADEEGARV ITWKNIDLPY VDVVSTEKEM IKRFLKVVKE 200
    KDPDVLITYN GDNFDFAYLK KRSEKLGVKF ILGREGSEPK IQRMGDRFAV 250
    EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AIFGQPKEKV YAEEIAQAWE 300
    TGEGLERVAR YSMEDAKVTY ELGKEFFPME AQLSRLVGQS LWDVSRSSTG 350
    NLVEWFLLRK AYERNELAPN KPDERELARR RESYAGGYVK EPERGLWENI 400
    VYLDFRSLYP SIIITHNVSP DTLNREGCEE YDVAPQVGHK FCKDFPGFIP 450
    SLLGDLLEER QKVKKKMKAT IDPIEKKLLD YRQRAIKILA NSFYGYYGYA 500
    KARWYCKECA ESVTAWGRQY IETTIREIEE KFGFKVLYAD TDGFFATIPG 550
    ADAETVKKKA KEFLDYINAK LPGLLELEYE GFYKRGFFVT KKKYAVIDEE 600
    DKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL 650
    SKYEVPPEKL VIYEQITRDL KDYKATGPHV AVAKRLAARG IKIRPGTVIS 700
    YIVLKGSGRI GDRAIPFDEF DPAKHKYDAE YYIENQVLPA VERILRAFGY 750
    RKEDLRYQKT RQVGLGAWLK PKT 773
    Length:773
    Mass (Da):89,812
    Last modified:July 15, 1999 - v1
    Checksum:iF67AF04E875FBE44
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TGO X-ray 2.50 A 1-773 [» ]
    2VWJ X-ray 2.78 A 1-773 [» ]
    2VWK X-ray 2.60 A 1-773 [» ]
    2XHB X-ray 2.72 A 1-757 [» ]
    ProteinModelPortali P56689.
    SMRi P56689. Positions 1-773.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P56689.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    [Graphical view ]
    PRINTSi PR00106. DNAPOLB.
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structure of a thermostable type B DNA polymerase from Thermococcus gorgonarius."
      Hopfner K.-P., Eichinger A., Engh R.A., Laue F., Ankenbauer W., Huber R., Angerer B.
      Proc. Natl. Acad. Sci. U.S.A. 96:3600-3605(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiDPOL_THEGO
    AccessioniPrimary (citable) accession number: P56689
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 15, 1999
    Last modified: September 3, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Multifunctional enzyme

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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